Type 1 dextrin from corn

Type I dextrin from corn (M_w = 7500), colistin sulfate, α-amylase from human saliva, N-hydroxysulfosuccinimide (sulfo-NHS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), bicinchoninic acid (BCA) solution, dimethyl sulfoxide (DMSO), glucose and maltose oligomers (DP 2, 3, 6 and 7) were purchased from Sigma-Aldrich (Poole, UK). 1-Ethyl-3-(3-(dimethylamino)propyl carbodiimide hydrochloride) (EDC) was acquired from Pierce (Rockford, USA). Disodium hydrogen phosphate, potassium dihydrogen phosphate, potassium chloride, 4-dimethylaminopyridine (DMAP), and sodium chloride were from Fisher Scientific (Loughborough, UK). Unless otherwise stated, all chemicals were of analytical grade and used as received. All solvents were of general reagent grade (unless stated) and were from Fisher Scientific (Loughborough, UK). Colistimethate sodium (CMS, Colomycin®) was from Teva UK Limited (Eastbourne, UK).

Type I dextrin from corn (M_w = 7500 g mol⁻¹, degree of polymerisation = 50), colistin sulfate, N-hydroxysulfosuccinimide (sulfo-NHS) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Poole, UK). QIAzol reagent and miRNeasy kit were from Qiagen (Manchester, UK). BCA protein assay kit, N,N′-dicyclohexyl carbodiimide (DCC), 1-ethyl-3-(3-(dimethylamino)propyl carbodiimide hydrochloride) (EDC), Gibco™-branded keratinocyte serum-free medium (K-SFM) with l-glutamine, epidermal growth factor (EGF), bovine pituitary extract (BPE), Dulbecco's modified Eagle's medium (DMEM) with high glucose (4.5 g L⁻¹) and GlutaMAX™, fetal bovine serum (FBS), 0.05% w/v trypsin-0.53 mM EDTA, Oregon Green™ 488 (OG) cadaverine, OG carboxylic acid, wheat germ agglutinin-Alexa 594 (WGA-AF594), leupeptin, Hoechst 33342, BODIPY™ TR Ceramide complexed to bovine serum albumin (BSA), Mitotracker™ Red FM, high capacity cDNA kit and power SYBR green were obtained from ThermoFisher Scientific (Loughborough, UK). Narrow (polyethylene oxide, M_w = 24 063 g mol⁻¹, PDI = 1.02) and broad (dextran, M_w = 68 162 g mol⁻¹, PDI = 1.24) PolyCAL™ standards were purchased from Malvern Panalytical (Malvern, UK). Unless otherwise stated, all chemicals were of analytical grade and used as supplied. All solvents were of general reagent grade (unless stated) and were from Fisher Scientific (Loughborough, UK).