Atellica im analyzer

Manufactured by Siemens

Sourced in Germany

The Atellica IM Analyzer is a laboratory equipment product developed by Siemens. It is designed to perform automated immunoassay testing for a variety of analytes. The core function of the Atellica IM Analyzer is to provide accurate and reliable results for clinical diagnostic testing.

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Atellica (20 citations) Atellica IM (8 citations) Atellica Analyzer (4 citations) Atellica IM 1300 analyzer (3 citations)

13 protocols using atellica im analyzer

Testicular Biomarkers in COVID-19

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The enzymatic activity of MPO and NAG and the total concentration of testosterone and angiotensin II were determined in human testis homogenates. For this purpose, 50 mg of snap-frozen testis from deceased COVID-19 patients and controls were homogenized in 450 μL cold PBS supplemented with a protease inhibitor cocktail (Cat n° S8830, Sigma-Aldrich). After three freeze/thaw cycles in a liquid nitrogen/water bath (37 °C), the samples were centrifugated (14.000 g, 10 min, 4 °C), and the supernatants were collected.
For MPO and NAG measurements, 100 μL of tissue homogenates were mixed 1:1 in MPO buffer assay (0,1M Na3PO4, 0.1% [w/v] HETAB, pH 5.0) or NAG buffer assay (0.2M citric acid, 0.2M Na2HPO4, pH 4.5), respectively, just prior the freeze/thaw step. The activity of MPO and NAG, which is an indirect estimation of the abundance of neutrophils and macrophages, was measured in a colorimetric enzymatic assay. Intratesticular testosterone levels were determined in testis homogenates using a chemiluminometric immunoassay run on the Atellica IM Analyzer (Siemens Healthcare Diagnostics). The concentration of Angiotensin II was measured by ELISA, according to the procedures supplied by the manufacturer (MyBioSource, San Diego, CA, USA). The kit applied the sandwich ELISA technique. The sensitivity of the assay was 12 pg/mL.

Costa G.M., Lacerda S.M., Figueiredo A.F., Wnuk N.T., Brener M.R., Andrade L.M., Campolina-Silva G.H., Kauffmann-Zeh A., Pacifico L.G., Versiani A.F., Antunes M.M., Souza F.R., Cassali G.D., Caldeira-Brant A.L., Chiarini-Garcia H., de Souza F.G., Costa V.V., da Fonseca F.G., Nogueira M.L., Campos G.R., Kangussu L.M., Martins E.M., Antonio L.M., Bittar C., Rahal P., Aguiar R.S., Mendes B.P., Procópio M.S., Furtado T.P., Guimaraes Y.L., Menezes G.B., Martinez-Marchal A., Orwig K.E., Brieño-Enríquez M, & Furtado M.H. (2023). High SARS-CoV-2 tropism and activation of immune cells in the testes of non-vaccinated deceased COVID-19 patients. BMC Biology, 21, 36.

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SARS-CoV-2 IgG Antibody Quantification

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Buffy coats from anonymous healthy donors were provided by the Blood Transfusion Center of the Red Cross (Mechelen, Belgium). A total of 3 mL was transferred to an SST™ II Advance Vacutainer^® Blood collection tube (BD Diagnostics, Plymouth, UK), and serum was isolated according to the manufacturer’s instructions. COVID-19 serology was tested using the Atellica^® IM SARS-CoV-2 IgG (sCOVG) assay (Siemens Healthineers, Beersel, Belgium). In short, SARS-CoV-2-specific IgG antibodies were captured using streptavidin-coated microparticles with biotinylated SARS-CoV-2 recombinant antigens. After the addition of an acridinium-ester-labeled anti-human IgG mouse antibody, the amount of SARS-CoV-2-IgG antibody could be determined by measuring on the Atellica IM Analyzer (Siemens Healthineers).

Van Delen M., Janssens I., Dams A., Roosens L., Ogunjimi B., Berneman Z.N., Derdelinckx J, & Cools N. (2022). Tolerogenic Dendritic Cells Induce Apoptosis-Independent T Cell Hyporesponsiveness of SARS-CoV-2-Specific T Cells in an Antigen-Specific Manner. International Journal of Molecular Sciences, 23(23), 15201.

