Idelalisib (CAL-101, GS-1101; PI3Kδ inhibitor), duvelisib (IPI-145, INK1197; PI3Kγδ dual inhibitor), AS-604850 (PI3Kγ inhibitor) and ibrutinib (BTK inhibitor) were purchased from Selleckchem (Houston, TX) and all used at 1 μM concentration in most experiments. In some experiments inhibitors were used at 0.1 μM or 0.5 μM concentrations, as specifically indicated in the corresponding figure legend.
As 604850
Manufactured by Selleck Chemicals
AS-604850 is a laboratory equipment item. It is designed for specific laboratory functions. A detailed description of its core function is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Idelalisib, by Selleck Chemicals (2 mentions)
Duvelisib, by Selleck Chemicals (2 mentions)
Ibrutinib, by Selleck Chemicals (2 mentions)
Cxcl12, by Thermo Fisher Scientific (1 mentions)
Cxcl13, by R&D Systems (1 mentions)
Transwell chamber, by Corning (1 mentions)
Ccl21, by Thermo Fisher Scientific (1 mentions)
Pi 103, by Bio-Techne (1 mentions)
Ic87114, by Selleck Chemicals (1 mentions)
Tgx 221, by Bio-Techne (1 mentions)
3 protocols using as 604850
1
Inhibitors for Cell Signaling Research
2
Inhibitors for Cell Signaling Research
3
Chemotaxis Assay for Cell Migration
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CCL21 and CXCL12 were from Peprotech, and CXCL13 was purchased from R&D systems. Chemotaxis assays were carried out using Transwell chambers (5 µm pore size; CoStar) adding 100 µl cell suspension (5 x 106 cells/ml) in complete medium (RPMI/10% FCS/standard supplements) to the top chamber and indicated amounts of chemokine in the bottom chamber. After 2 h at 37°C, 7% CO2, the percentage of migrated cells was calculated by flow cytometry after comparing with a precalibrated bead standard (Sigma-Aldrich) and correcting for variations in input concentrations. The DOCK2 inhibitor CPYPP (Selleck) was used at 40 µM throughout the chemotaxis assay (15 (link)). The isoform-specific PI3K inhibitors TGX221 (0.1 µM final conc.; Tocris), PI-103 (1 µM; Tocris), AS604850 (1 µM; Selleck),and IC-87114 (0.5 µM; Selleck) were present throughout the chemotaxis assay.
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