Rabbit monoclonal anti mmp7 antibody

Immunohistochemistry analyses were performed on 5-μm, formalin-fixed, paraffin-embedded slides from tumors arising in the mutant mice. The following antibodies were used: rabbit polyclonal anti-E-cadherin antibody (1:200; Cell Signaling, #3195, Danvers, MA), rabbit polyclonal anti-Ki-67 antibody (1:200; Abcam, ab15580, Cambridge, UK), mouse monoclonal anti-β-catenin antibody (1:200; BD Transduction Laboratories, 610154, San Diego, CA), rabbit monoclonal anti-vimentin antibody (1:100; Cell Signaling, #5741), and rabbit monoclonal anti-MMP7 antibody (1:100; Cell Signaling, #3801). The invasive front of the tumor was defined as the microscopic interface between normal tissue of the host mouse and the tumor mass invading the submucosa or deeper regions of the stomach [20 (link)]. Differences in the percentage of positive immunostaining between the various genotypes were evaluated using the Student t-test.