Human bladder cancer BIU-87 cells (a kind gift from Professor Xiaofeng Yang of the Department of Urology, our hospital, China) were cultured in RPMI-l640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 0.05 g/L streptomycin (North China Pharmaceutical Group Corp., Shijiazhuang, China), 0.05 g/L penicillin (North China Pharmaceutical Group Corp., Shijiazhuang, China), 0.8 g/L NaHCO3 (Tianjin DaMao Chemical Reagent Company), 3.6 g/L HEPES (Sangon Biotech Co. Ltd, Shanghai, China) and 10% fetal bovine serum (Hangzhou Sijiqing Biological Engineering Materials Co. Ltd, Hangzhou, China). The cells were incubated at 37 °C in a humidified environment containing 5% CO2 (NU-5500E incubator, Nuaire, Plymouth, MN, USA). For the experiments, cells in the logarithmic growth phase were digested into suspension with 0.25% trypsin (Sangon Biotech Co. Ltd, Shanghai, China).
Rpmi l640 medium
Manufactured by Thermo Fisher Scientific
Sourced in China
RPMI-1640 medium is a commonly used cell culture medium. It provides the necessary nutrients and components to support the growth and maintenance of a variety of cell types in vitro. The medium contains a balanced salt solution, amino acids, vitamins, and other essential elements required for cell proliferation and survival.
Automatically generated - may contain errors
2 protocols using rpmi l640 medium
1
Culturing Human Bladder Cancer BIU-87 Cells
2
In Vitro Cytotoxicity Evaluation of Compounds
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PCR kit was purchased from Thermo Fisher Scientific (China) Co., Ltd., Shanghai, China; RPMI-l640 medium from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA; 10% fetal bovine serum from Shanghai Harling Biotechnology Co., Ltd., Shanghai, China; Thiazolyl Blue (MTT) and dimethyl sulfoxide (DMSO) from Suzhou Lianxiong Chemical Technology Co., Ltd., Suzhou, China; 3.5% chloral hydrate from Qingdao Yulong Seaweed Co., Ltd., Qingdao, China; Olympus IX71 inverted fluorescence microscope from Beijing Presisi Instrument Co., Ltd., Beijing, China; YLS-5Q desktop super temperature control scald instrument from Beijing Ruiyisi Technology Co., Ltd., Beijing, China; super clean bench from Chongqing Pharmaceutical Co., Ltd. Keyihuabo Branch Company, Chongqing, China; ultra-low temperature refrigerator from Thermo Fisher Scientific, Inc. Operations were performed strictly in accordance with the respective instructions.
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