Log In Sign up
The largest database of trusted experimental protocols

L ascorbate 2 phosphate

Manufactured by Fujifilm
Visit Supplier
Sourced in United States

L-ascorbate-2-phosphate is a chemical compound that serves as a raw material in various laboratory applications. It is a stable, water-soluble form of ascorbic acid (vitamin C). This compound is commonly used as a reducing agent and antioxidant in research and analytical procedures.

Automatically generated - may contain errors

Lab products found in correlation

Dexamethasone (dex), by Merck Group (3 mentions) Its premix, by BD (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Pet 1009030, by Sterlitech (1 mentions) Dmem with 4.5 g l glucose, by Thermo Fisher Scientific (1 mentions) Fibronectin, by BD (1 mentions) Transforming growth factor β1, by Thermo Fisher Scientific (1 mentions) Alcian blue, by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) 4.5 g l glucose, by Thermo Fisher Scientific (1 mentions) Rnalater, by Qiagen (1 mentions)

Close spelling variants of this product

Ascorbate-2-phosphate Ascorbate

6 protocols using l ascorbate 2 phosphate

1

Quantifying Multipotent Stromal Cell Colonies

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine the number of colony-forming unit fibroblast (CFU-F) colonies in the initial MNC population, freshly isolated UCB cells were seeded at low seeding density (1 × 105 MNCs/cm2) onto ECM and uncoated or fibronectin-coated TCP surfaces and cultured at 37 °C in growth media. Half media changes were performed every 3 days. After 1 month, the cells were fixed and stained with crystal violet to determine CFU-F number. To assess CFU osteoblast (CFU-OB) colony formation, CFU-F colonies were maintained for an additional 25 days in osteoblast differentiation medium (growth media supplemented with 100 nM dexamethasone (Sigma), 50 μM l-ascorbate-2-phosphate (Wako Chemicals, Richmond, VA, USA), and 10 mM glycerol 2-phosphate). CFU-OB colonies were counted after staining with von Kossa.
Wu J., Sun Y., Block T.J., Marinkovic M., Zhang Z.L., Chen R., Yin Y., Song J., Dean D.D., Lu Z, & Chen X.D. (2016). Umbilical cord blood-derived non-hematopoietic stem cells retrieved and expanded on bone marrow-derived extracellular matrix display pluripotent characteristics. Stem Cell Research & Therapy, 7, 176.
+ Open protocol
+ Expand
2

Murine Bone Stromal Cell Osteogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone derived mesenchymal stromal cells isolated from murine tibia and femora were cultured in αMEM supplemented with 20% (v/v) FCS, 2 mM l-glutamine, 1 mM sodium pyruvate, 10 mM HEPES buffer, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 µM l-ascorbate-2-phosphate (Wako Pure Chemical Industries, Richmond, VA, USA), 10 nM dexamethasone (RAH688A; Royal Adelaide Hospital, Adelaide, SA, Australia), and 4 mM KH2PO4 (Asia Pacific Specialty Chemicals Limited, Seven Hills, NSW, Australia). Cultures were stained with Alizarin red (A5533-25G; Sigma-Aldrich) to identify mineral deposits. Extracellular calcium (Ca2+) was measured by Calcium Arsenazo III (TR29226; Thermo Fisher Scientific) and normalized to DNA per well with a PicoGreen dsNDNA quantitation kit (P11496; Thermo Fisher Scientific). This analysis was conducted in triplicate for each mouse.
Arthur A., Nguyen T.M., Paton S., Klisuric A., Zannettino A.C, & Gronthos S. (2018). The osteoprogenitor-specific loss of ephrinB1 results in an osteoporotic phenotype affecting the balance between bone formation and resorption. Scientific Reports, 8, 12756.
+ Open protocol
+ Expand
3

Chondrocyte Differentiation in Biochamber

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Chondrocytes were applied to a custom double-diffusion biochamber with porous (10 µm pore diameter) polyester membrane (PET 1009030; Sterlitech, Kent, WA), which had been precoated with 5 µg/ml fibronectin (BD Bioscience, San Jose, CA), as previously described (11 (link)), at a density of 3.13 × 106 cells/cm2. The cells were cultured under the same O2 condition as in expansion with bioreactor medium (serum-free DMEM with 4.5 g/L glucose (Invitrogen, Grand Island, NY), containing 1% ITS-premix (BD Bioscience, San Jose, CA), 100 nM dexamethasone (Sigma-Aldrich), 37.5 µg/mL L-ascorbate-2-phosphate (Wako chemicals, Osaka, Japan), and 1% sodium pyruvate, 1% non-essential amino acid, 1% glutamax, and 1% Penicillin/Streptomycin (all from Invitrogen, Grand Island, NY)). The medium was changed every other day. After 3 weeks, cartilage sheets were removed from the biochamber and allowed to free float until 6.5 weeks. Punches (5 mm in diameter) were taken for the mechanical testing from each sheet. Combined GAG/DNA analysis was made in triplicate (3 mm diameter punches), as was collagen/collagen cross-link analysis. Histology was performed on a single neutral buffered formalin fixed 3 mm punch from each sheet. The sheets were made in duplicate from 4 donors.
Kean T.J., Mera H., Whitney G.A., MacKay D.L., Awadallah A., Fernandes R.J, & Dennis J.E. (2016). Disparate Response of Articular- and Auricular-derived Chondrocytes to Oxygen Tension. Connective tissue research, 57(4), 319-333.
+ Open protocol
+ Expand
4

