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Iron 2 chloride hexahydrate

Manufactured by Merck Group
Sourced in Germany

Iron (II) chloride hexahydrate is a chemical compound with the formula FeCl2·6H2O. It is a crystalline solid that appears green in color. The compound is soluble in water and is commonly used as a source of iron ions in various applications, including laboratory procedures and industrial processes.

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8 protocols using iron 2 chloride hexahydrate

1

Synthesis of Fe3O4@Au Nanoparticles

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Iron (II) chloride hexahydrate (97%), iron (III) chloride tetrahydrate (98%), hydrochloric acid (36.5–38.0%), and hydroxylamine hydrochloride (98%) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Sodium hydroxide (pure p.a., 98.8%), tetrachloroauric (III) acid (99.995% trace metals basis), and Arabic gum (powder) were bought from Avantor Performance Materials Poland S.A. (Gliwice, Poland). Essential oil used as a component of the reaction environment of Fe3O4@Au nanoparticles was bought in AromaLab (Warsaw, Poland).
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2

Synthesis of Functionalized Carbon Nanostructures

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SWCNTs (purity > 90%, ∼3 nm in outer diameter, <20 μm in length, special surface area = 400–600 m2 g−1), MWCNTs (purity > 95%, 20–40 nm in outer diameter, 5–15 μm in length, specific surface area = 40–300 m2 g−1), GE8 (8 nm), and GE12 (12 nm) used in this work were purchased from Uni-onward Corp. Tannic acid (TA), nickel(ii) chloride hexahydrate (NiCl2·6H2O), and iron(ii) chloride hexahydrate (FeCl2·6H2O) were purchased from Sigma-Aldrich. All chemicals are analytical grade and used as received without further purification.
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3

Synthesis of Iron Oxide Nanoparticles

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Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly-(ethylene glycol) (P123), hexadecyltrimethylammonium bromide (CTAB), mesitylene, tetraethyl orthosilicate (TEOS), iron(II) chloride hexahydrate, and ammonium hydroxide were purchased from Sigma-Aldrich. Hydrochloric acid (HCl) was purchased from J.T. Baker. Ammonium fluoride (NH4F) was purchased from Acros Organics. Iron(III) chloride tetrahydrate was purchased from Fisher Chemical.
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4

Analytical Workflow for Urine and Blood Metabolites

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2-aminothiazoline-4-carboxylic acid (ATCA) was purchased from Chem-Impex International (Wood Dale, IL). The internal standard, 2-aminothiazoline-4-carboxylic acid-13C, 15N (ATCA-13C, 15N), was obtained from Toronto Research Chemical, Inc. (North York, Canada). N-methyl-N-trimethylsilyl-trifluoroacetoamide (MSTFA), single-walled carbon nanotubes (0.7 – 1.3 nm in diameter and 1 μm in length), multi-walled carbon nanotubes (110 – 170 nm in diameter and 5 – 9 μm in length), carbon coated iron nanoparticles (25 nm average particle size), iron (II) chloride hexahydrate (FeCl3 · 6H2O), and iron (III) chloride tetrahydrate (FeCl2 · 4H2O) were purchased from Sigma-Aldrich (St. Louis, MO). Synthetic urine, Surine-, was obtained from Cerilliant (Round Rock, TX). Drug-free human urine was obtained commercially from UTAK Laboratories (Valencia, CA). Bovine blood was obtained from Quad Five (Ryegate, MT). The solvents, except for those used in the synthesis of Mag-CNT, were at least LCMS grade and all of the solvents were purchased from Sigma-Aldrich (St. Louis, MO) and Fisher Scientific (Hampton, NH). Deionized water was obtained from a Merck Millipore water purification system (Burlington, MA).
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5

Synthesis of Silica-Coated Iron Oxide Nanoparticles

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Iron(II)chloride hexahydrate (FeCl 2 .4H 2 O, ≥ 99%), 1-octadecene (ODE, technical grade 90%), oleic acid (OA, technical grade 90%), sodium citrate dihydrate (Na-citrate, ≥ 99%), tetramethylAmmonium hydroxide solution (TMAH, ACS reagent) and tetraethyl orthosilicate (TEOS, reagent grade 98%) were purchased from Sigma-Aldrich® (Schnelldorf, Germany). Cetyltrimethylammonium bromide (CTAB, ≥ 99%) and iron(III)chloride hexahydrate (FeCl 3 .6H 2 O, ≥ 99%) were bought from Acros Organics® (Geel, Belgium).
Trimethoxysilylpropyl-N,N,N-trimethylammonium chloride (TMAPS, in 50% methanol) and sodium oleate (> 97%) were purchased from abcr® (Karlsruhe, Germany) and Tokyo Chemical Industry (TCI, Japan), respectively. Ammonium hydroxide solution (25 wt%) was bought from Merck Life Sciences AS. The 3 dsDNA namely, T21, GM5 and GM6 were supplied by Biolegio B.V. (Nijmegen, Netherlands) and further details regarding the dsDNA can be found in the SI (Materials and Methods). All the chemicals were used as received without any further puri cation or modi cation. All solutions were prepared using distilled deionized water (MilliQ water), having a resistivity ~ 18.2 MΩ/cm at 25 ℃, taken from Simplicity® Millipore (Darmstadt, Germany) water puri cation system.
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6

Synthesis of Iron Oxide Nanoparticles

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Materials Ammonia (28 wt% in water), poly(5)oxyethylene-4-nonylphenyl-ether (Igepal Co 520), tetraethyl orthosilicate (TEOS, 99%), oleic acid (tech 90%), 1-octadecene (tech 90%) and iron (II) chloride hexahydrate (99.99%) were purchased from Sigma-Aldrich. Sodium hydroxide, ethanol, hexane, cyclohexane and other reagents were purchased from Alfa Aesar. All chemicals were used as received without further purification.
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7

Iron-based Composite Material Synthesis

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Iron (II) chloride hexahydrate (Merck Chemicals), Iron (III) Chloride hexahydrate (Merck Chemicals), 2-acetylpyridine (Sigma Aldrich), 4-hydroxybenzaldehyde (Sigma Aldrich), tetraethoxysilane (Sigma Aldrich), 3-aminopropyltriethoxysilane (Sigma Aldrich), dicyclohexylcarbodiimide (Sigma Aldrich), 4-dimethylaminopyridine (SAAR Chem), dimethylformamide (BDH limited), Sodium hydroxide (Merck Chemicals), rhodamine B (Sigma Aldrich), 25% ammonia solution (Merck Chemicals), absolute ethanol (Merck Chemicals) were used for this study.
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8

Spectrophotometric Determination of Iron

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CDs were freshly prepared by appropriate amount of solid in deionized water. Iron(iii) chloride hexahydrate (FeCl3·6H2O), iron(ii) chloride hexahydrate (FeCl2·6H2O), 1,10-phenanthroline, hydrochloric acid and ethanol were all from Merck (Darmstadt, Germany). For preparation of 1,10-phenanthroline solution, hydrochloric acid (0.5 mol L−1) was used. Deionized water was used throughout this study as solvent.
For Prussian blue assay, gallic acid (3,4,5-trihydroxybenzoic acid monohydrate) was purchased from Sigma-Aldrich (Taufkirchen, Germany). Analytical reagent grade potassium hexacyanoferrate(iii) (K3[Fe(CN)6]) and sodium dodecyl sulfonate (SDS) were purchased from Merck (Darmstadt, Germany).
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