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Sirtinol

Manufactured by Cayman Chemical
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Sirtinol is a chemical compound used in laboratory research. It functions as a selective inhibitor of the sirtuin family of proteins, specifically targeting SIRT1. Sirtinol is utilized by researchers to study the biological roles and mechanisms of sirtuin enzymes.

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Lab products found in correlation

Ms 275, by Cayman Chemical (2 mentions) Trichostatin a, by Merck Group (2 mentions) Vorinostat, by Cayman Chemical (2 mentions) Tubacin, by Cayman Chemical (2 mentions) Hdac activity fluorometric assay kit, by Abcam (2 mentions) Black 96 well plate, by Thermo Fisher Scientific (2 mentions) Synergy h1 hybrid reader, by Agilent Technologies (2 mentions) Sirtinol, by Merck Group (1 mentions) Ici 182 780, by Merck Group (1 mentions) Compound c, by Merck Group (1 mentions) Ici 182 780, by Cayman Chemical (1 mentions) Compound c, by Cayman Chemical (1 mentions) Panobinostat, by Cayman Chemical (1 mentions) Sodium phenylbutyrate, by Merck Group (1 mentions) Tenovin 1, by Bio-Techne (1 mentions) Paclitaxel, by Cayman Chemical (1 mentions) Pargyline, by Merck Group (1 mentions) Etoposide, by Cayman Chemical (1 mentions) Salinomycin, by Cayman Chemical (1 mentions) Nocodazole, by Merck Group (1 mentions)

5 protocols using sirtinol

1

Estrogen Receptor Signaling Inhibition

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Cells were treated with 10 nmol/L E2 in phenol red‐free DMEM supplemented with 2% charcoal‐stripped fetal bovine serum. Control cells were exposed to the same volume of medium without E2. For the inhibition experiments performed using ICI 182780 (Sigma), sirtinol (Sigma), and Compound C (Sigma), new DMEM supplemented with 2% charcoal‐stripped fetal bovine serum was added and maintained for 30 minutes, 1, 3, 6, and 24 hours before the addition of the inhibitors: ICI 182780 (1 μmol/L), sirtinol (50 μmol/L), Compound C (10 μmol/L), or G15 (2 μmol/L; Cayman).
Sasaki Y., Ikeda Y., Uchikado Y., Akasaki Y., Sadoshima J, & Ohishi M. (2021). Estrogen Plays a Crucial Role in Rab9‐Dependent Mitochondrial Autophagy, Delaying Arterial Senescence. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 10(7), e019310.
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2

HDAC Activity Fluorometric Assay

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The HDAC Activity Fluorometric Assay Kit (BioVision) was used to measure deacetylation activity of in vitro translated or recombinant proteins. The test samples were incubated with the provided fluorogenic substrate, Boc-Lys(Ac)-AMC [complete name: Nα-(t-butoxycarbonyl)-Nω-acetyl-L-lysine 7-amido-4-methylcoumarin] in 1×HDAC Assay Buffer at 37°C for 2.5 hrs, and then the reaction was stopped by adding the Lysine Developer and further incubated at 37°C for 30 minutes. The produced fluorescence was measured in 96-well black plate (Thermo Scientific) using the Synergy H1 Hybrid reader (BioTek Instruments) with excitation at 360 nm and emission at 460 nm. In some assays, the following HDAC inhibitors were used at concentrations that were 20–300 fold of their reported IC50, Vorinostat, MS-275 (also known as Entinostat), Tubacin, Sirtinol (all from Cayman Chemical) and Trichostatin A (Sigma-Aldrich).
Xing S., Li F., Zeng Z., Zhao Y., Yu S., Shan Q., Li Y., Phillips F.C., Maina P.K., Qi H.H., Liu C., Zhu J., Pope R.M., Musselman C.A., Zeng C., Peng W, & “Howard” Xue H.H. (2016). Tcf1 and Lef1 transcription factors establish CD8+ T cell identity through intrinsic HDAC activity. Nature immunology, 17(6), 695-703.
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3

Resuspension and Dilution of Common Histone Deacetylase Inhibitors

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Drugs for this study were purchased as follows: tenovin-1 was purchased from Tocris Bioscience (catalog no. 336510), sirtinol from Cayman Chemical (catalog no. 10523), sodium phenylbutyrate from Sigma (catalog no. P21005), panobinostat from Cayman Chemical (catalog no. 13280), selisistat/EX527 from Cayman Chemical (catalog no. 10009798), and AGK2 from Cayman Chemical (catalog no. 13145). Drugs were resuspended in dimethyl sulfoxide (DMSO), except for NaPB, which was resuspended in water. Drugs were diluted in culture media for use.
Hackett B.A., Dittmar M., Segrist E., Pittenger N., To J., Griesman T., Gordesky-Gold B., Schultz D.C, & Cherry S. (2019). Sirtuin Inhibitors Are Broadly Antiviral against Arboviruses. mBio, 10(4), e01446-19.
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4

Screening Anti-Cancer Stem Cell Agents

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We cultured HCT116, HT29, and DLD-1 cells under standard culture conditions in RPMI-1640 media (Welgene) containing 10% FBS (Atlas Biologicals) and 1 × penicillin-streptomycin (Corning). For the drug treatments, we dissolved JIB-04 (Cayman) and salinomycin (Cayman) in DMSO and diluted the solutions to the appropriate concentrations for the experiments. The following chemicals were used for the primary screening of anti-CSC agents: IOX1 (Cayman), SAHA (Cayman), trichostatin (TSA) (Cayman), EPZ5676 (APEXBIO), eosin Y disodium trihydrate(AMI-5) (Santa Cruz), 2-PCPA(Cayman), sirtinol (Cayman), pargyline (Sigma-Aldrich), paclitaxel (Cayman) 5-FU (Cayman), etoposide (Cayman), nocodazole (Sigma-Aldrich), calmidazolium chloride (Tocris Bioscience), beta-lapachone (Cayman).
Kim M.S., Cho H.I., Yoon H.J., Ahn Y.H., Park E.J., Jin Y.H, & Jang Y.K. (2018). JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway. Scientific Reports, 8, 6611.
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5

HDAC Activity Fluorometric Assay

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The HDAC Activity Fluorometric Assay Kit (BioVision) was used to measure deacetylation activity of in vitro translated or recombinant proteins. The test samples were incubated with the provided fluorogenic substrate, Boc-Lys(Ac)-AMC [complete name: Nα-(t-butoxycarbonyl)-Nω-acetyl-L-lysine 7-amido-4-methylcoumarin] in 1×HDAC Assay Buffer at 37°C for 2.5 hrs, and then the reaction was stopped by adding the Lysine Developer and further incubated at 37°C for 30 minutes. The produced fluorescence was measured in 96-well black plate (Thermo Scientific) using the Synergy H1 Hybrid reader (BioTek Instruments) with excitation at 360 nm and emission at 460 nm. In some assays, the following HDAC inhibitors were used at concentrations that were 20–300 fold of their reported IC50, Vorinostat, MS-275 (also known as Entinostat), Tubacin, Sirtinol (all from Cayman Chemical) and Trichostatin A (Sigma-Aldrich).
Xing S., Li F., Zeng Z., Zhao Y., Yu S., Shan Q., Li Y., Phillips F.C., Maina P.K., Qi H.H., Liu C., Zhu J., Pope R.M., Musselman C.A., Zeng C., Peng W, & “Howard” Xue H.H. (2016). Tcf1 and Lef1 transcription factors establish CD8+ T cell identity through intrinsic HDAC activity. Nature immunology, 17(6), 695-703.
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