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Anhydrous ethanol

Manufactured by Beijing Chemical Works
Sourced in China

Anhydrous ethanol is a pure, water-free form of ethanol. It is a colorless, flammable liquid with a characteristic odor. Anhydrous ethanol is a versatile chemical compound used for various laboratory and industrial applications.

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32 protocols using anhydrous ethanol

1

Synthesis of Silver Nanoparticles

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Ammonium hydroxide (28.0–30.0 wt%, NH3), tetraethylorthosilicate (TEOS, 98.0%), and 3-mercaptopropyl trimethoxysilane (MPTS, 95.0%) were purchased from Alfa Aesar (Shanghai, China). Ethylene glycol (EG, 99.0%) was from Aladdin (Shanghai, China). Polyvinylpyrrolidone (PVP, Mw = 40,000) and octylamine (OA, 99.5%) were from Sigma-Aldrich (St. Louis, MO, USA). Anhydrous ethanol (99.5%) was from Beijing Chemical Works (Beijing, China) and silver nitrate (AgNO3, 99.8%) from Guanghua Sci-Tech Co., Ltd. (Guangzhou, China). Thiram was purchased from J&K scientific (Beijing, China). All chemicals and reagents were used as received without further purification. Deionized (DI) water was purified by a Milli-Q water filtration system (Millipore, Milford, MA, USA). Apple juice (Huiyuan) was purchased from a local supermarket in Beijing.
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2

Carboxyl Nitrile Rubber Functionalization

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Carboxyl nitrile rubber with acrylonitrile content of 28%, carboxyl content of 8.5%, and the molecular of 1.5 × 104 was obtained from Zeon Corporation; anhydrous ethanol and chlorobenzene were purchased from Beijing Chemical Plant (Beijing, China); Octanol (C8H17OH), Dodecanol (C12H25OH), Cetyl alcohol (C16H33OH), Stearyl alcohol (C18H37OH) and Methoxypolyethylene glycols (Mn = 350, 750, 1000) were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China) Triethylamine (Et3N) and Thionyl Chloride (SOCl2) were purchased from Shanghai Sigma-Aldrich Co., Ltd. (Shanghai, China) Deuterated chloroform was obtained from National Chemical Reagent Co (Beijing, China).
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3

Immunohistochemical Analysis of Cellular Markers

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The following reagents were purchased from Beijing Bioss Biotechnology: c-fos rabbit anti-mouse polyclonal antibody, ZO-1 rabbit anti-mouse polyclonal antibody, Occludin rabbit anti-mouse polyclonal antibody, myosin light chain kinase (MLCK) rabbit anti-mouse polyclonal antibody, P-glycoprotein (P-gp) rabbit anti-mouse polyclonal antibody, multidrug resistance associated protein 1 (MRP1) rabbit anti-mouse polyclonal antibody, multidrug resistance associated protein 2 (MRP2) rabbit anti-mouse polyclonal antibody, goat anti-rabbit immunohistochemistry kit, and hematoxylin. Other reagents used were as follows: glacial acetic acid, analytical grade (Beijing Chemical Works, Batch No. 20100603); osmic acid, acetone, embedding media, uranyl acetate stain, and lead citrate stain (supplied by the Experimental Research Center); potassium permanganate, analytical grade (Beijing Chemical Works, Batch No. 820308); toluidine blue, analytical grade (Sinopharm Chemical Reagents, Batch No. WC20050120); anhydrous ethanol, analytical grade (Beijing Chemical Works, Batch No. 20110); xylene, analytical grade (Sinopharm Chemical Reagents, Batch No. 20106); neutral gum (Sinopharm Chemical Reagents, Batch No. 20120320); hematoxylin (Beyotime Biotechnology, Batch No. C0105); ethylenediamine tetraacetic acid (EDTA), analytical grade (Beijing Chemical Works, Batch No. 840529).
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4

Ginsenoside Re Extraction Protocol

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GFs of various ages were collected in June 2019 from Fusong (Jilin) and authenticated by Professor Changbao Chen (Changchun University of Chinese Medicine). After steaming at 40°C for 48 h, then crushed through 20-mesh sieves.
Ginsenoside Re (purity >98%) was obtained from the Department of Organic Chemistry, Jilin University; bovine serum albumin (BSA) was purchased from the Chinese Academy of Metrology; the glucose standard was purchased from Beijing Putian Tongchuang Biotechnology Co., Ltd. (Beijing, China); n-alkanes (C8-C30) was purchased from Alfa Aesar (Tewksbury, MA, USA); methanol, anhydrous ethanol, concentrated sulfuric acid, and glacial acetic acid (all analytically pure) were purchased from Beijing Chemical Plant (Beijing, China); polydimethylsiloxane-divinylbenzene solid-phase microextraction head (PDMS-DVB 65 μm) and 12 mL headspace vials were purchased from Supelco (Bellefonte, PA, USA).
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5

