Alexa fluor 594 goat anti mouse antibody

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

The Alexa Fluor 594 goat anti-mouse antibody is a fluorescently labeled secondary antibody used for detection and visualization in immunoassays and other applications. It is specific for mouse primary antibodies and can be detected using a fluorescence microscope or other fluorescence detection systems.

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Lab products found in correlation

Alexa fluor 488 goat anti rabbit antibody, by Thermo Fisher Scientific (9 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (5 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (5 mentions) Hoechst 33342, by Thermo Fisher Scientific (3 mentions) Triton x 100, by Merck Group (3 mentions) Protein g agarose, by Thermo Fisher Scientific (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Lithium chloride (licl), by Merck Group (2 mentions) Mg132, by Merck Group (2 mentions) Ab17721 and ab90966, by Abcam (2 mentions) Glutathione s transferase beads, by Merck Group (2 mentions) Ab4812, by Abcam (2 mentions) α tubulin sc 5286, by Santa Cruz Biotechnology (2 mentions) Ab48031, by Abcam (2 mentions) Myc tag 2276, by Cell Signaling Technology (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 donkey anti goat antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 goat anti mouse antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 donkey anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Agilent Technologies (2 mentions)

Close spelling variants of this product

Alexa Fluor 594 (2045 citations) Alexa 594 (538 citations) Alexa Fluor 594 goat anti-mouse (177 citations) Alexa Fluor 594 anti-mouse (84 citations) Goat anti-mouse Alexa 594 (49 citations) Alexa Fluor 594-conjugated goat anti-mouse antibody (22 citations) Alexa Fluors (21 citations) Alexa Fluor 594-conjugated goat anti-mouse secondary antibody (20 citations) Alexa Fluor 594 goat anti-mouse secondary antibody (16 citations) Alexa Fluor 594 goat anti-mouse IgG secondary antibody (13 citations)

37 protocols using alexa fluor 594 goat anti mouse antibody

Antibody-Based Protein Detection Assay

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Antibodies against human KDM1A (ab17721 and ab90966), CK1δ (ab48031), GATA6 (ab22600), and USP22 (ab4812) were from Abcam. Antibodies against GST (sc-138), Tuj-1(sc-58888), CK1α (sc-6477), CK1ε (sc-6471), BMP2 (sc-6895), CDKN1A (sc-397), and α-Tubulin (sc-5286) were from Santa Cruz Biotechnology. Antibodies against human GSK3β (9315), GSK3α (9338), CD133 (3663), phospho-GSK3β-S9 (9336), Oct4 (2750), H3K4me2/3 (9725 and 9751) and Myc-tag (2276) were from Cell Signaling Technology. Antibodies against phospho-serine/threonine (612548) and nestin (611658) were from BD Transduction Laboratories. Antibodies against hemagglutinin (HA)-Tag and Flag-tag were from Sigma. Hoechst 33342, Alexa Fluor 488 goat anti-rabbit antibody, and Alexa Fluor 594 goat anti-mouse antibody were from Molecular Probes. The specific antibody against phospho-KDM1A S683 was produced by Signalway Antibody. Supplementary Table 1 contains detailed information about the used antibodies.
Temozolomide (TMZ), tideglusib, lithium chloride, glutathione S-transferase (GST) beads, cycloheximide (CHX), and MG132 were from Sigma. Puromycin, hygromycin, and D4476 (4-[4-(2,3-dihydro-benzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl] benzamide) were from EMD Biosciences. Protein G agarose was from Life Technologies. The recombinant active GSK3β (G09-10G) and CK1α (C64-10G) proteins were from SignalChem Lifesciences Corp.

Zhou A., Lin K., Zhang S., Chen Y., Zhang N., Xue J., Wang Z., Aldape K.D., Xie K., Woodgett J.R, & Huang S. (2016). Nuclear GSK3β promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitination by USP22. Nature cell biology, 18(9), 954-966.

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Antibody-Based Protein Detection Assay

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Histological Analysis of Murine Hearts

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Hearts from mice at 1 week and 8 weeks after TAC were fixed with formalin, embedded with paraffin and cut into 4 μm slices for histological analysis. The size of heart was assessed by histological analysis with hematoxylin / eosin (HE) staining and Masson staining.
Paraffin-embedded tissue sections were stained with mouse monoclonal α-sarcomeric actin (α-actin) antibody (1:75, Abcam) and Alexa Fluor 594 goat anti-mouse antibody (1:200, Molecular Probes) as secondary antibody. DAPI (4’-6-diamidino- 2-phenylindole, Sigma) were used as nuclei marker. Sections were mounted and analyzed with a fluoview 1000 confocal microscope (Olympus, Japan).

