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Mab3448

Manufactured by Merck Group
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Sourced in Germany

Mab3448 is a laboratory equipment product manufactured by Merck Group. It is designed for a specific function within the laboratory environment. The core function of Mab3448 is to facilitate a particular process or analysis, without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Ab8898, by Abcam (3 mentions) Ab48352, by Abcam (2 mentions) Ab2828, by Abcam (2 mentions) Ab10483, by Abcam (2 mentions) Ab12317, by Abcam (2 mentions) Ab62739, by Abcam (2 mentions) A9044, by Merck Group (1 mentions) A4914, by Merck Group (1 mentions) Ab11169, by Abcam (1 mentions) Ab21083, by Abcam (1 mentions) Ab9045, by Abcam (1 mentions) Ab80779, by Abcam (1 mentions) Ab41951, by Abcam (1 mentions) Ab7091, by Abcam (1 mentions) Ab1220, by Abcam (1 mentions) Ab2893, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) P0399, by Agilent Technologies (1 mentions)

4 protocols using mab3448

1

Episomal Plasmid Reporters for L1 Study

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The episomal L1-EGFP reporter plasmid and the L1 5′UTR-Luciferase reporter plasmid used in this study were both described previously25 (link),50 (link). Both constructs were kindly provided by John V. Moran. Plasmids encoding SIRT6, SIRT6 mutants, KAP1 and HPRT were cloned by amplifying the corresponding cDNA from HDF cells and then ligating the amplicon into a NotI/SalI digested pEGFP-N1 backbone.
The following antibodies were used in this study: 1:500 SIRT6 (ab48352, ab62739; Abcam), 1:500 KAP1 (ab10483, Abcam), 1:500 SETDB1 (ab12317, Abcam), 1:500 MeCP2 (ab2828, Abcam), 1:1000 H3K9me3 (ab8898, Abcam), 1:1000 HP1α (mab3448, Millipore).
Van Meter M., Kashyap M., Rezazadeh S., Geneva A.J., Morello T.D., Seluanov A, & Gorbunova V. (2014). SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and age. Nature communications, 5, 5011.
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2

Episomal Plasmid Reporters for L1 Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
The episomal L1-EGFP reporter plasmid and the L1 5′UTR-Luciferase reporter plasmid used in this study were both described previously25 (link),50 (link). Both constructs were kindly provided by John V. Moran. Plasmids encoding SIRT6, SIRT6 mutants, KAP1 and HPRT were cloned by amplifying the corresponding cDNA from HDF cells and then ligating the amplicon into a NotI/SalI digested pEGFP-N1 backbone.
The following antibodies were used in this study: 1:500 SIRT6 (ab48352, ab62739; Abcam), 1:500 KAP1 (ab10483, Abcam), 1:500 SETDB1 (ab12317, Abcam), 1:500 MeCP2 (ab2828, Abcam), 1:1000 H3K9me3 (ab8898, Abcam), 1:1000 HP1α (mab3448, Millipore).
Van Meter M., Kashyap M., Rezazadeh S., Geneva A.J., Morello T.D., Seluanov A, & Gorbunova V. (2014). SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and age. Nature communications, 5, 5011.
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3

Western Blotting for Chromatin Protein Analysis

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Western blotting was performed using the methods reported by Legartová et al. (2014) and Franek et al. (2016) [43 (link),6 (link)]. We used the following primary antibodies: anti-53BP1 (#ab21083, Abcam), anti-α-tubulin (#ab80779, Abcam), anti-MDC1 (#ab11169, Abcam), anti-MDC1 (#ab41951, Abcam), anti-phosphorylated histone H2AX (γH2AX; phospho S139; #ab2893, Abcam), anti-HP1β (#MAB3448, Merck), anti-H3 (#ab7091, Abcam), anti-H3K9me1 (#ab9045, Abcam), anti-H3K9me2 (#ab1220, Abcam), anti-H3K9me3 (#ab8898, Abcam), anti-histone H3K9ac (#06-942, Merck), anti-histone H4K20ac (#701778, ThermoFisher Scientific), anti-histone H4K20me2 (#A-4047-050, Epigentek, Lab Mark a.s.), anti-histone H4K20me3 (#A-4048-050, Epigentek, Lab Mark a.s.). As secondary antibodies, we used anti-rabbit IgG (#A-4914, Merck, Germany; dilution 1:2000), anti-mouse IgG (#A-9044, Merck; dilution 1:2000) and anti-mouse IgG1 (#sc-2060, Santa Cruz Biotechnology, Dallas, Texas, USA; dilution 1:1000).
Svobodová Kovaříková A., Legartová S., Krejčí J, & Bartová E. (2018). H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions. Aging (Albany NY), 10(10), 2585-2605.
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4

Histone H3 Immunoblotting Protocol

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Anti-histone H3 (1/10,000; ab1791, Abcam, Cambridge, UK), swine anti-rabbit IgG (1/2500; P0399; DAKO, Hamburg, Germany), anti-His6-peroxidase (1/500; 1965085001; Roche, Mannheim, Germany); anti-hHP1β (1:2000; MAB3448, Merck Millipore, Schwalbach, Germany). Uncropped scans of gels and blots are provided in Supplementary Fig. 12.
Hiragami-Hamada K., Soeroes S., Nikolov M., Wilkins B., Kreuz S., Chen C., De La Rosa-Velázquez I.A., Zenn H.M., Kost N., Pohl W., Chernev A., Schwarzer D., Jenuwein T., Lorincz M., Zimmermann B., Walla P.J., Neumann H., Baubec T., Urlaub H, & Fischle W. (2016). Dynamic and flexible H3K9me3 bridging via HP1β dimerization establishes a plastic state of condensed chromatin. Nature Communications, 7, 11310.
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