Sml1644

Manufactured by Merck Group

The SML1644 is a specialized laboratory equipment product from Merck Group. It is designed for precise and reliable measurements in research and scientific applications. The core function of this device is to provide accurate and consistent data collection, without further interpretation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Bafilomycin a1 bafa, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

2 protocols using sml1644

Autophagy Inhibitors Modulate Cell Responses

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24 h prior to in vitro experiment, cells were passaged and plated in 24-well plates at a density of 5 × 10⁴ cells per well. Specified cells were pre-treated with LPS (10 ng/ml; Sigma, L2630), 3 h prior to drug treatment. The following autophagy inhibitors were used: Bafilomycin A₁ (BafA) (20 nM; Sigma-Aldrich, B1793), 3-MA (100 μM; Sigma-Aldrich, M9281) and MRT68921 (10 µM; Sigma-Aldrich, SML1644). Treatment with the inhibitors was carried out for 6 h, following which all samples were lysed in Laemmli buffer and processed for western blotting. For all in vitro experiments, n = 3 replicates/group with 3 independent experiments performed.

Hegdekar N., Sarkar C., Bustos S., Ritzel R.M., Hanscom M., Ravishankar P., Philkana D., Wu J., Loane D.J, & Lipinski M.M. (2023). Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes. Autophagy, 19(7), 2026-2044.

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Autophagy Modulation in SIM-A9 Cells

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Treatments of SIM-A9 cell cultures included PIK3C3/ Vps34 inhibition by SAR405 (0.1, 1 and 10 µM, 4 h; 533063, Merck), ULK1 inhibition by MRT68921 (0.1, 1 and 10 µM, 4 h; SML1644, Sigma-Aldrich) and stimulation of SA through the induction of lysosomal damage by LLOMe (0.25 mM, 4 h; L7393, Sigma-Aldrich). BafA1 (0.1 µM, 4 h; J61835, Thermo Scientific Chemicals) was used along with SAR405 or MRT68921 to asses autophagy flux and validate inhibition of autophagy flux via LC3 Western Blotting according to the guidelines for the use and interpretation of assays for monitoring autophagy^{57 (link)}.

Hartmann J., Bajaj T., Otten J., Klengel C., Ebert T., Gellner A.K., Junglas E., Hafner K., Anderzhanova E.A., Tang F., Missig G., Rexrode L., Trussell D.T., Li K.X., Pöhlmann M.L., Mackert S., Geiger T.M., Heinz D.E., Lardenoije R., Dedic N., McCullough K.M., Próchnicki T., Rhomberg T., Martinelli S., Payton A., Robinson A.C., Stein V., Latz E., Carlezon WA J.r., Hausch F., Schmidt M.V., Murgatroyd C., Berretta S., Klengel T., Pantazopoulos H., Ressler K.J, & Gassen N.C. (2024). SKA2 regulated hyperactive secretory autophagy drives neuroinflammation-induced neurodegeneration. Nature Communications, 15, 2635.

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