Guanabenz

Manufactured by Bio-Techne

Guanabenz is a laboratory reagent used in biological research. It is a synthetic compound with a molecular formula of C9H9Cl2N3. Guanabenz functions as an alpha-2 adrenergic receptor agonist, which can be utilized in various in vitro and in vivo experimental settings.

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Lab products found in correlation

Salubrinal, by Bio-Techne (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Antibiotic and antimycotic, by Thermo Fisher Scientific (2 mentions) Cholera toxin, by Merck Group (2 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions) Insulin, by Merck Group (2 mentions) Hydrocortisone, by Merck Group (2 mentions) Lapatinib was, by Selleck Chemicals (2 mentions) Cb 5083, by Cayman Chemical (2 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Thapsigargin, by Santa Cruz Biotechnology (1 mentions) Tnf α, by R&D Systems (1 mentions) Dulbecco s modified eagle s medium f12, by Thermo Fisher Scientific (1 mentions) Il 1β, by Merck Group (1 mentions) Cytochalasin d, by Merck Group (1 mentions) Methyl beta cyclodextrin mβcd, by Merck Group (1 mentions) Tnf α, by Merck Group (1 mentions)

Spelling variants of this product

Guanabenz acetate (3 citations)

4 protocols using guanabenz

Chondrocyte ER Stress Response

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Human primary chondrocytes (PC136121A1-C, Asterand Bioscience, Cambridge, Massachusetts) and
human chondrocyte cell line, C28/I2, were cultured in DMEM/F12 and DMEM mediums, respectively, which
were supplemented with 10% fetal bovine serum and antibiotics (Life Technologies, Grand Island, New
York). Cells were maintained at 37°C and 5% CO2 in a humidified incubator. After ten hours of
serum-free conditions, cells were incubated with 10 ng/ml TNFα (R&D Systems, Minneapolis,
Minnesota) in the presence and absence of 5 µM Salubrinal or 5 µM Guanabenz (TOCRIS
Bioscience, Ellisville, Missouri). One µM thapsigargin (Santa Cruz Biotechnology, Santa Cruz,
California) was used as a positive control (PC) for phosphorylation of eIF2α.

Hamamura K., Nishimura A., Iino T., Takigawa S., Sudo A, & Yokota H. (2015). Chondroprotective effects of Salubrinal in a mouse model of osteoarthritis. Bone & Joint Research, 4(5), 84-92.

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Breast Cancer Cell Line Culturing and Drug Treatment

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Cell lines were purchased from the American Type Culture Collection (ATCC). Human HER2+ breast cancer SKBR3 (HTB-30) cells were cultured in RPMI 1640 (Gibco) containing 10% fetal bovine serum with 0.1% antibiotic and antimycotic (Gibco). Human mammary epithelial MCF10A (CRL-10317) cells were cultured in Dulbecco’s modified Eagle’s medium/F12 containing 10% horse serum with 0.1% antibiotic and antimycotic (Gibco), hydrocortisone, cholera toxin, insulin (all from Sigma) and EGF (PeproTech Inc.). For drug treatments, cells were incubated with lapatinib was from (Selleck Chemicals, S1028), 250nM CB-5083 (Cayman Chemicals, 19311), and 15μM guanabenz (Tocris, 0885).

Singh N., Romick-Rosendale L., Watanabe-Chailland M., Privette Vinnedge L.M, & Komurov K. (2022). Drug resistance mechanisms create targetable proteostatic vulnerabilities in Her2+ breast cancers. PLOS ONE, 17(12), e0256788.

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Modulating eIF2α Phosphorylation on Src Activity

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Two types of proinflammatory cytokines, TNFα (Sigma; 10 ng/ml) and IL1β (Sigma; 1 ng/ml), were used. Salubrinal and guanabenz (both from Tocris Bioscience), inhibitors of eIF2α phosphatase, were used to test the effect of phosphorylation of eIF2α on Src activity [28] (link), [33] (link). We also used eIF2α siRNA and non-specific control (NC) siRNA (Origene). Cytochalasin D (Sigma; 1 µg/ml) was used to disrupt actin filaments. Methyl-beta-cyclodextrin (MβCD; Sigma; 10 mM) was used to extract cholesterol from the lipid rafts of the plasma membrane.

Wan Q., Xu W., Yan J.L., Yokota H, & Na S. (2014). Distinctive Subcellular Inhibition of Cytokine-Induced Src by Salubrinal and Fluid Flow. PLoS ONE, 9(8), e105699.

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Lapatinib, CB-5083, and Guanabenz on Breast Cancer

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Human HER2+ breast cancer SKBR3 cells were cultured in RPMI 1640 (Gibco) containing 10% fetal bovine serum with 0.1% antibiotic and antimycotic (Gibco). Human mammary epithelial MCF10A cells were cultured in Dulbecco's modified Eagle's medium/F12 containing 10% horse serum with 0.1% antibiotic and antimycotic (Gibco), hydrocortisone, cholera toxin, insulin (all from Sigma) and EGF (PeproTech Inc.). For drug treatments, cells were incubated with lapatinib was from (Selleck Chemicals, S1028), 250nM CB-5083 (Cayman Chemicals, 19311), and 15M guanabenz (Tocris, 0885).

Singh N., Romick-Rosendale L.E., Watanabe‐Chailland M., Vinnedge L.M.P., & Komurov K. (2021). Drug resistance mechanisms create targetable proteostatic vulnerabilities in Her2+ breast cancers.

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