Ethylene diamine tetraacetic acid (edta)

Manufactured by HiMedia

Sourced in India

EDTA is a chelating agent used in laboratory settings. It is commonly used for the removal and sequestration of metal ions from solutions. EDTA forms stable complexes with a variety of metal ions, making it a valuable tool for various analytical and experimental procedures.

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Lab products found in correlation

24 protocols using ethylene diamine tetraacetic acid (edta)

Cell Line Authentication by STR Profiling

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Unless otherwise mentioned all reagents were of the highest purity available and obtained from Sigma Aldrich (now Merck, St. Louis, MO, USA); Agarose, ethidium bromide, catalase, ethanol, DMSO, NaCl, EDTA, Tris-Cl were obtained from HiMedia (Mumbai, India); DMEDA from Tokyo Chemical Industry (Tokyo, Japan); acetonitrile from JT Baker (Center Valley, PA, USA); glutathione from Sisco Research Lab. Pvt Ltd. (Mumbai, India); 2′-deoxyadenosine from Alfa Aesar (Haverhill, MA, USA); ethylamine and propylamine from Spectrochem Pvt Ltd. (Mumbai, India).
Cell lines authentication by STR profiling was outsourced to Lifecode Technologies (New Delhi, India).

Siddiqui S., Ahmed N., Goswami M., Chakrabarty A, & Chowdhury G. (2021). DNA damage by Withanone as a potential cause of liver toxicity observed for herbal products of Withania somnifera (Ashwagandha). Current Research in Toxicology, 2, 72-81.

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Cytotoxicity Evaluation of Compounds

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Growth medium (MEM/RPMI), fetal calf serum, trypsin, penicillin, streptomycin, DMSO, proteinase K, RIPA Buffer, bisacrylamide, SDS, MTT dye, acrylamide, ammonium persulfate (APS), N, N, N’, N’ tetramethylethylenediamine (TEMED), 2-mercaptoethanol, DAPI, Tris base. All the above mentioned chemicals were obtained from Sigma. Chemiluminescent western blotting kit (Millipore), Quanti Pro BCA assay kit, 96 and 6 well plate (Iwaki), triton X (Hi-Media), EDTA (Hi-Media), ELISA plate reader (Bio-Rad). NFκB (p65), caspase-3 antibodies were purchased from Millipore Pvt Ltd.

Qazi A.K., Hussain A., Aga M.A., Ali S., Taneja S.C., Sharma P.R., Saxena A.K., Mondhe D.M, & Hamid A. (2014). Cell specific apoptosis by RLX is mediated by NFκB in human colon carcinoma HCT-116 cells. BMC Cell Biology, 15, 36.

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Docetaxel-loaded PLGA Nanoparticles

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TherDose Pharma Pvt Ltd. generously provided docetaxel (DTX) and poly(d,l-lactic-co-glycolic acid) (PLGA) with a free carboxyl end group (uncapped) and an L/G molar ratio of 50:50, (Hyderabad, Andhra Pradesh, India) and Evonik (Mumbai, Maharashtra, India), respectively. ADN, Tween 80, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), Formaldehyde, Hoechst blue 33342 Rhodamine 6G, chloroform, methanol, acetone, dichloromethane, phosphotungstic acid, mannitol, dimethyl sulfoxide (DMSO), and acetonitrile were of HPLC grade (Merck, Mumbai, Maharashtra, India). The A549 cell line was obtained from the National Centre for Cell Science (NCCS, Pune, Maharashtra, India). Dulbecco’s modified Eagle’s Medium (DMEM), fetal bovine serum MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide), trypsin, EDTA, 2-(N-morpholino) ethanesulfonic acid (MES), Triton, and 96-well flat bottom tissue culture plates were purchased from Himedia (Mumbai, Maharashtra, India). Dialysis tubes were purchased from Spectrum (Float-A-Lyzer (G2, Spectrum, Repligen, MA, USA).

Aldawsari H.M., Singh S., Alhakamy N.A., Bakhaidar R.B., Halwani A.A., Sreeharsha N, & Badr-Eldin S.M. (2022). Adenosine Conjugated Docetaxel Nanoparticles—Proof of Concept Studies for Non-Small Cell Lung Cancer. Pharmaceuticals, 15(5), 544.

