Rabbit anti pax6

Manufactured by Proteintech

Rabbit anti-Pax6 is a primary antibody that specifically recognizes the Pax6 protein. Pax6 is a transcription factor involved in the regulation of gene expression during development.

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Lab products found in correlation

4 6 diamidino 2 phenylindole dapi, by Cayman Chemical (1 mentions) Mouse anti pax6, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti mouse igg, by Proteintech (1 mentions) Rabbit anti ctip2, by Abcam (1 mentions) Rabbit anti tbr1, by Proteintech (1 mentions) Alexa fluor 555 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Rat anti brdu, by Abcam (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rat igg, by Jackson ImmunoResearch (1 mentions) Rabbit anti ki67, by Abcam (1 mentions) Tunel fitc apoptosis detection kit, by Vazyme (1 mentions) Mouse anti gfap, by Proteintech (1 mentions) Rabbit anti nestin, by Merck Group (1 mentions) Sp8 confocal microscope, by Leica camera (1 mentions)

2 protocols using rabbit anti pax6

Immunostaining Markers in Neurodevelopment

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Primary antibodies used in this study include: rabbit anti-Brn2 (Santa Cruz, 1:50), rabbit anti-Ctip2 (Abcam, 1:1000), rabbit anti-Tbr1 (Proteintech Group, 1:500), rat anti-BrdU (Abcam, 1:1000), rabbit anti-Ki67 (Abcam, 1:500), mouse anti-Pax6 (Cell Signaling Technology, 1:200), rabbit anti-Pax6 (Proteintech Group, 1:1000). Secondary antibodies used include: Alexa Fluor 555 donkey anti-rabbit IgG (Invitrogen, 1:300), Alexa Fluor 488 donkey anti-rabbit IgG (Invitrogen, 1:300), Alexa Fluor 488 goat anti-mouse IgG (Proteintech Group, 1:300), Alexa Fluor 488 goat anti-rat IgG (Jackson Immuno Research, 1:300). Cell apoptosis was detected by TUNEL FITC Apoptosis Detection Kit (Vazyme, A111). Nuclei were counterstained with 4, 6-diamidino-2-phenylindole (DAPI; Cayman Chemical).

Mao R., Deng R., Wei Y., Han L., Meng Y., Xie W, & Jia Z. (2019). LIMK1 and LIMK2 regulate cortical development through affecting neural progenitor cell proliferation and migration. Molecular Brain, 12, 67.

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Immunofluorescence Assay for Stem Cells

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An IF assay was performed based on our previous method 16. Briefly, undifferentiated H9 cells and NSCs derived from H9 cells were maintained on Matrigel-coated coverslips. Cells were incubated with mouse OCT4 (1:100, Santa Cruz Biotechnology), rabbit anti-PAX6 (1:100, Proteintech), rabbit anti-NESTIN (1:200, Sigma), mouse anti-GFAP (1:500, Proteintech) and goat anti-SOX1 (1:100, Bio-techne). Alexa Fluor 594 anti-rabbit (1:500), Alexa Fluor 488 anti-mouse (1:500) and anti-goat (1:500) antibodies were used as secondary antibodies. Visualisation of IF results was performed using a Leica SP8 confocal microscope.

Liu C., Xie Y., Chen X., Liu L., Liu C, & Yin Z. (2024). BAF45D-binding to HOX genes was differentially targeted in H9-derived spinal cord neural stem cells. Scientific Reports, 14, 29.

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