Lc 20 analytical hplc system

The content of stilbenes was measured by HPLC as described [43 (link),59 (link)]. Briefly, the extracts were separated on Zorbax C18 column (column temperature 40 °C, 150 mm, 2.1-mm i.d., 3.5-lm part size, Agilent Technologies, Santa Clara, CA, USA) the on HPLC LC-20AD XR analytical system (Shimadzu, Kyoto, Japan). The mobile phase consisted of a gradient elution of 0.1% aqueous acetic acid (A) and acetonitrile (B). The gradient profile with a flow rate of 0.2 mL/min was: 0 min 0% B; 35 min 40% B; 40 min 50% B; 50 min 100% B and then eluent B until 60 min. The injected volume was 5 µL. Liquid chromatography-high-resolution mass spectrometry for quantification of all components was performed using a 1260 Infinity analytical system (Agilent Technologies, USA) as described [20 (link),59 (link)]. HPLC for quantification of all components was performed using LC-20 analytical HPLC system (Shimadzu, Japan) equipped with an SPD-M20A photodiode array detector, LC-20ADXR pump, Shim-pack XR-ODS II column and SIL-20ACXR auto sampler as described [46 (link)].

Total stilbene content was measured by HPLC as described [43 (link),44 (link)]. Briefly, the extracts were separated on Zorbax C18 column (150 mm, 2.1-mm i.d., 3.5-lm part size, Agilent Technologies, USA). The mobile phase consisted of a gradient elution of 0.1% aqueous acetic acid (A) and acetonitrile (B). The gradient profile with a flow rate of 0.2 mL/min was: 0 min 0% B; 35 min 40% B; 40 min 50% B; 50 min 100% B; and then eluent B for 60 min. Mass spectrometry was performed using a 1260 Infinity analytical system (Agilent Technologies, Santa Clara, CA, USA) for quantification of all components as described [43 (link)]. HPLC was performed using LC-20 analytical HPLC system (Shimadzu, Japan), equipped with an SPD-M20A photodiode array detector, LC-20ADXR pump, Shim-pack XR-ODS II column and SIL-20ACXR auto sampler for quantification of all components as described [44 (link)].