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Formulation and Evaluation of Cytotoxicity

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Egg albumin powder was purchased from
Otto Mumbai, India. Tween
80, non-ionic surfactant (polyoxyethylenesorbitan mono-oleate), and
ethanol (absolute) were obtained from SD Fine Chemicals, Mumbai, India.
Tramadol hydrochloride was obtained as a gift sample from a pharmaceutical
company in India. 3-(4,5-Dimethylthiazol-2-yl)-5-diphenyltetrazolium
bromide (MTT), fetal bovine serum (FBS), phosphate-buffered saline
(PBS), Dulbecco’s modified Eagle’s medium (DMEM), zein,
and trypsin were purchased from Sigma-Aldrich Co., St. Louis, USA.
EDTA (ethylenediaminetetraacetic acid), glucose, and antibiotics were
procured from Hi-Media Laboratories Ltd., Mumbai, India. Dimethyl
sulfoxide (DMSO), acetone, and propanol were bought from Merck Ltd.,
Mumbai, India. The 3T3L1 (mouse embryonic fibroblast - adipose like
cell line) cell line was procured from the National Centre for Cell
Sciences (NCCS), Pune, India.

Agrawal S., Patel P.R, & Gundloori R.V. (2019). Proteins as Nanocarriers To Regulate Parenteral Delivery of Tramadol. ACS Omega, 4(4), 6301-6310.

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Enzymatic Assay for Antioxidant Activity

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6-Methoxy-2-naphthaldehyde, 6-methoxy-2-naphthoic acid, bovine serum albumin (BSA), bicinchoninic acid solution (BCA) and NAD⁺ were procured from Sigma Chemicals Co., USA. Dithiothreitol (DTT) was obtained from Sisco Research Laboratories, India. Thiobarbituric acid, Di-sodium hydrogen phosphate, sodium phosphate monobasic anhydrous, EDTA and Tris were the products of Himedia chemicals (India). Tricholoroacetic acid was from Qualigens, India. NaOH and HCl (35%) were obtained from Merck Specialities Pvt. Ltd. The other reagents and chemicals used were all of analytical grade.

Younus H., Ahmad S, & Alam M.F. (2020). Correlation between the Activity of Aldehyde Dehydrogenase and Oxidative Stress Markers in the Saliva of Diabetic Patients. Protein and Peptide Letters, 27(1), 41-47.

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Bacterial Protein Expression and Purification

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The principal components of bacterial culture: LB broth, tryptone, and yeast extract; antibiotics: kanamycin; protein expression and extraction reagents: IPTG, Tris–HCL and base, glycine, lysozyme, PMSF, SDS, acrylamide/bis-acrylamide, ammonium persulfate, tetramethylethylenediamine (TEMED), DTT, and EDTA, and Coomassie G-250 were purchased from Himedia and VWR life science. Inclusion body solubilizing and protein refolding reagents: guanidine hydrochloride, urea, arginine hydrochloride, and isopropanol were from Sigma-Aldrich. PCR cloning kit: pJET1.2; Q5 high fidelity DNA polymerase and restriction enzymes, T4-DNA ligase, and rapid protein assay BCA kit were from New England Biolabs (NEB) and Thermo Scientific. Protein purification was performed by AKTA FPLC systems using superose 12 10/300 size-exclusion column which were purchased from GE Healthcare. Surface Plasmon Resonance instrument (Autolab ESPRIT) and BLI systems (Octet RED96) were used to measure the binding affinity.

Sarker A., Rathore A.S., Khalid M.F, & Gupta R.D. (2022). Structure-guided affinity maturation of a single-chain variable fragment antibody against the Fu-bc epitope of the dengue virus envelope protein. The Journal of Biological Chemistry, 298(4), 101772.

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Pantoprazole Characterization and Chloride Salt Interactions

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The medicine namely Pantoprazole (sensor1) which has an expiry date of 04/2019 was used for the current study and to conduct experiments. Pantoprazole has a molecular weight of 383.4 g/mol and molecular formula C16H15F2N3O4S. It melts at about 150 0 C and has a boiling point of 586.9 0 C. Its IUPAC name is 6-(difluoromethoxy)-2-[(3,4-dimethoxypyridin-2yl)methylsulfinyl]-1H-benzimidazole. It belongs to benzimidazole class. It is a member of organofluorine, sulphoxide, aromatic ether, and pyridines. Pantoprazole is generally used in the treatment of stomach ulcers. [11] The compound was taken from a local shop City Drug House, Dharwad, Karnataka, India. The tablet was powdered finely using a mortar for conducting experiments.
The salts NaCl,CuCl2.2H2O,CoCl2.6H2O,HgCl2,KCl,ZnCl2,FeCl3,PbCl2,MnCl2.4H2O,CaCl2,MgCl2, BaCl2, LiCl, AgCl, CdCl2 were of analytical grade and purchased from Sisco Research Laboratories Pvt. Ltd. India. EDTA was bought from HiMedia Laboratories Pvt. Ltd. India. All purchased chemicals were ultrapure and used without any modifications. Highly pure distilled water was taken to perform all the experiments. The same room temperature that is 25 0 C was maintained throughout the investigation.

