A 10-mL blood sample was collected by venipuncture by a registered nurse. For measurement of HbA1c, blood samples were collected in ethylenediaminetetraacetic acid tubes. Then HbA1C was measured in whole blood using the immune-inhibition test for the quantitative determination of glycosylated hemoglobin (Beckman Coulter AU analyzers). For the measurement of CRP and IL-6, blood was collected in Z-Clot activator tubes. Blood was allowed to clot before centrifugation for 15 mins at 1000×g. Aliquots of serum were stored at ≤ −22°C in sterile small tubes prior to biochemical assay. CRP and IL-6 concentrations were analyzed within a month after collection of blood. The immuno-turbidimetric test was used to determine CRP levels (Beckman Coulter AU analyzers). IL-6 was measured using a human immunoassay kit from R&D SYSTEMS through sandwich-type enzyme-linked immunosorbent assay. An Absorbance Microplate Reader was used to measure the optical density of IL-6 (BioTek ELx800).
Au analyzer
Manufactured by Beckman Coulter
Sourced in United States
The AU analyzer is a compact, automated clinical chemistry analyzer designed for routine clinical chemistry testing. It provides accurate and reliable results for a wide range of common biochemical assays used in medical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Elx800, by Agilent Technologies (1 mentions)
Au system crp latex reagent, by Beckman Coulter (1 mentions)
Defibrinated sheep blood, by Thermo Fisher Scientific (1 mentions)
Coulter lh 750 hematology analyzer, by Beckman Coulter (1 mentions)
Quantichrom heme assay kit, by BioAssay Systems (1 mentions)
Tnf alpha elisa, by DRG International (1 mentions)
Nucleospin blood kit, by Macherey-Nagel (1 mentions)
Erenna platform, by Merck Group (1 mentions)
Advia centaur vitamin d total assay, by Siemens (1 mentions)
704 analyzer, by Roche (1 mentions)
Ion exchange high performance liquid chromatography, by Bio-Rad (1 mentions)
Realtime hcv, by Abbott (1 mentions)
Advia centaur xp system, by Siemens (1 mentions)
Elecsys system, by Roche (1 mentions)
31 protocols using au analyzer
1
Quantification of HbA1c, CRP, and IL-6 in Blood
2
Fasted Blood Biomarker Assessment
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Fasting (≥12 h) blood was obtained from the antecubital vein by trained phlebotomists. Blood glucose, insulin, triglycerides, HDL-C, LDL and total cholesterol concentrations were determined by colorimetric assays on Beckman Coulter AU Analyzers [57 (link),58 ]. Homeostasis model assessment (HOMA-IR) was used to estimate insulin resistance using the validated equation: Fasting Plasma Insulin × Fasting Plasma Glucose (mmol/L)/22.5 [59 (link)]. Progesterone, estradiol and testosterone were determined by chemiluminescence on Beckman Coulter DXi Analyzers [60 (link),61 (link)]. Serum high sensitivity C-Reactive Protein (hs-CRP) levels were quantitatively determined by the Beckman Coulter AU System CRP Latex reagent on Beckman Coulter AU Analyzers.
3
Glycosylated Hemoglobin and High-Sensitivity CRP Analysis
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A 10-ml sample of venous blood was collected from each patient by a registered nurse. The blood samples were collected in ethylenediaminetetraacetic acid tubes, and HbA1c was measured in whole blood using the immuno-inhibition test for the quantitative determination of glycosylated hemoglobin (Beckman Coulter AU analyzers). Blood samples were collected in Z-Clot activator tubes and allowed to clot before centrifugation for 15 min. Aliquots of serum were stored at ≤−22°C in sterile small tubes before biochemical assay. Immuno-turbidimetric test was used to determine hs-CRP levels (Beckman Coulter AU analyzers).
