Take 20–30 μL of peripheral blood through the tail vein of the mouse and add it to the anticoagulation tube. Take the bone marrow cells from the femur and tibia of the sacrificed mice. The red blood cells were lysed, and the bone marrow cells were filtered using a 100-mm cell strainer. Monoclonal antibodies to Mac-1 (M1/70, Biolegend), Gr-1 (RB6-8C5, Biolegend), c-Kit (2B8, Biolegend), Lin mix (Gr1, CD4, CD3, CD8a, Ter119, B220, IgM) (Biolegend), CD34 (MEC14.7, Biolegend), Sca1 (D7, Biolegend), FcgRII/III (93, Biolegend), IL-7Ra (A7R34, Biolegend) (all used 50 ng per million cells) were used where indicated. After incubation with antibodies, the samples were analyzed using the Attune NxT flow cytometer (Thermo), and the results were analyzed using FlowJo software. Here, 7-aminoactinomycin D (7-AAD) (A1310, Life Technologies) was used to exclude dead cells.
Mac 1 m1 70
Manufactured by BioLegend
Sourced in United States
The Mac-1 (M1/70) is a murine monoclonal antibody that recognizes the CD11b antigen, also known as the alpha M chain of the Mac-1 integrin (CD11b/CD18). CD11b is expressed on the surface of various immune cells, including monocytes, granulocytes, and natural killer cells. This antibody can be used for the identification and characterization of CD11b-positive cells in flow cytometry and other immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Gr 1 rb6 8c5, by BioLegend (3 mentions)
C kit 2b8, by BioLegend (3 mentions)
Sca1 d7, by BioLegend (3 mentions)
Cd34 mec14, by BioLegend (2 mentions)
Il 7ra a7r34, by BioLegend (2 mentions)
7 aminoactinomycin d 7 aad, by Thermo Fisher Scientific (2 mentions)
Attune nxt flow cytometer, by Thermo Fisher Scientific (2 mentions)
B220 ra3 6b2, by BioLegend (2 mentions)
Cd150 tc15 12f12.2, by BioLegend (2 mentions)
Gr 1 rb6 8c5, by BD (1 mentions)
Flt 3 a2f10.1, by BD (1 mentions)
Cd45.1 a20, by BD (1 mentions)
Cd19 6d5, by BioLegend (1 mentions)
Cd45.2 104, by BD (1 mentions)
Cd31 390, by BioLegend (1 mentions)
Cd140a apa5, by BD (1 mentions)
Anti c kit 2b8, by BD (1 mentions)
Cd8a 53 6, by BioLegend (1 mentions)
Igm mhm 88, by BioLegend (1 mentions)
Cd4 rm4 5, by BioLegend (1 mentions)
4 protocols using mac 1 m1 70
1
Immunophenotyping Murine Hematopoietic Cells
2
Multiparameter Flow Cytometry Immunophenotyping
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The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences), -Sca-1 (E13-161.7/D7, BioLegend), -CD140a (APA5, BD Biosciences), -CD51 (RMV-7, BIO-RAD), -CD4 (RM4-5, BioLegend), -CD8a (53-6.7, BioLegend), -B220 (RA3-6B2, BioLegend/WAKO), -CD31 (390, BioLegend), -TER-119 (TER-119, BioLegend/BD Biosciences), -Gr-1 (RB6-8C5, BD Biosciences), -Mac-1 (M1/70, BioLegend), -CD48 (HM48-1, BD Biosciences), -CD150 (TC15-12F12.2, BioLegend), -CD19 (6D5, BioLegend), -IgM (MHM-88, BioLegend), -CD43 (S11, BioLegend), -CD45.1 (A20, BD Biosciences), -CD45.2 (104, BD Biosciences), -CD45 (30-F11, BioLegend), -Flt3 (A2F10.1, BD Biosciences), -IL7Rɑ (A7R34, TONBO biosciences), -FcγR (2.4G2, TONBO biosciences), -53BP1 (Novus Biologicals). A biotinylated CD34 (RAM34, Thermo Fisher Scientific) and a fluorochrome labelled streptavidin (BD Biosciences/BioLegend) were used.
3
Isolation and Characterization of Murine Hematopoietic Cells
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Take 20–30 μl of peripheral blood through the tail vein of the mouse and add to the anticoagulation tube. Take the bone marrow cells from the femur and tibia of the sacrificed mice. The red blood cells were lysed, and the bone marrow cells were filtered using a 100-μm cell strainer. Monoclonal antibodies to Mac-1 (M1/70, Biolegend), Gr-1 (RB6-8C5, Biolegend), c-Kit (2B8, Biolegend), Lin mix (Gr1, CD4, CD3, CD8a, Ter119, B220, IgM) (Biolegend), CD34 (MEC14.7, Biolegend), Sca1 (D7, Biolegend), FcγRII/III (93, Biolegend), IL-7Ra (A7R34, Biolegend) (all used as 50 ng per million cells) were used where indicated. After incubation with antibodies, the samples were analyzed using the Attune NxT flow cytometer (Thermo), and the results were analyzed using FlowJo software. Here, 7-aminoactinomycin D (7-AAD) (A1310, Life Technologies) was used to exclude dead cells.
4
Monoclonal Antibodies for Cell Identification
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The monoclonal antibodies used in this study targeted the following proteins: CRLR (H-42; Santa Cruz, Dallas, TX, USA), RAMP-1 (EPR10867; abcam, Cambridge, UK), Gr-1 (RB6-8C5; BioLegend, San Diego, CA, USA), Mac-1 (M1/70; BioLegend), B220 (RA3-6B2; BioLegend), CD3e (145-2C11; Biolegend), CD2 (RM2-5; TONBO, Japan), CD8a (53–6.72; TONBO), TER-119 (TER-119; TONBO), CD127 (A7R34; TONBO), c-Kit (2B8; BioLegend), Sca-1 (D7; BioLegend), CD34 (RAM34; eBioscience, Santa Clara, CA, USA), CD16/32 (93; eBioscience), CD48 (HM48-1; BioLegend), CD150 (TC15–12F12.2; BioLegend), CD45.2 (104; eBioscience) and CD45.1 (A20; eBioscience). A mixture of mAbs against CD2, CD3e, CD8a, B220, TER-119, CD127, Mac-1 and Gr-1 served as lineage markers (Lineage). Mouse anti-BrdU-Alexa Fluor 647 antibody (3D4; BD Biosciences) was used to detect intracellular BrdU.
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