Cyclic di amp elisa kit

Manufactured by Cayman Chemical

The Cyclic-di-AMP ELISA Kit is a laboratory tool used to detect and quantify the presence of cyclic-di-AMP, a signaling molecule, in samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the target analyte.

Automatically generated - may contain errors

Lab products found in correlation

M per, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Cyclic AMP ELISA Kit ELISAs

2 protocols using cyclic di amp elisa kit

Quantitative Analysis of c-di-AMP Levels in S. pneumoniae

Check if the same lab product or an alternative is used in the 5 most similar protocols

S. pneumoniae strains used in this study were derived from D39 Δcps (58 (link)) unless otherwise stated. S. pneumoniae cells were grown in Todd Hewitt broth containing 0.5% yeast extract at 37 °C with 5% CO₂. Strains, plasmids, and oligonucleotides used in this study are given in SI Appendix, Table S1. The c-di-AMP levels were quantified using a “Cyclic-di-AMP ELISA Kit” from Cayman Chemical (item No. 501960) in accordance with the manufacturer’s instructions. More detailed protocols are given in SI Appendix file.

Kobras C.M., Monteith W., Somerville S., Delaney J.M., Khan I., Brimble C., Corrigan R.M., Sheppard S.K, & Fenton A.K. (2023). Loss of Pde1 function acts as an evolutionary gateway to penicillin resistance in Streptococcus pneumoniae. Proceedings of the National Academy of Sciences of the United States of America, 120(41), e2308029120.

+ Open protocol

+ Expand

Quantifying Cyclic di-AMP in Infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa cells were infected with OncoVACV or STINGPOX (MOI = 0.1) and 48 hours post-infection cells and supernatants were harvested. Cells were lysed in mammalian protein extraction reagent (M-PER; Thermo Scientific) and boiled at 95°C for 5 minutes. Cyclic di-AMP levels were measured in the cell lysate and supernatant via the cyclic di-AMP ELISA kit (Cayman Chemicals), as per manufacturer’s protocol.

Whelan J.T., Singaravelu R., Wang F., Pelin A., Tamming L.A., Pugliese G., Martin N.T., Crupi M.J., Petryk J., Austin B., He X., Marius R., Duong J., Jones C., Fekete E.E., Alluqmani N., Chen A., Boulton S., Huh M.S., Tang M.Y., Taha Z., Scut E., Diallo J.S., Azad T., Lichty B.D., Ilkow C.S, & Bell J.C. (2023). CRISPR-mediated rapid arming of poxvirus vectors enables facile generation of the novel immunotherapeutic STINGPOX. Frontiers in Immunology, 13, 1050250.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!