Multimodal Analysis of Fingerprint Evidence

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The equipment used was a Q600 SDT thermogravimetric-differential scanning calorimetry integrated thermal analyser (TA Company, New Castle, DE, USA); SolidSpec-3700 ultraviolet-visible near-infrared spectrophotometer (Shimadzu Corporation, Kyoto, Japan); Fluorolog®-Tau-3 fluorescence spectrometer (Jobin Yvon Company, Longjumeau, France); MVC3000 handprint fuming cabinet, DCS-4 handprint photographing and inspection system (Foster + Freeman Company, Evesham, UK).
The chemicals used were PolyCyano UV (powder, 95%); Cyanobloom (colloid, 95%, Foster + Freeman Company, Shanghai, China); Rhodamine 6 G (98%); BBD (98.53%, MCE Company); anhydrous ethanol; acetone (analytical grade, Beijing Chemical Plant, Beijing, China); and all water was deionised water.
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6

Lipid Profiling by GC-MS

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Assay kits for total cholesterol, triglycerides, high-density lipoprotein, blood glucose, and alanine aminotransferase (ALT) were obtained from the Zhongsheng Co. Cholesterol, 5A-cholestane, desmosterol, lathosterol, squalene, campesterol, stigmasterol, and sitosterol were obtained from Sigma-Aldrich. KOH (analytical pure), anhydrous ethanol (chromatographic pure), and n-hexane (chromatographic pure) were obtained from Beijing Chemical Reagent Company. The HMDS: TMCS: pyridine ratio was 3: 1: 9 and was obtained from Supelco Co. The experimental water used was ultrapure water obtained from Millipore.
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7

Zinc Chloride-Mediated Polymer Synthesis

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Zinc chloride was obtained from Tianjin Guangfu Technology Development Co., Ltd. Anhydrous ethanol was purchased from Beijing Chemical Works. p-Phenylenediamine (PPD) was acquired from National Drug Reagent Co., Ltd. All chemicals were used as received. Polyvinylpyrrolidone K54–K65 (PVP) was obtained from Saen Chemical Technology (Shanghai) Co., Ltd. Its average Mw is around 360 000. All the materials employed in the experiment are of AR grade except PPD, which is of CP. The microporous membrane (nylon 66, 0.22 μm) were purchased from Tianjin Jinteng Experimental Equipment Co. Ltd.
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8

Graphene Oxide-Humic Acid Composite Synthesis

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Ferric trichloride (FeCl3, AR), sodium hydroxide (NaOH), hydrazine (N2H4·H2O, 98%), and (3-Aminopropyl)triethoxysilane (APTES) were purchased from Aladdin Industrial Co., Ltd. (Shanghai, China). GO (powder in a single layer) was purchased from Jiangsu Xianfeng Nanomaterials Technology Co., Ltd. (Nanjing, China). Cupric chloride (CuCl2, AR) was purchased from Sinopharm Chemical Reagent Beijing Co., Ltd. (Beijing, China). Humic acid (HA, CP) was purchased from Guangfu Chemical Reagent Company (Tianjin, China). Anhydrous ethanol and hydrochloric acid (HCl, 37%) were purchased from Beijing Chemical Reagent Company (Beijing, China). Deionized water was made in the laboratory and used for experiments.
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9

Surface Modification of Carbon Fibre Tows

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CF tow (T800-6K) and anodised carbon fibre (ECF) tow (T800-6K) were all made by Key Laboratory of Carbon Fibre and Functional Polymer Ministry of Education, Beijing University of Chemical Technology, without sizing agent on the surface. Glycidyl methacrylate (GMA) (99%) was purchased from Adamas Reagent Co., Ltd. Aluminium trichloride (AlCl3) was purchased from Tianjin Fuchen Chemical Reagent Factory. Anhydrous ethanol (>99%) was purchased from Beijing Chemical Plant. Tetrahydrofuran (THF) (>99%) and diiodomethane (>99%) was purchased from Beijing Tongguang Fine Chemical Company.
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10

Comprehensive Acetylcholinesterase Inhibition Assay

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β-glucosidase (food grade), arctiin standards (Lot: 120605), and arctigenin standards (Lot: 120509) were purchased from Chengdu Biological Technology Co., LTD. (Chengdu, China). HPLC grade methanol was purchased from Fisher Scientific (Shanghai, China). Analytical grade methanol and anhydrous ethanol were purchased from Beijing Chemical Works (Beijing, China). Purified water was purchased from Hangzhou Wahaha Group Co., LTD (Hangzhou, China). SPECTRA MAX190 (United States) acetylthiocholine iodide (ATCI, corresponding to 1 mM diluted in phosphate-buffered saline [PBS]), AChE (0.28 U/mL) (C3389 from electric Electrophorus electricus [electric eel], Type VI-S lyophilized powder, 200–1000 units/mg protein, store: −20), 5,5'-dithiobis-(2-nitrobenzic acid) (DTNB), Huperzine A (sigma), PBS buffer (PH = 8), and dimethyl sulfoxide (DMSO) were purchased from Sigma (USA). Ninety-six-well culture plates were purchased from Falcon, VC,1 mM NaNO2, and 0.4% sulfanilic acid.
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