Differentiation media bulletkits adipogenic

Manufactured by Lonza

Differentiation Media BulletKits-Adipogenic is a lab equipment product designed for the differentiation of adipogenic cells. It provides the essential components required for the in vitro differentiation of cells into adipocytes (fat cells).

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Lab products found in correlation

Sudan 3 staining, by Merck Group (3 mentions) Von kossa staining, by Abcam (2 mentions) Differentation media bulletkits osteogenic, by Lonza (1 mentions)

Close spelling variants of this product

Differentiation Media BulletKits Adipogenic differentiation media Adipogenic BulletKit Adipogenic media BulletKits

3 protocols using differentiation media bulletkits adipogenic

Osteogenic and Adipogenic Differentiation of HASCs

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To induce osteogenic differentiation, HASCs, harvested at passage #5 and with 60–70% confluency, were cultured in Differentation Media BulletKits-Osteogenic (Lonza), according to manufacturer’s instructions. The differentiation potential for osteogenesis was assessed by mineralization of calcium accumulation on von Kossa staining (Abcam, Bristol, UK), according to producer’s instructions; moreover, we determined changes in RT-PCR expression of specific genes, namely, Secreted Phospho-Protein 1, Bone Gamma-carboxyglutamate (Gla) Protein (BGLAP), Runt-related transcription factor 2 (RUNX2), Alkaline Phosphatase, Liver/bone/kidney (ALPL). To induce adipogenic differentiation, HASCs harvested as indicated above were cultured in Differentiation Media BulletKits-Adipogenic (Lonza). The potential for adipogenic differentiation was assessed by Sudan III staining (Sigma-Aldrich), according to the manufacturer’s instructions. Changes in the expression of specific genes, markers of adipogenic differentiation, such as Peroxisome Proliferator-Activated Receptor Gamma (PPARG), Lipo-Protein Lipase (LPL), Fatty Acid Binding Protein 4 (FABP4), were also determined by RT-PCR.

Romani R., Pirisinu I., Calvitti M., Pallotta M.T., Gargaro M., Bistoni G., Vacca C., Di Michele A., Orabona C., Rosati J., Pirro M., Giovagnoli S., Matino D., Prontera P., Rosi G., Grohmann U., Talesa V.N., Donti E., Puccetti P, & Fallarino F. (2015). Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3-dioxygenase1. Journal of Cellular and Molecular Medicine, 19(7), 1593-1605.

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Adipogenic Differentiation of HASCs

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HASCs, harvested as indicated above, were cultured in Differentiation Media Bullet Kits-Adipogenic (Lonza). Changes in the expression of specific genes, markers of adipogenic differentiation, such as Peroxisome Proliferator-Activated Receptor Gamma (PPARG), Lipo-Protein Lipase (LPL), and Fatty Acid Binding Protein 4 (FABP4) were determined by RT-q-PCR using specific primers (Table 1). The potential for adipogenic differentiation was assessed by Sudan III staining (Sigma-Aldrich), according to the manufacturer's instructions. Percentage of positive cell staining was recorded by measuring Sudan III-positive stained areas relative to a total selected area in five different images, using ImageJ software.

Romani R., Manni G., Donati C., Pirisinu I., Bernacchioni C., Gargaro M., Pirro M., Calvitti M., Bagaglia F., Sahebkar A., Clerici G., Matino D., Pomili G., Di Renzo G.C., Talesa V.N., Puccetti P, & Fallarino F. (2018). S1P promotes migration, differentiation and immune regulatory activity in amniotic-fluid–derived stem cells. European Journal of Pharmacology, 833, 173-182.

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Mesenchymal Stem Cell Differentiation

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Embryoid body (EB) formation was assessed by the method described by Valli A. et al. 27 To induce osteogenic differentiation, HASCs, harvested at passage 5 and with 60-70% confluency, were cultured in Differentiation Media BulletKits-Osteogenic (LONZA), according to manufacturer's instructions. The differentiation potential for osteogenesis was assessed by mineralization of calcium accumulation on von Kossa staining (Abcam), according to producer's instructions; moreover, we determined changes in RT-PCR expression of specific genes, namely, Secreted Phospho-Protein 1 (SPP1), Bone Gammacarboxyglutamate (Gla) Protein (BGLAP), Runt-related transcription factor 2 (RUNX2), Alkaline Phosphatase, and Liver/ bone/kidney (ALPL). To induce adipogenic differentiation, HASCs harvested as indicated above were cultured in Differentiation Media BulletKits-Adipogenic (LONZA). The potential for adipogenic differentiation was assessed by Sudan III staining (Sigma-Aldrich), according to the manufacturer's instructions. The changes in the expression of specific genes, markers of adipogenic differentiation, such as Peroxisome Proliferator-Activated Receptor Gamma (PPARG), Lipo-Protein Lipase (LPL), and Fatty Acid Binding Protein 4 (FABP4), were also determined by RT-PCR.

Romani R., Fallarino F., Pirisinu I., Calvitti M., Caselli A., Fiaschi T., Gamberi T., Matino D., Talesa V.N., Donti E., Puccetti P., Modesti A, & Magherini F. (2015). Comparative proteomic analysis of two distinct stem-cell populations from human amniotic fluid. Molecular bioSystems, 11(6).

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