The ROSA26-eGFP-DTA (ref. 43 (link)), the ROSA26-stop-EYFP (ref. 44 (link)), the Rag1 knockout14 (link) (Rag1−/−) and the 2D2 transgenic12 (link) mice were purchased from the Jackson Laboratory. The ROSA26-eGFP-DTA and the ROSA26-stop-YFP mice were bred to the Plp1-CreERT transgenic mice9 (link) to generate the Plp1-CreERT;ROSA26-eGFP-DTA (DTA) and Plp1-CreERT;ROSA26-stop-EYFP (reporter) mice, as previously described8 (link). All mouse studies were conducted in compliance with The University of Chicago’s and Northwestern University’s Animal Care and Use Committee (IACUC) guidelines.
Rosa26 egfp dta
Manufactured by Jackson ImmunoResearch
Sourced in United States
ROSA26-eGFP-DTA is a research tool that contains a transgene cassette designed for conditional expression of enhanced green fluorescent protein (eGFP) and diphtheria toxin A (DTA) in cells. The construct is designed to be integrated into the mouse ROSA26 locus, allowing for ubiquitous expression of the transgene upon Cre-mediated recombination.
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4 protocols using rosa26 egfp dta
1
Transgenic Mouse Models for Cell-Specific Labeling
2
Mouse Strains for Genetic Studies
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The following mouse strains were obtained from the Jackson Laboratory: Gli1‐LacZ (JAX# 008211), ROSA26‐eGFP‐DTA (JAX# 006331), Gli1‐CreERT2 (JAX# 007913) and Yapflox (JAX# 027929). All mice were housed in a pathogen‐free condition, maintained on the standard 12‐hour light‐dark cycle. Offspring were genotyped by PCR according to the primer sequences provided by the Jackson Laboratory, and mice were used for experiments regardless of sex at the age of 10‐12 weeks. All animal experiments were performed following the guidelines of the Intramural Animal Use and Care Committee of the Fourth Military Medical University (license number: 2018‐kq‐014).
3
Genetic Mouse Lines for Cardiac Research
4
Genetic Lineage Tracing of CD34+ Cells
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All animal experiments and protocols were performed in accordance with the National Institutes of Health’s Guide for the Care and Use of Laboratory. Animals were approved by the Research Ethics Committees of the First Affiliated Hospital of Zhejiang University. In this study, we used the following mouse strains: CD34-CreERT2 knock-in mice (C57BL/6 background) and Rosa26-tdTomato mice (B6.Cg-Gt(ROSA)26Sortm9(CAG−tdTomato)Hze/J)(JAX: 007,909) were purchased from Shanghai Biomodel Organism Co., Ltd. Rosa26- eGFP-DTA (JAX: 006,331) mice were purchased from the Jackson Laboratory, USA. CD34-CreERT2; Rosa26-tdTomato and CD34-CreERT2; Rosa26-tdTomato -DTA mice were constructed as described in the previous paper [25 (link)].
Mice were maintained at temperature (22 ± 1 °C) and humidity (65–70%) controlled room, with a 12-h light and 12-h dark cycle and allowed free access to chow and water. All mice generated or purchased were housed for at least one week before use.
Mice were maintained at temperature (22 ± 1 °C) and humidity (65–70%) controlled room, with a 12-h light and 12-h dark cycle and allowed free access to chow and water. All mice generated or purchased were housed for at least one week before use.
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