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Mixed-Meal Tolerance Test for C-Peptide

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A mixed-meal tolerance test was performed between 8:30 am and 10:30 am following an overnight fast, with no injection of bolus insulin in the preceding 6 hours. Boost (formerly Sustacal, Mead Johnson), at a dose of 6 mL/kg (maximum 360 mL), was ingested in less than 10 minutes. Blood draws were obtained for baseline glucose and C-peptide, and at 30 minutes and 90 minutes for post–mixed-meal C-peptide and glucose estimations [14 (link)]. Glucose and C-peptide were analyzed by the UMass Memorial Medical Center Biochemistry laboratory. SCP concentrations were analyzed by enzyme-linked immunosorbent assay using Quest Immunoassays on an Atellica IM Analyzer (Siemens) with less than 12% intra-assay and interassay coefficients of variability.

Nwosu B.U., Parajuli S., Jasmin G., Fleshman J., Sharma R.B., Alonso L.C., Lee A.F, & Barton B.A. (2021). Ergocalciferol in New-onset Type 1 Diabetes: A Randomized Controlled Trial. Journal of the Endocrine Society, 6(1), bvab179.

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Seroprevalence of SARS-CoV-2 IgG Antibodies

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To assess IgGs against SARS-CoV-2, we first qualitatively screened total antibodies with a chemiluminescent immunoassay, the Atellica^® IM SARS-CoV-2 Total assay, using an Atellica^® IM Analyzer (Siemens Healthcare, Erlangen, Germany). This assay is directed toward the receptor-binding domain (RBD) in the S1 subunit of the SARS-CoV spike (S) protein. All positive samples were re-tested with a confirmatory ELISA assay, SARS-CoV-2 (Euroimmun, Lübeck, Germany) already in use at our center for diagnostic routines. This assay provides semiquantitative in vitro determination of immunoglobulins classes IgG, using the S1 domain of the Spike protein, including RBD. Sensitivity and specificity for Atellica^® IM SARS-CoV-2 Total assays were both 95% and for SARS-CoV-2 Euroimmun ELISA they were 90.3% and 98.5%, respectively. All plasma samples that were confirmed positive for SARS-CoV-2 IgG using the Euroimmunn ELISA test were considered ‘truly’ positive. Based on the random selection design, testing samples from the same subject at different times (months) were considered in the study.
We estimated an overall seroprevalence and an additional month-by-month seroprevalence as the number of ‘truly’ positive tests/total patients. Seroprevalence was reported as rate and 95% confidence intervals (CI).

Lombardi F., Ricci R., Belmonti S., Fabbiani M., Borghetti A., Baldin G., Ciccullo A., Tamburrini E., Visconti E., Sanguinetti M, & Di Giambenedetto S. (2021). Seroprevalence of SARS-CoV-2 Antibodies in HIV-Infected Patients in Rome, Italy during the COVID-19 Outbreak. Diagnostics, 11(7), 1154.

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Ferritin Quantification Using Chemiluminometric Assay

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Ferritin quantification analysis was carried out by making use of a two-point sandwich immunoassay by direct chemiluminometric technology, which uses constant amounts of two anti-ferritin antibodies, using an Atellica™ IM Analyzer (Siemens Healthineers, Erlangen, Germany) [41 (link)].

Marrero A.D., Quesada A.R., Martínez-Poveda B., Medina M.Á, & Cárdenas C. (2023). A Proteomic Study of the Bioactivity of Annona muricata Leaf Extracts in HT-1080 Fibrosarcoma Cells. International Journal of Molecular Sciences, 24(15), 12021.

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Biochemical Markers in Metabolic Evaluation

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7600-120 automatic biochemical instrument (Hitachi, Tokyo, Japan) was used to detect fasting blood glucose (FPG), serum creatinine (SCr), blood urea nitrogen (BUN), and UCr. Hemoglobin A1c (HbAlc) was determined by high-pressure liquid chromatography (Primus Ultra, Ireland), whereas fasting insulin (Fins), fasting CP (FCP), 24-h UCP, and morning urine UCP were measured by chemiluminescence [The intra- and inter-assay coefficients of variability were 1.5% and 3.5%%, respectively] on an Atellica IM analyzer (Siemens Healthcare Diagnostics, Shanghai). The lower limit of the CP assay was 0.03 ng/ml, and for this analysis, all CP concentrations<0.03 ng/ml were recorded as 0.03 ng/ml. The upper limit was 30 ng/ml, and all CP concentrations>30 ng/ml were recorded as 30 ng/ml.

Zhou W., Li J., Yuan X., Wang W., Zhou H., Zhang H, & Ye S. (2022). Application of urine C-peptide creatinine ratio in type 2 diabetic patients with different levels of renal function. Frontiers in Endocrinology, 13, 1052794.

