Eicosapentaenoic acid

Phytomonic acid (C18 CFA), palmitoleic acid (C16∶1), palmitic acid (C16∶0), oleic acid (C18∶1n9), and stearic acid (C18∶0) were obtained from Cayman Chemical (MI). Lauric acid (C12∶0), myristic acid (C14∶0), vaccenic acid (C18∶1n7), elaidic acid (trans-C18∶1n9), linoleic acid (C18∶2n6), arachidonic acid (C20∶4n6), and eicosapentaenoic acid (C20∶5n3) were from Nu-Chek Prep, Inc. (MN). All solvents were of GC-MS grade and all other reagents were of the highest grade available.

TWM was obtained from Qianjin Xieli Pharmaceutical Co., Ltd (Hunan, China). TP (>98% pure) was provided by Guilin Sanjin Biologics Co., Ltd (Guanxi, China). Docosahexaenoic acid (C22:6n3), docosanoic acid (C22:0), eicosapentaenoic acid (C20:5n3), eicosatrienoic acid (C20:3n6), arachidonic acid (C20:4n6), arachidic acid (C20:0), nonadecadienoic acid (C19:2n6), γ-linolenic acid (C18:3n6), α-linolenic acid (C18:3n3), linoleic acid (C18:2n6), oleic acid (C18:1n9), cis-vaccenic acid (C18:1n7), stearic acid (C18:0), palmitic acid (C16:0), palmitoleic acid (C16:1n7), myristic acid (C14:0) and lauric acid (C12:0) were purchased from Nu-Chek Prep (Elysian, MN, USA). Their purities were above 98%. Trimethylsilane diazomethane (TMSCHN₂) in n-hexane (2 mol/L) was purchased from Energy Chemical (Shanghai, China). The other chemical reagents were purchased from Xilong Scientific Co., Ltd (Guangdong, China).