Zidovudine, abacavir, lamivudine, efavirenz, nevirapine, ritonavir, and nelfinavir were obtained from Sigma. Emtricitabine, tenofovir disoproxil fumarate, darunavir, dolutegravir and elvitegravir were obtained from MedChem Express. LysoSensor (DN160) was obtained from Fisher Thermo Scientific. Aβ1–40 and Aβ1–42 ELISA kits were obtained from Wako. ML-SA1 was obtained from Tocris Bioscience.
Dolutegravir
Manufactured by MedChemExpress
Sourced in United States
Dolutegravir is a chemical compound used as a laboratory tool. It functions as an integrase inhibitor, which is a type of antiretroviral medication. Dolutegravir is utilized in research settings to study its effects and potential applications.
Automatically generated - may contain errors
Lab products found in correlation
Darunavir, by MedChemExpress (3 mentions)
Ledipasvir, by MedChemExpress (2 mentions)
Daclatasvir, by MedChemExpress (2 mentions)
Elbasvir, by MedChemExpress (2 mentions)
Sofosbuvir, by MedChemExpress (2 mentions)
Etravirine, by MedChemExpress (2 mentions)
Raltegravir, by MedChemExpress (2 mentions)
Rilpivirine, by MedChemExpress (2 mentions)
Nelfinavir, by Merck Group (1 mentions)
Abacavir, by Merck Group (1 mentions)
Lamivudine, by Merck Group (1 mentions)
Nevirapine, by Merck Group (1 mentions)
Ritonavir, by Merck Group (1 mentions)
Elvitegravir, by MedChemExpress (1 mentions)
Zidovudine, by Merck Group (1 mentions)
Aβ1 40, by Fujifilm (1 mentions)
Aβ1 42 elisa kits, by Fujifilm (1 mentions)
Efavirenz, by Merck Group (1 mentions)
Ml sa1, by Bio-Techne (1 mentions)
Velpatasvir, by MedChemExpress (1 mentions)
5 protocols using dolutegravir
1
Evaluating Anti-HIV and Anti-Alzheimer's Compounds
2
High-Throughput ABCB1 Inhibition Assay
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[3H]-digoxin was purchased from Moravek Biochemicals (Brea, CA, USA). Abacavir, atazanavir, etravirine, lopinavir, maraviroc, rilpivirine, ritonavir, saquinavir, tenofovir DF, and zidovudine were obtained from the NIH AIDS Reagent Program. Asunaprevir, daclatasvir, darunavir, dolutegravir, elbasvir, grazoprevir, ledipasvir, sofosbuvir, and velpatasvir were acquired from MedChemExpress LLC (Middlesex County, NJ, USA). The model ABCB1 inhibitor CP100356 [76 (link)], the ATP bioluminescence assay kit, DMSO, Dulbecco’s Modified Eagle Medium, ethanol (EtOH), fetal bovine serum, nonessential amino acid solution, and penicillin–streptomycin solutions were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hanks’ balanced salt solution, William’s medium E containing L-glutamine (WME), and the bicinchoninic acid protein assay kit were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Krebs–Henseleit buffer was prepared as described by de Graaf et al. [31 (link)]. All other reagents were of analytical grade.
3
Synthesis and Characterization of Antiviral Compounds
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Stock solutions (100 mM) of dNTPs were obtained from GE Healthcare. [γ−32P] ATP (10 mCi/mL; 3000 Ci/mmol) was provided by Perkin Elmer. Synthetic oligonucleotides were obtained from Sigma and Integrated DNA Technologies. Nevirapine, efavirenz, etravirine and Rilpivirine were obtained from the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH. Rilpivirine and etravirine were supplied to the Program by Tibotec Pharmaceuticals, Inc. Doravirine, raltegravir (potassium salt) and dolutegravir were purchased from MedChem Express LLC (Monmouth Junction, NJ, USA).
4
Antiviral Drug Screening Protocol
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Acetaminophen (catalog number HY-66005), arbidol hydrochloride (HY-14904A), aspirin (HY-14654), atazanavir sulfate (HY-17367A), baloxavir marboxil (HY-109025), daclatasvir (HY-10466), darunavir (HY-17040), dolutegravir (HY-13238), efavirenz (HY-10572), elbasvir (HY-15789), etravirine (HY-90005), galidesivir (BCX4430, HY-18649A), ganciclovir (HY-13637), glecaprevir (HY-17634), hydroxychloroquine (HY-B1370), indomethacin (HY-14397), ledipasvir (HY-15602), lopinavir (HY-14588), losartan (HY-17512), nafamostat mesylate (HY-B0190A), nitazoxanide (HY-B0217), ombitasvir (HY-13997), oseltamivir (HY-13318), penciclovir (HY-17424), perindopril salt (HY-B0130A), raltegravir (HY-10353), remdesivir (GS-5734, HY-104077), rilpivirine (HY-10574), ritonavir (HY-90001), simeprevir (HY-10241), sofosbuvir (HY-15005), and β-d-N4-hydroxycytidine (HY-125033) were purchased from MedChemExpress (Monmouth Junction, NJ, United States). Amodiaquin dihydrochloride dihydrate (A2799), artesunate (A3731), chloroquine phosphate (PHR1258), mefloquine hydrochloride (PHR1705), peramivir trihydrate (SML2486), ribavirin (R9644), teicoplanin (T0578), and toremifene citrate salt (T7204) were purchased from Sigma-Aldrich (St, Louis, Missouri, United States). Favipiravir (T-705) was provided by the Toyama Chemical Co., Ltd. (Japan). The drug solutions were filtered through a 0.22 μM membrane and were stored at −80°C.
5
Erythrocyte Dolutegravir Exposure Assay
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Fresh Li-Heparin-anticoagulated blood samples were kindly provided by the blood bank of the University of Tübingen. The study is approved by the ethics committee of the University of Tübingen (184/2003 V). The blood was centrifuged at 120 x g for 20 min at 21 °C and the platelets and leukocytes-containing supernatant was disposed. Erythrocytes were incubated in vitro at a hematocrit of 0.4% in Ringer solution containing (in mM) 125 NaCl, 5 KCl, 1 MgSO 4 , 32 N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES; pH 7.4), 5 glucose, 1 CaCl 2 , at 37°C for 48 hours. Where indicated, erythrocytes were exposed for 48 hours to dolutegravir (MedChem Express, Princeton, USA). To test for an involvement of kinases, erythrocytes were exposed for 48 hours to a combination of dolutegravir and p38 kinase inhibitor SB203580 (Tocris bioscience, Bristol, UK), casein kinase inhibitor D4476 (Tocris bioscience, Bristol, UK) and protein kinase C inhibitor staurosporine (Enzo Life Sciences, Lörrach, Germany). The putative involvement of caspases was tested by the use of the pancaspase inhibitor zVAD (Enzo Life Sciences, Lörrach, Germany).
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