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Alexa fluor 488 goat anti mouse a 11001

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Alexa Fluor 488 goat anti-mouse (A-11001) is a secondary antibody conjugate that binds to mouse primary antibodies. It is designed for use in immunofluorescence microscopy, flow cytometry, and other immunodetection applications. The Alexa Fluor 488 dye provides bright green fluorescence when excited at 488 nm.

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Lab products found in correlation

Axioskop fluorescent microscope, by Zeiss (1 mentions) Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Dmi6000b inverted microscope, by Leica (1 mentions) Acetylated tubulin, by Santa Cruz Biotechnology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti rabbit a 11008, by Thermo Fisher Scientific (1 mentions) Polyclonal horse radish peroxidase conjugated sheep anti mouse igg na931v, by GE Healthcare (1 mentions)

3 protocols using alexa fluor 488 goat anti mouse a 11001

1

Immunofluorescence Staining of FFPE Tissues

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Immunofluorescence was performed on unstained formalin-fixed paraffin-embedded slides. Slides were first deparaffinized through standard xylene wash followed by standard rehydration. Antigen retrieval was performed in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween-20, pH 6.0) at 95°−100°C for 10 minutes. Samples were blocked in 1% bovine serum albumin (BSA) in 1× phosphate-buffered saline (PBS) with 0.05% Tween-20. Serial antibody staining was performed with S-100 (AB941; Chemicon; 1:60) primary antibody and Alexa Fluor 546 goat anti-rabbit (A-11010; Life Technologies; 1:1000) secondary antibody, followed by neurofilament (sc-58561; Santa Cruz Biotechnologies; 1:60) primary antibody and Alexa Fluor 488 goat anti-mouse (A-11001; Life Technologies; 1:1000) secondary antibody. All antibody steps were diluted in 1× PBS and performed for 1 hour at room temperature. Nuclei were stained with DAPI at 1:1000 dilution of 1× PBS for 10 minutes. Slides were imaged on a Zeiss Axioskop fluorescent microscope with associated Zeiss AxioVision software.
Hsu M, & Stevenson F.F. (2014). Wallerian Degeneration and Recovery of Motor Nerves after Multiple Focused Cold Therapies. Muscle & Nerve, 51(2), 268-275.
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2

Immunofluorescence Staining of Cell Markers

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MDA-MB-231 cells were plated on glass coverslips and treated as described. Cells were fixed in 10% formalin, blocked in 5% BSA in TBS containing 0.1% Triton-X (Roche), incubated in 1:500 primary antibody (β-catenin, Cell Signaling Technologies; Cat. No. 9581S), (PARD3 (ab64646)/ Numb (ab14140)/ APT1 (ab91606)/ GFP (ab290)/ Caveolin (ab17052), Abcam), (DHHC20, Sigma; Cat. No. HPA014483), (Acetylated tubulin, Santa Cruz; Cat. No. sc23950), 1:1000), for 1-2 hours at room temperature, incubated in secondary antibody (Alexafluor 488 goat anti-mouse (A11001)/ Alexafluor 594 goat anti-rabbit (A11012), Life Technologies; 1:1000) for 1 hour at room temperature, and mounted in DAPI-mount (Southern Biotech; Cat. No. 0100-20). ESCs were cultured without LIF, MEK or GSK inhibitors for 24 hours before staining with 1:1000 anti-GFP (ab290) as described. Cells were imaged using the Leica DMI6000 B inverted microscope on 40X magnification and colonies were imaged on 20X magnification.
Stypulkowski E., Asangani I.A, & Witze E.S. (2018). The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell division. Science signaling, 11(511), eaam8705.
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3

Antibody Sources for Western Blotting

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Rabbit polyclonal antibody anti-light chain 3B (anti-LC3B; #2775) was from Cell Signaling (Beverly, MA, USA). Mouse monoclonal anti-SQSTM1/p62 (sc-28359) and rabbit polyclonal anti-nuclear factor kappa B (anti-NFκB) p65 (sc-109) antibody were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Mouse monoclonal anti-α Tubulin (DM1-A; CP06) was from EMD/Biosciences-Calbiochem (San Diego, CA, USA). Polyclonal horse radish peroxidase (HRP)-conjugated sheep anti-mouse IgG (NA931V) and polyclonal HRP-conjugated donkey anti-rabbit IgG (NA934V) were purchased from GE Healthcare (Malborough, MA, USA). Alexa Fluor®488 goat anti-rabbit (A11008) and Alexa Fluor®488 goat anti-mouse (A11001) were from Life Technologies Corporation (Grand Island, NY, USA).
Bermúdez V., Tenconi P.E., Giusto N.M, & Mateos M.V. (2019). Lipopolysaccharide-Induced Autophagy Mediates Retinal Pigment Epithelium Cells Survival. Modulation by the Phospholipase D Pathway. Frontiers in Cellular Neuroscience, 13, 154.
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