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Tov 21g cell line

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The TOV-21G cell line is a well-characterized human ovarian cancer cell line derived from a patient with a high-grade serous ovarian carcinoma. This cell line is commonly used in research to study the biology and treatment of ovarian cancer.

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4 protocols using tov 21g cell line

1

Protocols for Culturing OCCC Cell Lines

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The TOV-21G cell line was purchased from ATCC (RRID: CVCL_3613) and maintained in a 1:1 mixture of MCDB 105 medium and Medium 199 supplemented with 15% FBS. The OVISE cell line was a kind gift of Dr. R Zhang and was maintained in RPMI 1640 medium supplemented with 10% FBS and Penicillin/Streptomycin (Penicillin: 100 units/mL, Streptomycin: 0.1 mg/mL). The RMG1 cell line was a kind gift of Dr. R. Zhang and maintained in DMEM/F12 medium supplemented with 10% FBS and Penicillin/Streptomycin (Penicillin: 100 units/mL, Streptomycin: 0.1 mg/mL). All three OCCC lines were cultured at 37°C in a 5% CO2 humidified incubator.
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2

Endometriosis Tissue Sampling and Cell Lines

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Endometriosis tissue samples from the peritoneal wall were from three patients (mean age = 29.3 years) with American Society of Reproductive Medicine (ASRM) scores = 2. Patients were seen due to the presence of pelvic pain. Nondiseased endometrial tissue samples were from three nonmatched patients with endometriosis (mean age = 29.6 years). Tissue probes were from patients at a first diagnosis. All patients had menstrual cycles and no other diseases or prior treatment. In addition, one cell line TOV112D (ATCC: CRL-11731) from an endometrioid ovarian carcinoma and the TOV-21G cell line (ATCC: CRL-11730) from a clear cell ovarian carcinoma were chosen. All patients with endometriosis were scheduled for surgery independently of the study and were informed about the study prior to surgery. All tissue samples were sent to pathology for verification where only additional tissue from the same location was used for the present study. The study was approved by the Ethic Committee of the University Erlangen-Nuremberg (number 2567).
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3

Ovarian Clear Cell Carcinoma Cell Lines

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The TOV-21G cell line was purchased from ATCC (RRID: CVCL_3613) and maintained in a 1:1 mixture of MCDB 105 medium and Medium 199 supplemented with 15% FBS. The OVISE cell line was a kind gift of Dr. R Zhang and was maintained in RPMI1640 medium supplemented with 10% FBS and penicillin/streptomycin (penicillin: 100 units/mL, streptomycin: 0.1 mg/mL). The RMG1 cell line was a kind gift of Dr. R. Zhang and maintained in DMEM/F12 medium supplemented with 10% FBS and penicillin/streptomycin (penicillin: 100 units/mL, streptomycin: 0.1 mg/mL). All three OCCC lines were cultured at 37°C in a 5% CO2 humidified incubator.
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4

Culturing TOV21G and OVCAR3 Cell Lines

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The TOV21G cell line was purchased from ATCC (Manassas, VA, USA) and cultured in MCDB105 medium (Zhong Qiao Xin Zhou Biotechnology, Shanghai, China). The OVCAR3 cell line was purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China) and cultured in RPMI-1640 medium (Zhong Qiao Xin Zhou Biotechnology, Shanghai, China) supplemented with 10% FBS (Gibco, USA) and 1% penicillin/streptomycin (Invitrogen, USA). Both cell lines were maintained in an incubator at 37 °C with 5% CO2.
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