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Integrin β 5

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Integrin β-5 is a cell surface glycoprotein that functions as a receptor for a variety of extracellular matrix proteins. It plays a role in cell-cell and cell-matrix interactions, and is involved in various cellular processes such as cell adhesion, migration, and signaling.

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Lab products found in correlation

Su6656, by Merck Group (1 mentions) Integrin β1, by Cell Signaling Technology (1 mentions) Integrin α5, by Cell Signaling Technology (1 mentions) Integrin αv, by Cell Signaling Technology (1 mentions) Perk t202 y204, by Cell Signaling Technology (1 mentions) P mapkapk2, by Cell Signaling Technology (1 mentions) P paxillin, by Cell Signaling Technology (1 mentions) P sapk jnk, by Cell Signaling Technology (1 mentions) P p38 mapk, by Cell Signaling Technology (1 mentions) Smad2 3, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) Sapk jnk, by Cell Signaling Technology (1 mentions) P src, by Cell Signaling Technology (1 mentions) Integrin β4, by Santa Cruz Biotechnology (1 mentions) Mapkapk2, by Cell Signaling Technology (1 mentions) P38 mapk, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Fibronectin, by BD (1 mentions) Axiovision software, by Zeiss (1 mentions)

2 protocols using integrin β 5

1

Integrin-Mediated Signaling Pathways

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Reagents. DMSO (Sigma, D8401), SB203580 (Calbiocam, 559389), SU6656 (Sigma, S9692), and RGD peptides (Sigma, A8052).
Antibodies. Fibronectin (BD Biosciences, 610077), α-tubulin(Sigma, T5168), β-actin (Sigma, A5441), integrin β-1 (Cell Signaling Technology, 4706), integrin β-3(D7X3P) (Cell Signaling Technology, 13166), integrin β-4 (Santa Cruz Biotechnology, sc-514426), integrin β-5 (Santa Cruz Biotechnology, sc-5402), integrin α-5 (Cell Signaling Technology, 4705), integrin α-v (Cell Signaling Technology, 4711) ERK1 (Santa Cruz Biotechnology, sc-94), p-ERK(T202/Y204) (Cell Signaling Technology, 9101), AKT (Cell Signaling Technology, 9272), p-AKT(S473) (Cell Signaling Technology, 9271), SAPK/JNK (Cell Signaling Technology, 9258), p-SAPK/JNK(T183/Y185) (Cell Signaling Technology, 9251), Smad2/3 (Cell Signaling Technology, 3102), Src (Cell Signaling Technology, 2110), p-Src(Y416) (Cell Signaling Technology, 2101), p38MAPK (Cell Signaling Technology, 9211), p-p38MAPK (Cell Signaling Technology, 9212), MAPKAPK2 (Cell Signaling Technology, 3042), p-MAPKAPK2(Thr334) (Cell Signaling Technology, 3007), and), p-paxillin (Y118) (Cell Signaling Technology, 2541).
Park H.J, & Helfman D.M. (2019). Up-regulated fibronectin in 3D culture facilitates spreading of triple negative breast cancer cells on 2D through integrin β-5 and Src. Scientific Reports, 9, 19950.
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2

Integrin β5 Immunohistochemistry in Rat and Human Lung

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Paraffin‐embedded and formalin‐fixed rat lung tissue was sectioned at a thickness of 3 μm. Serial sections of human lung tissue were obtained from the Department of Pathology, University of Colorado Denver and the PHBI. We previously published the details of immunohistochemistry (IHC) using heat‐induced antigen retrieval in citrate buffer at pH 6.0.4, 42, 44 The following primary antibody was used: Integrin β5 (sc‐390186, Santa Cruz Biotechnology). A secondary antibody conjugated to biotin, followed by horseradish peroxidase conjugated streptavidin and 3,3'‐diaminobenzidine staining reaction, were used for IHC detection. Controls with unspecific Immunoglobin G were run in parallel with each staining batch. Images were taken with an AXIO imager.A1 microscope, Axiocam HRc camera and Axiovision software (all Zeiss). Slides for anti‐integrin‐ανβ5 antibody treated rats were sectioned, stained and scanned by Histowiz.
Blanchard N., Link P.A., Farkas D., Harmon B., Hudson J., Bogamuwa S., Piper B., Authelet K., Cool C.D., Heise R.L., Freishtat R, & Farkas L. (2022). Dichotomous role of integrin‐β5 in lung endothelial cells. Pulmonary Circulation, 12(4), e12156.
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