Campylobacter jejuni atcc 33291

Two C. jejuni strains (2868 and 2871) isolated independently from poultry obtained from retail outlets in Malaysia [23 ] were used in this study. Whole genomes of the strains have been sequenced [24 (link)]. Campylobacter jejuni ATCC 33291 and Pseudomonas aeruginosa ATCC 27853 obtained from the American Type Culture Collection (Manassas, USA) were also used in this study. All the C. jejuni strains were maintained at -80°C in nutrient broth no. 2 (NB2, Oxoid, UK) and 15% glycerol and were resuscitated on Campylobacter blood-free selective agar base (Oxoid, UK) with incubation at 37°C for 48 h under microaerobic conditions (5% O₂, 10% CO₂ and 85% N₂) generated using Campygen (Oxoid, UK) in an anaerobic jar (Oxoid, UK). P. aeruginosa was maintained at -80°C in Luria-Bertani broth (LB, Oxoid, UK) and 15% glycerol and was resuscitated on LB agar (Oxoid, UK) with incubation at 37°C for 24 h under aerobic conditions.

Seven C. jejuni strains (2862, 2863, 2865, 2866, 2868, 2869 and 2871) isolated from poultry obtained from retail outlets in Malaysia as reported by Wieczorek et al. [17 (link)] were used in this study. Campylobacter jejuni ATCC 33291 obtained from the American Type Culture Collection was also used in this study. Whole genomes of three of the strains, 2865, 2868 and 2871, were sequenced as described in Teh et al. [18 (link)]. All the strains were maintained at −80 °C in Nutrient Broth No. 2 (NB2, Oxoid, UK) and 15% glycerol and were resuscitated on Campylobacter blood-free selective agar base (Oxoid, UK) (as sessile cultures) with incubation at 37 °C for 48 h under microaerobic conditions generated using Campygen (Oxoid, UK).