Li Q., Xie J., Wang B., Li R., Bai J., Ding L., Gu R., Wang L, & Xu B. (2016). Overexpression of microRNA-99a Attenuates Cardiac Hypertrophy. PLoS ONE, 11(2), e0148480.

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Immunofluorescence Staining for Neurofilament 200

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The cells were seeded on coverglass supports in complete medium and treated with RA for the indicated times. The cells were fixed with 4% (w/v) paraformaldehyde and permeabilised in PBS containing 0.1% Triton-X 100. NF200 was detected using an anti-NF200 monoclonal antibody (N52; Sigma-Aldrich). Alexa Fluor 594 goat anti-mouse antibody (Molecular Probes, Paisley, UK) was used as a secondary antibody. The antibodies were diluted in PBS. Cell nuclei were stained with 1 mg/ml DAPI or Hoechst 33342 in PBS for 5 min. Finally, the cells were washed in PBS and briefly rinsed in ddH₂O, and the coverglass slips were mounted in ProLong Gold anti-Fade Reagent (Molecular Probes). Images were acquired with an Olympus BX51 fluorescence microscope and analysed with I.A.S. software (Delta Sistemi, Legnano (VR), Italy). The brightness and contrast of the acquired images were adjusted, and the figures were generated using Adobe Photoshop 7.0.

Guglielmi L., Cinnella C., Nardella M., Maresca G., Valentini A., Mercanti D., Felsani A, & D'Agnano I. (2014). MYCN gene expression is required for the onset of the differentiation programme in neuroblastoma cells. Cell Death & Disease, 5(2), e1081-.

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Antibody Panel for Protein Analysis

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Mouse monoclonal antibodies for PFKM (sc-67028, 1:1,000 for immunoblotting), β-catenin (E-5, sc-7963, 1:200 for immunoblotting and 1:50 for immunofluorescence), and c-Myc (9E10, sc-40, 1:200 for immunoblotting) and polyclonal antibody for cyclin D1 (H-295, sc-753, 1:200 for immunoblotting) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit polyclonal antibodies recognizing PFKP (12746, 1:1,000 for immunoblotting), PFKL (8175, 1:1,000 for immunoblotting), and β-catenin pS552 (9566, 1:1,000 for immunoblotting) were purchased from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibody for tubulin (clone B-5-1-2, T6074, 1:5,000 for immunoblotting) was purchased from Sigma (St. Louis, MO). Mouse monoclonal antibody for PCNA (610665, 1:1,000 for immunoblotting was purchased from BD Biosciences (San Jose, CA). Human recombinant EGF (01-407) was obtained from EMD Millipore (Billerica, MA). Hygromycin (400053), puromycin (540222), and G418 (345810) were purchased from EMD Biosciences (San Diego, CA). HyFect transfection reagents (E2650) were obtained from Denville Scientific (Metuchen, NJ). DAPI and Alexa Fluor 594 goat anti-mouse antibody were purchased from Molecular Probes (Eugene, OR).

Lee J.H., Shao F., Ling J., Lu S., Liu R., Du L., Chung J.W., Koh S.S., Leem S.H., Shao J., Xing D., An Z, & Lu Z. (2020). Phosphofructokinase 1 Platelet Isoform Promotes β-Catenin Transactivation for Tumor Development. Frontiers in Oncology, 10, 211.

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Transfection and Signaling Pathway Analysis

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RPMI-1640 medium, fetal bovine serum (FBS), phosphate-buffered saline (PBS), and antibiotics (penicillin and streptomycin) were purchased from Welgene Inc. (Seoul, Korea). Lipofectamine™ 2000 was purchased from Invitrogen (Carlsbad, CA, USA). JetPEI was purchased from Polyplus-transfection (Illkirch-Graffenstaden, France). SPC was purchased from Matreya (Pleasant Gap, PA, USA). PD98059, SP600125, and SB203580 were purchased from Calbiochem (La Jolla, CA, USA). Anti-YDJC, anti-K8, and the phosphospecific antibody to detect K8S431 were obtained from Abcam (Cambridge, MA, USA). Anti-ERK and the phosphospecific antibody to detect ERK1/2 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-CDC16 and anti-β-actin antibody were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Alexa Fluor 488 goat anti-rabbit antibody and Alexa Fluor 594 goat anti-mouse antibody were obtained from Molecular Probes, Inc. (Eugene, OR, USA). All the chemicals were freshly prepared at the time of each experiment.

