Cells were treated as indicated, and 50 μM C11‐BODIPY Lipid Peroxidation Sensor (Thermo Fisher; Cat #D3861) was added and incubated for 1 h and protected from light. Excess C11‐BODIPY was removed from cells by washing the cells twice with PBS, and labelled cells were trypsinised, resuspended in PBS with 5% FBS. The fluorescence of C11‐BODIPY581/591 was analysed using a flow cytometer.
C11 bodipy lipid peroxidation sensor
Manufactured by Thermo Fisher Scientific
Sourced in United States
C11‐BODIPY Lipid Peroxidation Sensor is a fluorescent probe used to detect and quantify lipid peroxidation in biological samples. It functions by changing its fluorescence emission upon interaction with lipid peroxidation products.
Automatically generated - may contain errors
Lab products found in correlation
Ferric ammonium citrate, by Merck Group (1 mentions)
Recombinant mouse il 1β, by R&D Systems (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Gapdh antibody, by Proteintech (1 mentions)
Collagen 2, by Abcam (1 mentions)
Slc7a11 antibody, by Abcam (1 mentions)
P53 antibody, by Abcam (1 mentions)
Nqo 1 antibody, by Abcam (1 mentions)
Ho 1 antibody, by Proteintech (1 mentions)
Nrf2 antibody, by Proteintech (1 mentions)
Ferrostatin 1, by Selleck Chemicals (1 mentions)
Beta actin antibody, by Proteintech (1 mentions)
Erastin, by Selleck Chemicals (1 mentions)
2 protocols using c11 bodipy lipid peroxidation sensor
1
Lipid Peroxidation Sensor Assay
2
Ferroptosis-related Protein Regulation
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Ferric ammonium citrate (USA, 1185-57-5) was purchased from Sigma–Aldrich. Recombinant mouse IL-1β (Minneapolis, USA, # 401-ML) was purchased from R&D systems. Erastin (USA, S7242) and Ferrostatin-1 (USA, S7243) were purchased from Selleck. Reactive Oxygen Species Assay Kit (China, S0033) was purchased from Beyotime. C11 BODIPY Lipid Peroxidation Sensor (USA, D3861) was purchased from ThermoFisher. GPX4 (UK, ab125066, diluted 1:5000) antibody, P53 antibody (UK, ab131442, diluted 1:1000), SLC7A11 antibody (UK, ab175186, diluted 1:5000), ACSL4(UK, ab155282, diluted 1:10,000) antibody, Collagen II (UK, ab34712, diluted 1:200 for immunohistochemical staining) antibody, Collagen II (UK, ab185430, diluted 1:1000 for western blot) antibody, NQO-1 antibody (UK, ab80588, diluted 1:10,000), and Trx (UK, ab133524, diluted 1:10,000) antibody were purchased from Abcam. Nrf2 antibody (USA, 16396-1-AP, diluted 1:1000), HO-1 antibody (USA, 10701-1-AP, diluted 1:1000), Beta-actin antibody (USA, 66009-1-Ig, diluted 1:1000) and GAPDH antibody (USA, 60004-1-Ig, diluted 1:1000) were purchased from Proteintech.
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