Briefly, lung tissue sections were subjected to antigen retrieval and then blocked and incubated with 1:150 dilution of monoclonal anti Foxp3 antibody (eBioscience) and 1:300 dilution of anti‐rat IgG‐horseradish peroxidase thereafter (Servicebio). Images were captured using Olympus BX51 microscope (Olympus). Image‐Pro Plus 6.0 software (Media Cybernetics) was used to quantify the mean densities of Foxp3, which was stained brown in pixels at 200 × magnification.
Monoclonal anti foxp3 antibody
Manufactured by Thermo Fisher Scientific
Sourced in France
The Monoclonal anti Foxp3 antibody is a laboratory reagent used to detect and quantify the Foxp3 protein, a transcription factor that is a marker for regulatory T cells. The antibody can be used in various immunoassay techniques, such as flow cytometry, to identify and analyze Foxp3-expressing cells.
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2 protocols using monoclonal anti foxp3 antibody
1
Quantifying Foxp3+ Cells in Lung Tissue
2
Quantifying Tumor Immune Infiltrates
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Tumor biopsies were performed at inclusion and, if possible, at days 7, 14, and 38.
Histological analyses were performed on hematoxylin-eosin-saffron-stained tissue sections. Immunohistochemistry was performed using a polyclonal anti-CD3 antibody (dilution: 1:200) (DakoCytomation, Glostrup, Denmark, A0452) to detect T lymphocytes, a polyclonal anti-CD8 antibody (dilution: 1:100) (Abcam, Paris, France, ab4055) to detect cytotoxic T lymphocytes, and a monoclonal anti-Foxp3 antibody (dilution: 1:100) (eBioscience, San Diego, CA, 14-4776) to detect regulatory T lymphocytes. Staining was performed with a Leica BOND RXm Autostainer with the BOND Epitope Retrieval Solution for heat-induced epitope retrieval and Novolink Polymer Detection Systems (Leica Biosystems, Deer Park, IL). Then, TSA solution (Tyramide system amplification, Akoya Biosciences, Marlborough, MA) enabled fluorescent signal detection. Slides were digitalized with a NanoZoomer scanner (Hamamatsu, Massy, France) and digitally quantified with Calopix software (Tribvn, Châtillon, France).
Histological analyses were performed on hematoxylin-eosin-saffron-stained tissue sections. Immunohistochemistry was performed using a polyclonal anti-CD3 antibody (dilution: 1:200) (DakoCytomation, Glostrup, Denmark, A0452) to detect T lymphocytes, a polyclonal anti-CD8 antibody (dilution: 1:100) (Abcam, Paris, France, ab4055) to detect cytotoxic T lymphocytes, and a monoclonal anti-Foxp3 antibody (dilution: 1:100) (eBioscience, San Diego, CA, 14-4776) to detect regulatory T lymphocytes. Staining was performed with a Leica BOND RXm Autostainer with the BOND Epitope Retrieval Solution for heat-induced epitope retrieval and Novolink Polymer Detection Systems (Leica Biosystems, Deer Park, IL). Then, TSA solution (Tyramide system amplification, Akoya Biosciences, Marlborough, MA) enabled fluorescent signal detection. Slides were digitalized with a NanoZoomer scanner (Hamamatsu, Massy, France) and digitally quantified with Calopix software (Tribvn, Châtillon, France).
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