Chondrogenic Differentiation of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were subjected to pellet culture as previously described38 (link) with minor modifications. Briefly, 1 × 106 cells in the growth medium were suspended in a 15-ml centrifuge tube (Becton Dickinson) and centrifuged at 430 × g for 5 min to generate a pellet. Next, 1 ml of chondrogenic medium consisting of Dulbecco’s modified Eagle medium/F12 containing 10% FBS, 10 ng/ml transforming growth factor-β1 (Peprotech, Oak Park, CA, USA), 1% ITS + 1 supplement (Merck KGaA), and 50 mM L-ascorbate-2-phosphate (Wako Pure Chemical) was gently added into the centrifuge tube. The differentiation medium was changed every 3 to 4 days during 4 weeks of differentiation cultivation, after which the pellets were fixed in 4% PFA, embedded in paraffin, and cut into 5 µm sections for histological analysis. Chondrogenic differentiation was determined by staining with 1% Alcian blue (Merck KGaA) solutions.
Toyota A., Shinagawa R., Mano M., Tokioka K, & Suda N. (2021). Regeneration in Experimental Alveolar Bone Defect Using Human Umbilical Cord Mesenchymal Stem Cells. Cell Transplantation, 30, 0963689720975391.
+ Open protocol
+ Expand
5

Isolation of Dental Pulp Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Incisors from adult BalbC mice were removed and their pulp exposed to enzymatic digestion with 3 mg/mL collagenase type I and 4 mg/mL dispase in PBS for one to two hours at 37°C with 5% CO2. The resulting solution was centrifuged at 200 × g for five minutes, the supernatant and enzymes removed and the remaining cells cultured in mesenchymal stem cell medium [19 (link)] containing alpha-modified Eagle’s medium (α-MEM) supplemented with 10% foetal bovine serum (FBS, Invitrogen, Mulgrave, Victoria, Australia), 1x GlutaMAX (Gibco, Mulgrave, Victoria, Australia), 100 μM L-ascorbate-2-phosphate (Wako, Neuss, Germany), 50 U/mL penicillin and 50 μg/mL streptomycin (Invitrogen), and dental pulp stem cells were allowed to adhere to the plastic base. Floating debris could subsequently be removed.
Ellis K.M., O’Carroll D.C., Lewis M.D., Rychkov G.Y, & Koblar S.A. (2014). Neurogenic potential of dental pulp stem cells isolated from murine incisors. Stem Cell Research & Therapy, 5(1), 30.
+ Open protocol
+ Expand
6

Chondrogenic Differentiation of TC-Expanded Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Aggregates were made from TC-expanded cells as described previously (6 (link)). Briefly, 250,000 cells were dispensed into several wells of a sterilized (autoclaved) polypropylene v-shaped bottom 96-well plate (Phenix, Hayward, CA), centrifuged at 590 RCF for 10 min, and placed in chondrogenic medium (serum-free DMEM with 4.5g/L glucose, containing 1% sodium pyruvate, and 1% penicillin/streptomycin [all from Invitrogen], 1% ITS-premix [BD Bioscience], 100 nM dexamethasone [Sigma-Aldrich], and 37.5 µg/mL L-ascorbate-2-phosphate [Wako chemicals]. The aggregates were cultured under the same oxygen condition as that used in expansion, and medium was changed every other day. At 21 days, the aggregates were collected and processed for combined GAG and DNA analysis (3 aggregates from each group), for combined collagen and collagen cross-links analysis (3 aggregates from each group), for lysyl oxidase (LOX) activity assay (3 aggregates from each group), for LOX gene expression analysis (3 aggregates from each group, stored in RNA Later® [Qiagen] at −80°C), and fixed in neutral buffered formalin for histology (1 aggregate from each group). Wet weights of all aggregates were taken after blotting excess media using filter paper.
Kean T.J., Mera H., Whitney G.A., MacKay D.L., Awadallah A., Fernandes R.J, & Dennis J.E. (2016). Disparate Response of Articular- and Auricular-derived Chondrocytes to Oxygen Tension. Connective tissue research, 57(4), 319-333.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!