Male ICR mice (about 20 g) were obtained from Vital River Laboratory Animal Technology Co., Ltd, Shanghai. All mice were maintained and fed as described previously [17 (link), 24 (link)]. LP-cs was added to the drinking water at 1:20, and 8–9 ml/mouse was consumed daily. During the two weeks of the experiment, the mice were maintained on the LP-cs treatment. Animals were given a 6 g/kg dose of ethanol via gavage and maintained fasting overnight with free access to drinking water containing LP-cs. All animals were anesthetized after 6 h of the experiment, and blood and tissue samples were collected for assays as described previously [17 (link)].
Male icr mice
Manufactured by Charles River Laboratories
Sourced in China, Japan
Male ICR mice are a widely used laboratory mouse strain. They are outbred, albino mice that are characterized by their general vigor and adaptability. These mice are commonly used in a variety of research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions)
Male balb c nude mice, by Charles River Laboratories (1 mentions)
Standard chow, by Research Diets (1 mentions)
Male kunming mice, by Charles River Laboratories (1 mentions)
Folic acid, by Merck Group (1 mentions)
Ht22 cells, by iCell Bioscience (1 mentions)
Sparague dawley rats, by Charles River Laboratories (1 mentions)
Male sd rats, by Charles River Laboratories (1 mentions)
Male c57bl 6 mice, by Beijing Huafukang Biotechnology (1 mentions)
Fs30d, by Thermo Fisher Scientific (1 mentions)
Avanti mini extruder, by Avanti Polar Lipids (1 mentions)
Female icr mice, by Charles River Laboratories (1 mentions)
Wild type icr mice, by Charles River Laboratories (1 mentions)
Isoflurane anesthesia, by Baxter (1 mentions)
Pregnant sprague dawley rats, by Charles River Laboratories (1 mentions)
Standard laboratory chow, by CLEA Japan (1 mentions)
Crf 1, by Oriental Yeast (1 mentions)
Roswell park memorial institute 1640 medium, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Sanyo (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
60 protocols using male icr mice
1
Ethanol-Induced Liver Injury in Mice
2
ICR and BALB/c Nude Mice Study
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Male ICR mice (18–22 g) and male BALB/c nude mice (18–22 g) were purchased from Vital River Co. (Beijing, People’s Republic of China), and kept under specific pathogen-free condition for 1 week before the study, with free access to standard food and water. All studies in mice were performed in accordance with guidelines approved by the Ethics Committee of the Chinese Academy of Medical Sciences and Peking Union Medical College. The committee has approved the experiments.
3
Husbandry and Housing of ICR Mice
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Male ICR mice weighing 18-22 g were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The animals were housed with controlled temperatures (25 ± 2°C), constant humidity (50 ± 10%) and a 12-h light/12-h dark cycle. Both standard laboratory food and water were available to the mice ad libitum. All the mice were housed in standard cages with 6 mice per cage, which were cleaned every day. This study was approved by the animal ethics committee of Chengdu University of Traditional Chinese Medicine, China. All procedures were carried out in accordance with the Guiding Principles for the Care and Use of Laboratory Animals of China.
4
Mice Housing and Ethical Approval
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Male ICR mice (8 weeks old, 29–32 g, Beijing Vital River Laboratory Animal Technology Co., Ltd.) were housed under a 12/12 light/dark cycle at constant room temperature (23 ± 1℃) with free access to food and water before initiation of the experiment. All procedures for animal care and use were carried out by following the guidelines for the care and use of laboratory animals. All efforts were made to reduce the number of animals used and bodily suffering during the experiment. This study was approved by the Medical Ethics Committee of Yantai Yuhuangding Hospital (No. 2020–155).
5
Mice Housing Conditions for Research
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Male ICR mice at 8–10 weeks of age were purchased from Vital River Laboratory Animal Technology Co., Ltd., Beijing. All mice were housed in a room with a constant temperature of 23°C–25°C and with a 12-h light/dark cycle. Mice were allowed free access to food and water. This study was approved by the Research Ethics Committee of the Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China (Approval Number: 2019B035). All animal studies were carried out according to the recommendations of the ethical guidelines and regulations for the care and use of laboratory animals.
6
Mice Housing and Berberine Hydrochloride Procurement
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Male ICR mice weighing 18–22 g were purchased from Vital River Laboratories (Beijing, China). The animals were housed in temperature- and humidity-controlled rooms, kept on a 12 h light/dark cycle and provided with unrestricted amounts of rodent chow and drinkable water. The laboratory animal facility was accredited by the AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care International), and the IACUC (Institutional Animal Care and Use Committee) of Tsinghua University approved all animal protocols used in this study (Approval ID: 13-DLJ1).
Berberine hydrochloride was purchased from the Beijing Shuanghe Pharmacy Co. Ltd. (Batch No. 110905) (Beijing, China). Berberine hydrochloride standards were purchased from the National Institutes for Food and Drug Control (Beijing, China) (Batch No. 110713–200609). Isolated alcohol (analytical grade) was purchased from Beijing Chemical Plant (Beijing, China).