4
Circulating Iron and Oxidative Stress Markers
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Markers of circulating iron and oxidative stress were analyzed in the blood as previously described,39 (link) using the methods listed as follows. Whole-blood catalase was measured colorimetrically using commercially available kits (Cayman Chemical Company, Scottsdale, AZ, USA). Whole-blood glutathione peroxidase was measured on an Ace Alera autoanalyzer using the Randox GPX method and reagents (Randox, Crumlin, UK). Serum ferritin was measured using a commercially available rat ferritin ELISA assay (Alpco Immunoassay, Salem, NH, USA). Plasma iron was measured using a Beckman Coulter AU analyzer. Plasma heme was measured using the Quantichrom Heme Assay Kit, from BioAssay Systems (Hayward, CA, USA). Serum C-reactive protein was measured using the Rat C-reactive Protein ELISA kit from EIAab (Wuhan, China). Serum total antioxidant capacity was measured using Randox reagents on a Beckman Coulter AU analyzer, with a blank measurement being taken of each sample before reagents were added. This allows subtraction of any contribution from urate, ascorbate, or albumin. Hematocrit and transferrin were measured on a Coulter LH750 hematology analyzer (Beckman Coulter, Brea, CA, USA).
5
Comprehensive Serum and Urinary Evaluation
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Serum calcium assay was done using the modified ortho-cresolphthalein complexone method on the Beckman Coulter AU analyzer; the reference range for normal serum calcium in our laboratory is 8.5 to 10.5 mg/ dl. Serum phosphate was assessed using modified phosphomolybdate method on the Beckman Coulter AU analyzer; the reference range for normal serum phosphate in our laboratory is 2.5 to 4.5 mg/dl. Intact parathyroid hormone (iPTH) was measured by electrochemiluminescence (ECLIA) sandwich assay (Elecsys system, Roche Diagnostics), with the normal reference range being 15 to 65 pg/ ml. 24-hour urinary calcium was measured by a modified ortho-cresolphthalein complexone method; values above 4 mg/kg/24 hours were considered as evidence of hypercalciuria. Urine was collected in a container containing 10-20 ml of 6 N (M) hydrochloric acid (HCl). 25 hydroxy vitamin D [25(OH)D] was measured by competitive immunoassay (ADVIA Centaur XP system Siemens healthcare diagnostics), with a value below 20 ng/ml being considered as evidence of deficiency.
6
Urinary Sodium and Potassium Measurement
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Urinary sodium and potassium were measured using the ion-selective electrode and atomic absorption spectroscopy methods on Beckman Coulter AU Analyzers. Laboratory values were normalized to 24 h based on each participant’s total collection time. For example, if the duration of collection was 23 h, we multiplied the sodium value by 24/23. Results are presented for normalized values of sodium and potassium (mg/d) and the sodium-to-potassium ratio that was calculated by dividing the normalized sodium value (mg) by the normalized potassium value (mg).
7
Quantifying Oxidized LDL and Lipid Profiles
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OxLDL concentrations of serum samples were analysed using the Human oxLDL/MDA Adduct Elisa. (Bensheim, Germany). OxLDL concentrations were determined at T0 and T2 (+ 7 d). LDL and HDL concentration were determined using the Beckman Coulter AU analyzers (Inc., 250 S. Kraemer Blvd. Brea, CA 92821, USA). LDL and HDL concentrations were determined at T0 and T2 (+ 7 d).
8
Measuring hsCRP as Inflammation Biomarker
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The nonspecific acute phase reactant, hsCRP was measured as the most reliable biomarker of on-going inflammation implicated in pathophysiology of depression and its response to treatment (Haroon et al., 2018 (link), Miller and Raison, 2016 (link)). Recent research has confirmed that hsCRP is a clinically valuable inflammatory marker, both peripheral and central, and can serve as a proxy for cytokines and other inflammatory mediators, which are more difficult to measure in the clinic (Felger et al., 2018 (link), Miller et al., 2017 (link)). The CRP analysis has been described in detail in (Chamberlain et al., 2019 (link)) but briefly, the blood samples were collected in clot activator containing tubes for measurements of hsCRP. The samples were allowed to coagulate for 30–60 min then centrifuged at 1600 Relative Centrifugal Force (RCF) for 15 min. The serum samples were separated and transported to a central laboratory (Q2 Solutions) where analysed on the day of receipt. Samples were exposed to anti-CRP-antibodies on latex particles, and the increase in light absorption due to complex formation was used to quantify hsCRP levels, using Turbidimetry on Beckman Coulter AU analyzers. Inter and intra-assay co-efficient of variations were <10%. The Clinical Reporting Range (CRR) for the hsCRP assay was 0.2–9999.9 mg/L.
9
Standardized CRP Measurement Protocol
10
Urine Glycosaminoglycan Analysis Protocol
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