Nadgir A, & Sidarai A.H. (2021). Photophysical Investigation of a Benzimidazole Derivative and Its Applications in Selective Detection of Fe³⁺, Thermosensing and Logic Gates. Journal of fluorescence, 31(5).

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Quantification of Oxidative Stress

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Sodium arsenite solution (CAS No. 7784-46-5) was obtained from Sigma-Aldrich Laborchemikalien, Germany. NaCl, eosin (2 % w/v), Giemsa, EDTA, TCA, DTNB, 2-thiobarbituric acid and methanol were procured from HIMEDIA Laboratories, India. Tris HCl and pyragallol were purchased from Sisco Research Laboratories Pvt. Ltd, India. Stains and reagent solutions were prepared in double distilled water.

Das S., Upadhaya P, & Giri S. (2016). Arsenic and smokeless tobacco induce genotoxicity, sperm abnormality as well as oxidative stress in mice in vivo. Genes and Environment, 38, 4.

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Comprehensive Reagents and Cell Lines

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Unless otherwise mentioned all chemicals used are of the highest quality available and purchased from Sigma Aldrich (St. Louis, MO, USA). agarose, agar, ethidium bromide, catalase, m ethanol, ethanol, isopropanol, DMSO, NaCl, EDTA, Tris-HCl, Triton-X 100, NaOH, paraformaldehyde, bovien serum albumin (BSA), biotin, histidine, ampicillin, oxoid nutrient broth No. 2, and protease inhibitor cocktail were obtained from HiMedia (Mumbai, India), sodium fluoride and orthovandate from MP Biomedicals, LLC (Solon Ohio, USA), CellROX Deep Red and Nuc Blue (Hoechst 33342) from ThermoFisher Scientific (Santa Clara, CA), Salmonella typhimurium TA100 from Microbial Type Culture Collection and Gene Bank (MTCC, Chandigarh, India), thalidomide and DMEDA from Tokyo Chemical Industry (Tokyo, Japan), nitrocellulose membranes from MDI Membrane Technologies (Ambala. India), LMPA and LE agarose from Lonza (Rockland, ME, USA), γ-H2AX Alexa Fluor 488 from Biolegend (San Diego, CA, USA), and PAD-PARP antibody from Abcam (MA, USA). The 293T cell line was purchased from National Centre for Cell Culture (Pune, India) and authenticated by Short Tandem Repeat DNA profiling from Life Code Technology, Delhi, India. HepG2 cells were a generous gift from Dr. Soumya Sinha Roy at the CSIR Institute of Genomics and Integrative Biology, New Delhi. HUVEC was procured from HiMedia (Mumbai, India).

Wani T.H., Chakrabarty A., Shibata N., Yamazaki H., Guengerich F.P, & Chowdhury G. (2017). The Dihydroxy Metabolite of the Teratogen Thalidomide Causes Oxidative DNA Damage. Chemical research in toxicology, 30(8), 1622-1628.

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Protein Extraction from Aspergillus fumigatus

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A. fumigatus wet mat (1.5 g) with
or without any
treatment was ground in liquid nitrogen separately, and total protein
was isolated at 4 °C in chilled 50 mM sodium phosphate buffer
(pH 7.0) containing 2 mM EDTA (HiMedia, India), 0.2 mM dithiothreitol
(HiMedia, India), and 1 mM PMSF (HiMedia, India), for 3 h under constant
stirring. The extracted protein was then centrifuged at 12 000
rpm for 20 min at 4 °C. Trichloroacetic acid (5% of total volume)
was added to the supernatant. The precipitate was washed with chilled
acetone and dissolved in 6 M guanidium chloride (HiMedia, India).^{39 (link)}

Hoda S., Gupta L., Shankar J., Gupta A.K, & Vijayaraghavan P. (2020). cis-9-Hexadecenal, a Natural Compound Targeting Cell Wall Organization, Critical Growth Factor, and Virulence of Aspergillus fumigatus. ACS Omega, 5(17), 10077-10088.

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