Recombinant human interleukin 4 rhil 4

Roswell Park Memorial Institute (RPMI) 1640 culture medium and fetal bovine serum (FBS) were purchased from HyClone (Logan, UT, USA). Recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin-4 (rhIL-4) were purchased from PeproTech (Rocky Hill, CT, USA). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich Co (St Louis, MO, USA). Fluorescein isothiocyanate (FITC)-conjugated anti-CD80, anti-CD83, and phycoerythrin (PE)-conjugated anti-CD86, anti-HLA-DR were purchased from BioLegend (San Diego, CA, USA). Anti-B7-H1 polyclonal antibody was purchased from Santa Cruz Biotech (Dallas, TX, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Tokyo, Japan). IL-10 enzyme-linked immunosorbent assay (ELISA) kit, IL-12 ELISA kit, and IFN-γ ELISA kit were purchased from R&D Systems (Minneapolis, MN, USA). Lactate dehydrogenase (LDH) cytotoxicity assay kit was purchased from Beyotime (Nanjing, People’s Republic of China).