Kim E.J., Park M.K., Byun H.J., Kang G.J., Yu L., Kim H.J., Shim J.G., Lee H, & Lee C.H. (2018). YdjC chitooligosaccharide deacetylase homolog induces keratin reorganization in lung cancer cells: involvement of interaction between YDJC and CDC16. Oncotarget, 9(33), 22915-22928.

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Podoplanin Immunofluorescence Staining in H226 and 211H Cells

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H226 and 211H cells were seeded on coverslips, incubated overnight, and fixed with 4% paraformaldehyde the next day. Immunofluorescence staining was conducted using anti‐podoplanin antibody LpMab‐17 as a primary antibody with an Alexa Fluor 594 goat antimouse antibody (Molecular Probes, Eugene, OR, USA) as a secondary antibody. Coverslips were placed on glass slides using mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA). Fluorescence images were captured with an exposure time of 50 ms for podoplanin and 25 ms for DAPI using a fluorescence microscope BX53 (Olympus, Tokyo, Japan) and cellSens Standard software (ver. 1.7.1; Olympus).

Sudo H., Tsuji A.B., Sugyo A., Saga T., Kaneko M.K., Kato Y, & Higashi T. (2019). Therapeutic efficacy evaluation of radioimmunotherapy with 90Y‐labeled anti‐podoplanin antibody NZ‐12 for mesothelioma. Cancer Science, 110(5), 1653-1664.

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Phosphorylation status of Keratin 8

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TPA was purchased from Cell Signaling Technology (Beverly, MA, USA). The phosphospecific antibody for detection of K8 Ser431, K8 Ser23, and K8 Ser73 was purchased from Abcam (Cambridge, UK). The anti-Tgase-2 and anti-β-actin antibodies were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA), as were peroxidase-labeled secondary antibodies. Alexa Fluor 488 goat anti-rabbit antibody and Alexa Fluor 594 goat anti-mouse antibody were obtained from Molecular Probes, Inc. (Eugene, OR, USA). All of these chemicals were freshly prepared for each experiment.

Lee E.J., Park M.K., Kim H.J., Kang J.H., Kim Y.R., Kang G.J., Byun H.J, & Lee C.H. (2014). 12-O-Tetradecanoylphorbol-13-Acetate Induces Keratin 8 Phosphorylation and Reorganization via Expression of Transglutaminase-2. Biomolecules & Therapeutics, 22(2), 122-128.

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Analyzing Apoptosis and Oxidative Stress

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Rabbit monoclonal anti-p-MAPK antibody, rabbit monoclonal anti-LC3 antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). Alexa Fluor 488 goat anti-rabbit antibody and Alexa Fluor 594 goat anti-mouse antibody were purchased from Invitrogen (Carlsbad,CA). Dihydroethidium was from the Beyotime Institute of Biotechnology (Nantong, China). Annexin V-FITC/EGFP Apoptosis Detection Kits were from Vazyme Biotech Co.Ltd. (Nanjing, China). HT-2 toxin was from J&K Chemical Ltd. (Shanghai, China). Other chemicals were from Sigma Chemical Company (St. Louis, MO).

Zhang Y., Han J., Zhu C.C., Tang F., Cui X.S., Kim N.H, & Sun S.C. (2016). Exposure to HT-2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes. Scientific Reports, 6, 33904.

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Immunofluorescence Analysis of Androgen Receptor

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The AR antibody (Catalog: SC-816-G) was from Santa Cruz Biotechnology (Paso Robles, US). Testosterone and DHT were obtained from Sigma (Poole, UK). The SMA antibody (Catalog: A2547) was from sigma. DAPI, Alexa Fluor@488 donkey-anti goat antibody (Catalog: A-11055) and Alexa Fluor@594 goat-anti mouse antibody (Catalog: A-11005) were from Invitrogen (Paisley, UK).

Zhu D., Hadoke P.W., Wu J., Vesey A.T., Lerman D.A., Dweck M.R., Newby D.E., Smith L.B, & MacRae V.E. (2016). Ablation of the androgen receptor from vascular smooth muscle cells demonstrates a role for testosterone in vascular calcification. Scientific Reports, 6, 24807.

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