Macro prep cm cation exchange resin

7-Hydroxy-4-(trifluoromethyl)coumarin (7-HFC), and 7-EFC were purchased from Life Technologies (Carlsbad, CA). β-NADPH, RNase A, DNase I, resorufin, and 7-BR were purchased from Sigma-Aldrich (St. Louis, MO). Ni²⁺-NTA affinity resin was purchased from Qiagen (Valencia, CA), and Macro-Prep CM cation exchange resin was obtained from Bio-Rad Laboratories, Inc. (Hercules, CA). The QuikChange XL site-directed mutagenesis kit and TOPP3 cells were obtained from Agilent Technologies (Santa Clara, CA). Recombinant NADPH:cytochrome P450 reductase (POR) [29 (link)] and cytochrome b₅ (b₅) from rat liver [30 (link), 31 (link)] were prepared as described previously. All other chemicals and supplies used were from standard sources. All protein model figures were created using MacPyMOL [32 ]. Channel/cavity analysis was performed using the Graphical User Interface for the Mole 2.0 program on Windows [33 (link)].

Amlodipine besylate, β-NADPH, ribonuclease A (RNase), deoxyribonuclease I (DNase), resorufin, and 7-BR were purchased from Sigma-Aldrich (St. Louis, MO). 7- Hydroxy-4-(trifluoromethyl) coumarin (7-HFC), and 7-EFC were purchased from Invitrogen (Carlsbad, CA). Nickel-nitrilotriacetic acid affinity resin was from Qiagen (Valencia, CA), and Macroprep CM cation exchange resin was obtained from Bio-Rad Laboratories (Hercules, CA). The QuikChange XL site-directed mutagenesis kit and TOPP3 and JM109 cells were obtained from Stratagene (La Jolla, CA). The molecular chaperone plasmid pGro7, which expresses GroES/EL, was obtained from TAKARA BIO (Shiba, Japan). Recombinant NADPH cytochrome P450 reductase (CPR) and cytochrome b₅ from rat liver were prepared as described previously [32 (link)]. All other chemicals and supplies used were from standard sources.