Advia 2400 system

The HIL index was measured automatically by the ADVIA-2400 system (Siemens Healthcare Diagnostics). The HIL index were measured automatically by the ADVIA-2400 system (Siemens Healthcare Diagnostics). The serum HIL index feature of the ADVIA-2400 chemistry system can detect and produce a qualitative estimate absorbance value of three sets of wavelengths: hemolysis (λ₁ = 571 nm, λ₂ = 596 nm), lipemia (λ₁ = 658 nm, λ₂ = 694 nm), and icterus (λ₁ = 478 nm, λ₂ = 505 nm) [11 (link)]. The concentration ranges of HIL index value were set as follows: hemoglobin, < 45 mg/dL (-), 45–140 mg/dL (+), 140–235 mg/dL (++), 235–445 mg/dL (+++) and > 445 mg/dL (++++); lipemia, < 120 mg/dL (-), 120~245 mg/dL (+), 245~495 mg/dL (++), 495–995 mg/dL (+++) and > mg/dL 995 (++++); icterus, < 1.60 mg/dL (-), 1.60–6.50 mg/dL (+), 6.50–15.0 mg/dL (++), 15.0–28.0 mg/dL (+++) and > 28.0 mg/dL (++++). Clinical and Laboratory Standards Institute (CLSI) document C56A guides the use of serum indices to measure HIL interference and recommends the selection of assay-specific HIL cut-offs, above which HIL interferences will affect results. The HIL index in the study is defined as the lowest concentrations of HIL that interfere with chemical analyses, yielding a bias >10%.