Htb 79

The effect of PPEs on cell proliferation was evaluated against three lines of human cancer cells in addition to normal human fibroblasts. Two cancer cell lines were of the male and female reproductive systems, and the third cell line was of the digestive system. These cells were prostate adenocarcinoma PC3 (ATCC® CRL‐1435), ovarian cancer cell line SKOV‐3 (ATCC® HTB‐79), and colorectal carcinoma HCT 116 (ATCC® CCL‐247). The fibroblasts were MRC‐5 (ATCC® CCD‐1064Sk).

Cell lines SW480 (Dukes’ type B, colorectal adenocarcinoma; ATCC^® CCL-228^™), HCT-116 (colorectal carcinoma; ATCC^® CCL-247^™), A2780 and A2780cis (ovarian carcinoma, Sigma), KB-3-1 (Human papillomavirus-related endocervical adenocarcinoma; HELA derivative, obtained from Dr. D. W. Shen, Bethesda, Maryland) VM47 (primary melanoma as described previously^{50 (link)}), H35 and H52 (primary glioblastoma as described previously^{51 (link)}) and Capan-1 (liver metastatic pancreatic cancer; ATCC^® HTB-79^™) were grown as a monolayer at 37 °C in a humified 5% CO₂ atmosphere. RPMI growth medium was supplemented with 10% fetal bovine serum (PAA, Linz, Austria). Cell cultures were periodically checked for Mycoplasma contamination. All media were supplemented with 2 mM MES buffer (pH 6, 1% vol) or H₂O (1% vol).