Mir 199a 5p inhibitor

The miR‐control, miR‐199a‐5p mimics and miR‐199a‐5p inhibitors were purchased from GenePharma. VSMCs were transfected with miR‐199a‐5p mimics, miR‐199a‐5p inhibitors or miR‐control (50nM) using Lipofectamine 2000 (11668027, Invitrogen) according to the manufacturer's instructions.

The miR control, miR-199a-5p mimics, and miR-199a-5p inhibitors were purchased from GenePharma (Shanghai, China). MSCs were transfected with miR control, miR-199a-5p mimic, or miR-199a-5p inhibitor (50 nM) using a Lipofectamine RNAiMAX Reagent Kit (2145966, Invitrogen, California, USA) according to the manufacturer’s instructions. The MSCs were cultured at 37 °C in a 5% CO₂ incubator following transfection and harvested 48 h later for further experiments. Transfection was performed at least three times.

LINC01123 pcDNA3.1 vector (1123-OE), c-Myc pcDNA3.1 vector (Myc-OE), and empty vector (vector) were subcloned into the expression vector pcDNA3.1 (Invitrogen, USA). MiR-199a-5p mimic, negative control oligonucleotides (mimics NC), miR-199a-5p inhibitor, negative control oligonucleotide (inhibitor NC), small interfering RNA of LINC01123 or c-Myc (si-1123, si-Myc), and scramble siRNA (si-NC) were purchased from GenePharma (Shanghai, China). The cells were seeded into six-well plates and cultured overnight until 70–80% confluence. Transfection was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instruction. The transfection efficiency was determined using qRT-PCR and Western blot. Sh-1123 and sh-NC lentivirus were purchased from GenePharma (Shanghai, China) and constructed into A549 cell lines for further in vivo experiments. The transfection efficiency was evaluated by fluorescence microscopy by calculating the percentage of fluorescein-labeled cells.