To silence the expression of TGF-β1, short hairpin RNA against TGF-β1 (sh-TGF-β1) and the negative control shRNA (sh-NC) were purchased from RiboBio, Guangzhou, China. Cells were transfected with sh-TGF-β1 (5 nM) or sh-NC (5 nM) by using Lipofectamine 3000 (Invitrogen, Waltham, MA, USA). The transfection efficacy was evaluated by quantitative real-time PCR (qPCR) assay after 48 h of transfection.
Sh tgf β1
Manufactured by RiboBio
Sourced in China
Sh-TGF-β1 is a laboratory product designed for scientific research purposes. It functions as a small hairpin RNA (sh-RNA) that targets the transforming growth factor beta 1 (TGF-β1) gene. This product is intended for use in cellular and molecular biology experiments to study the role of TGF-β1 in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Sh nc, by RiboBio (2 mentions)
Sh nc, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Lipo3000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Oe nc, by RiboBio (1 mentions)
Mir 124 mimic, by RiboBio (1 mentions)
Mir 124 inhibitor, by RiboBio (1 mentions)
2 protocols using sh tgf β1
1
Silencing TGF-β1 Expression using shRNA
2
miR-124 Regulates TGFβ1 and DNMT1 in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cells in the logarithmic phase of growth were detached with trypsin and seeded in a six-well plate, at a density of 1 × 105 cells per well. After 24 h of conventional culture, the cells were transfected with 250 μl of Lipo3000 transfection reagent (Invitrogen) at room temperature for 0 to 15 min, and the solution was changed after 16 h. The RNA content was extracted after 48 h, followed by protein extraction after 72 h. The details of experimental grouping were described in the Results. mimic-negative control (NC), miR-124 mimic, inhibitor NC, miR-124 inhibitor, sh-NC, sh-TGFβ1, oe-NC, oe-TGFβ1, sh-DNMT1, and oe-DNMT1 were all purchased from Ribobio (Guangzhou, China).
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