Adult male mice were anesthetized by an intraperitoneal injection of 50 mg/kg pentobarbital sodium. Before the operation, 1% tropicamide eye drops (Santen Pharmaceutical Co., Ltd., Osaka, Japan) were used to dilate the pupils, and 0.5% ropivacaine hydrochloride (Santen Pharmaceutical) was used for corneal anesthesia. Then, a 33-gauge needle connected to a saline solution was inserted into the anterior chamber in a single pass to stabilize the IOP between 110 and 120 mm Hg for 60 minutes. The animal model was evaluated by monitoring IOP and retinal vessel color. IOP was measured as previously described by Chen et al.17 (link) (Supplementary Data S1 ), and retinal ischemia was confirmed by microscopy (Supplementary Data S2 ). The RIR model was performed in only one eye for each mouse, and the contralateral eye was untreated. A sham procedure was performed in a separate group with cannulation of one eye without elevating the pressure (Supplementary Data S3 ). Carbomer eye drops (Bausch + Lomb, Bridgewater, NJ, USA) were used to moisten the eyes during ischemic injury. After 60 minutes of retinal ischemia, the needle was carefully withdrawn to normalize the IOP and restore retinal vascular perfusion, and ofloxacin ointment (Santen Pharmaceutical) was applied to the experimental eye to prevent bacterial infection.
Ofloxacin ointment
Manufactured by Santen
Sourced in Japan
Ofloxacin ointment is a topical antibiotic medication used for the treatment of bacterial eye infections. It is a broad-spectrum fluoroquinolone antibiotic that works by inhibiting bacterial DNA synthesis, leading to the death of the bacteria. The ointment formulation is designed for direct application to the affected eye area.
Automatically generated - may contain errors
Lab products found in correlation
Tropicamide eye drop, by Santen (1 mentions)
Betamethasone sodium phosphate, by Santen (1 mentions)
Levofloxacin hydrate ophthalmic solution, by Santen (1 mentions)
Xylazine, by Bayer (1 mentions)
Ketamine, by Daiichi Sankyo (1 mentions)
Stereomicroscope, by Olympus (1 mentions)
Butorphanol tartrate, by Meiji Seika Pharma (1 mentions)
Medetomidine hydrochloride, by Nippon Zenyaku Kogyo (1 mentions)
4 protocols using ofloxacin ointment
1
Retinal Ischemia-Reperfusion Injury Model
2
Intravitreal RSV Injection in Mice
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Mice were administered 1 µl of 3-µM or 30-µM RSV (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) dissolved in PBS by injection into the right eye with a 35-gauge needle in the vitreous body on the same day after the microbead injection. As control, 1 µl of PBS was injected into the left eye. To prevent infection, ofloxacin ointment (Santen Pharmaceutical Co., Osaka, Japan) was applied.
3
Glaucoma Filtration Surgery in Rabbits
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For the glaucoma filtration surgery model, rabbits were anesthetized with an intramuscular injection of ketamine (15 mg/kg) (Daiichi Sankyo Propharma) and xylazine (10 mg/kg) (Bayer Yakuhin). An eyelid speculum was put in place, and the eye fixed with a corneal traction suture (7–0 Silk, Johnson & Johnson). We then injected xylocaine (0.3–0.5 ml) under the Tenon tissue as an additional local anesthesia, and made a 6-mm fornix-based conjunctival flap with the Tenon’s capsule, 5 mm in length. Next, we made a 3 × 3 mm fornix-based scleral flap and placed 0.04% mitomycin C (Kyowa Kirin) into the conjunctival pocket for 3 min, which was then washed away with 50 ml of saline. Finally, we created a 2 × 1 mm scleral window at the limbus and performed a peripheral iridectomy, followed by a conjunctival suture with 10–0 nylon (MANI) and the injection of 0.5 ml dexamethasone into the subconjunctiva. After surgery, 0.3% ofloxacin ointment (Santen Pharmaceutical) was applied to the eye. Topical administrations of 0.1% betamethasone sodium phosphate (SHIONOGI) and 0.5% levofloxacin hydrate ophthalmic solution (Santen Pharmaceutical) were applied three times a day for three days postoperatively. All surgeries were performed by the same researcher (K.M).
4
Rat Lacrimal Gland Excision Model
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All animal experiments were performed in accordance with ARRIVE guidelines and the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the animal ethics committee of Osaka University (certificate number 02–024-000). Male Slc:SD rats (body weight 200–250 g, aged 7 weeks, SLC, Inc., Tokyo, Japan) were anaesthetised with an intraperitoneal injection of 0.15 mg kg−1 medetomidine hydrochloride (Nippon Zenyaku Kogyo, Fukui, Japan), 2.0 mg kg−1 midazolam (Maruishi Pharmaceutical, Osaka, Japan) and 2.5 mg kg−1 butorphanol tartrate (Meiji Seika Pharma, Tokyo, Japan). Under deep anaesthesia, the exorbital and intraorbital lacrimal glands were excised only on the left side, after which the incisions were sutured with an 8–0 nylon thread using a stereomicroscope (Olympus). After surgery, 0.3% ofloxacin ointment (Santen Pharmaceutical, Osaka, Japan) and 0.1% betamethasone sodium phosphate ointment (Shionogi Pharmaceutical) were administered twice daily.
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