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Procalcitonin Measurement: Atellica IM Analyzer

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PCT was measured with Atellica IM BRAHMS Procalcitonin (PCT) reagent on an Atellica IM analyzer (Siemens Healthcare Diagnostics Inc).
PCT reference values were established as < 0.05 μg/L. Within-laboratory precision is determined as < 7% CV for samples from 0.05 to 2.0 μg/L and < 5% CV for samples > 2.0 μg/L.

Hessels L., Speksnijder E., Paternotte N., van Huisstede A., Thijs W., Scheer M., van der Steen-Dieperink M., Knarren L., van Den Bergh J.P., Winckers K., Henry R., Simsek S, & Boersma W.G. (2023). Procalcitonin-Guided Antibiotic Prescription in Patients With COVID-19: A Multicenter Observational Cohort Study. Chest.

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Antibody Detection and Neutralization Assays

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Anti-N protein IgG antibodies were tested by qualitative ARCHITECT chemiluminescence microparticle immunoassay (Abbott Laboratories, USA). Anti-S1 RBD antibodies were tested using SARS-CoV-2 Total antibody test on Atellica IM analyzer (Siemens, Germany). Neutralizing antibodies were tested by SARS-CoV-2 Surrogate Virus Neutralization test (GenScript USA, Inc).

Shastri J., Parikh S., Agrawal S., Chatterjee N., Pathak M., Chaudhary S., Sharma C., Kanakan A., A V., Srinivasa Vasudevan J., Maurya R., Fatihi S., Thukral L., Agrawal A., Pinto L., Pandey R, & Sunil S. (2021). Clinical, Serological, Whole Genome Sequence Analyses to Confirm SARS-CoV-2 Reinfection in Patients From Mumbai, India. Frontiers in Medicine, 8, 631769.

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Quantifying Serum Cardiac Troponins

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Venous blood samples were collected for serum analysis using 3.5 mL serum tubes (S-Monovette, 7.5 mL, Sarstedt, Nümbrecht, Germany). To quantitatively measure serum high-sensitivity cardiac troponin T (hs-cTnT), we utilized the cobas Elecsys Troponin T hs STAT assay from Roche Diagnostics GmbH, Mannheim, Germany, following the manufacturer’s instructions. The hs-cTnT assay has a limit of blank of 2.14 ng/L, a limit of detection of 5 ng/L, and a 99th percentile cutoff point for myocardial damage at 14 ng/L. The coefficient of variation (CV) for this assay is 7% at the cutoff level.
For the quantitative measurement of serum high-sensitivity troponin I (hs-cTnI), we employed the Atellica IM Analyzer (Siemens Healthineers AG, Erlangen, Germany) along with the High-Sensitivity Troponin I (TnIH) assay. This hs-cTnI assay features a limit of blank of 3 ng/L, a limit of detection of 5 ng/L, and a 99th percentile myocardial damage cutoff point of 45.43 ng/L. The coefficient of variation (CV) is ≤12% for samples with 9–20 pg/mL (ng/L) and ≤10% for samples >20 pg/mL (ng/L). The manufacturer’s upper standard range for this assay is 45 ng/mL.

Wuestenfeld J.C., Kastner T., Hesse J., Fesseler L., Frohberg F., Rossbach C, & Wolfarth B. (2024). Differences in Troponin I and Troponin T Release in High-Performance Athletes Outside of Competition. International Journal of Molecular Sciences, 25(2), 1062.

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Standardized Blood Sample Handling for Hormonal Analysis

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Details on sampling and handling of blood samples for analysis of counterregulatory hormones are described elsewhere.⁷ For measurement of FFA, whole blood was drawn into tubes containing serum clot activator and kept for at least 20 minutes at room temperature and stored at −80°C before being analysed using an enzymatic colorimetric method (Cobas 8000; Roche Diagnostics, Basel, CH). For measurement of C‐peptide, blood was drawn into a lithium‐heparin tube, placed on ice until centrifugation and stored at −20°C before being analysed using a sandwich electrochemiluminescence immunoassay (Atellica IM Analyzer; Siemens Healthineers, Erlangen, DE). All samples were centrifuged at 4°C and approximately 2900 g for 15 minutes and stored until batch analyses could be performed.

Andersen A., Jørgensen P.G., Bagger J.I., Baldassarre M.P., Christensen M.B., Pedersen‐Bjergaard U., Lindhardt T.B., Gislason G., Knop F.K, & Vilsbøll T. (2022). Acute changes in plasma glucose increases left ventricular systolic function in insulin‐treated patients with type 2 diabetes and controls. Diabetes, Obesity & Metabolism, 24(6), 1123-1131.

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