Berberine hydrochloride was purchased from the Beijing Shuanghe Pharmacy Co. Ltd. (Batch No. 110905) (Beijing, China). Berberine hydrochloride standards were purchased from the National Institutes for Food and Drug Control (Beijing, China) (Batch No. 110713–200609). Isolated alcohol (analytical grade) was purchased from Beijing Chemical Plant (Beijing, China).
7
Murine Models of Type 2 Diabetes
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Male ICR mice weighing 20–22 g were purchased from Vital River Laboratory Animal Technology Co. Ltd. (Beijing, China) and housed five per cage with access to standard chow (Research Diets, Inc., Beijing, China) and water at constant room temperature (22 ± 3°C) in a 12 h light/dark cycle.
Female db/db (BKS.Cg-m+/+Leprdb/J) mice aged 6-7 weeks were purchased from Changzhou Cavens Laboratory Animal Co. Ltd. (Changzhou, China). db/db mice were housed four or three per cage at constant room temperature (22 ± 3°C) in a 12 h light/dark cycle and fed ad libitum with a special diet (protein content is higher; other ingredients are the same as standard diet) supplied by Changzhou Cavens Laboratory Animal Co. Ltd. (Changzhou, China). Age-matched heterozygotes db/m mice were used as control animals. db/m mice were housed five per cage under the same conditions and fed ad libitum with standard diet (Research Diets, Inc., Beijing, China).
All animals were handled in accordance with the standards for laboratory animals established by China (GB14925-2001), and all efforts were made to minimize suffering.
Female db/db (BKS.Cg-m+/+Leprdb/J) mice aged 6-7 weeks were purchased from Changzhou Cavens Laboratory Animal Co. Ltd. (Changzhou, China). db/db mice were housed four or three per cage at constant room temperature (22 ± 3°C) in a 12 h light/dark cycle and fed ad libitum with a special diet (protein content is higher; other ingredients are the same as standard diet) supplied by Changzhou Cavens Laboratory Animal Co. Ltd. (Changzhou, China). Age-matched heterozygotes db/m mice were used as control animals. db/m mice were housed five per cage under the same conditions and fed ad libitum with standard diet (Research Diets, Inc., Beijing, China).
All animals were handled in accordance with the standards for laboratory animals established by China (GB14925-2001), and all efforts were made to minimize suffering.
8
ICR Mice Housing Conditions
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Male ICR mice were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China), and housed in a room with a constant temperature of 22–23 °C and a 12-h light-dark cycle. The mice had free access to food and water. The study was approved by the Animal Care and Ethics Committee of Qingdao Agricultural University and was conducted in accordance with the National Institutes of Health (NIH) guidelines for the care and use of laboratory animals (NIH Publications no. 8023).
9
Mice Feeding Study on Red Sufu
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Male ICR mice (weighing from 18 to 20 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). They were housed in a SPF level room with a 12/12 h light-dark alternation cycle at constant room temperature of 23 ± 1°C and moderate humidity (55 ± 5%). Mice were allowed to adopt the surrounding environment for one week before experimental treatments were conducted. After adaptation, 50 mice were randomly divided into 5 groups each containing 10 mice. Mice were fed ad libitum continuously for 15 days on a commercial rodent diet and gavaged with distilled water (Group W), commercial Wang Zhihe red sufu (Group C), 0.5% IF sufu (Group L), 1% IF sufu (Group M) and 2% IF sufu (Group H). The administration dosage was 9.2 g/kg body mass per day.
10
CdTe Quantum Dot Exposure in Mice
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Male ICR mice, which were 6-8 weeks of age, provided from Beijing (Vital River Laboratory Animal Technology Co., Ltd., Beijing, China). All procedures and experimentations were approved by the Ethics Committee of Capital Medical University (ethical review number: AEEI-2016-184). These mice were raised in a standardized animal house at China Capital Medical University with a 12-hour day/dark revolution, in which operation was ventilated, sterile and temperature-controlled. Regular observation was conducted for each mouse containing fur, urine, mental status, behaviour, food intake and faces.
After 7 days of acclimatization, mice were administered the CdTe aqQD solutions by tail intravenous injection of 2 µmol/kg (based on the molar mass of Cd) between 33.1 and 35.8 g in weight, the control was injected physiological saline with an equivalent volume. Five mice from each exposed group were anaesthetized with isoflurane at the predetermined time points (1, 7 days), which were killed using cervical dislocation, their kidneys (1 day) were collected for gene microarray analysis, and the kidney and liver tissues (1, 7 days) were analysed with real-time qPCR. Sets of control mice were also killed at the predetermined times (1, 7 days) in strict accordance with the programs used for treated mice.
After 7 days of acclimatization, mice were administered the CdTe aqQD solutions by tail intravenous injection of 2 µmol/kg (based on the molar mass of Cd) between 33.1 and 35.8 g in weight, the control was injected physiological saline with an equivalent volume. Five mice from each exposed group were anaesthetized with isoflurane at the predetermined time points (1, 7 days), which were killed using cervical dislocation, their kidneys (1 day) were collected for gene microarray analysis, and the kidney and liver tissues (1, 7 days) were analysed with real-time qPCR. Sets of control mice were also killed at the predetermined times (1, 7 days) in strict accordance with the programs used for treated mice.
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