Automated immunostainer
The Automated Immunostainer is a laboratory instrument designed for the automated processing of immunohistochemistry (IHC) and in situ hybridization (ISH) assays. The core function of this equipment is to automate the staining process, including antigen retrieval, primary antibody incubation, and chromogen detection, in a standardized and reproducible manner.
Lab products found in correlation
24 protocols using automated immunostainer
Immunostaining of BM biopsies for NK cells
Immunohistochemical Profiling of Epigenetic Markers in FFPE Tissue Microarrays
Immunohistochemical Profiling of Vascular Pathology
α-SMA and h-caldesmon stains were used to quantify the percentage of the fibrous rupture of the tunica media of the muscular arteries. α-SMA stains also evaluated the infiltration of myofibroblasts in the intima. CD45, CD3, and CD68 stains assessed the count of leukocytes, T lymphocytes, and macrophages per 10 high magnification (× 400) fields, respectively.
Lymph Node Immunohistochemistry Profiling
Comprehensive Evaluation of Acute Myeloid Leukemia
Standard PCR-based and cytogenetic analysis was performed at the Associated Regional and University Pathologists, Inc., ARUP Laboratories (Salt Lake City, Utah). Detection of the FLT3 TKD and IKD mutation was performed on isolated DNA using targeted fluorescent PCR primers for sequence amplification. The TKD products were cut using the EcoRV restriction enzyme and the resultant amplified ITD and TKD sequences were analyzed for base pair length on an ABI 3500xl genetic analyzer. A fragment of NPM1 exon 12 was also analyzed by targeted PCR amplification and interpreted using capillary electrophoresis. For cytogenetic studies, chromosomes were prepared from a nondiluted bone marrow aspirate collected in a heparinized syringe. The specimens were transported within 48 hours to ARUP laboratories. Each sample was cultured and suspended in metaphase. Giemsa-banded karyotyping was performed and interpreted with ISCN 2013.
Quantifying Liver Fibrosis and Cell Populations
GFAP Immunohistochemistry in PDHGG
Xenograft Tumor Analysis in NSG Mice
Immunohistochemical Analysis of T-ALL in Pediatrics
Comprehensive Immunohistochemistry Profiling
For every marker, positive cells were counted out of 10 non-overlapping randomly selected high-power microscopic fields (HPFs, 40×10), and the mean number of positively stained cells per HPF was recorded. The percentage of positive cells was calculated as the ratio between the mean number of stained cells per HPF and the mean number of total cells per HPF.
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