Log In Sign up
The largest database of trusted experimental protocols

Anti jak3

Manufactured by Merck Group
Visit Supplier

Anti-Jak3 is a laboratory equipment product developed by Merck Group. It is a selective inhibitor of the Janus kinase 3 (Jak3) enzyme, which plays a crucial role in the signaling pathways of various immune cells. The core function of Anti-Jak3 is to modulate the activity of Jak3, thereby affecting the regulation of immune responses.

Automatically generated - may contain errors

Lab products found in correlation

Anti actin c4, by Merck Group (1 mentions) Anti stat5, by Cell Signaling Technology (1 mentions) Anti cd4 gk1, by BD (1 mentions) Anti pstat5, by BD (1 mentions) Anti lck, by BD (1 mentions) Anti jak1, by Santa Cruz Biotechnology (1 mentions) Anti phospho jak3 tyr980 981, by Cell Signaling Technology (1 mentions) Anti stat3 c 20, by Santa Cruz Biotechnology (1 mentions) Anti phospho jak1, by Cell Signaling Technology (1 mentions) Anti α tubulin b 5 1 2, by Merck Group (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti phospho shp2, by Cell Signaling Technology (1 mentions) Anti phospho raf, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti phospho stat3, by Cell Signaling Technology (1 mentions)

2 protocols using anti jak3

1

Antibody Characterization for T Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies with the following specificities were used. For flow cytometry: anti–IL-7Rα (A7R34, eBioscience) and immunoglobulin G (IgG) isotype control (BD Pharmingen); for in vitro stimulation: anti-TCRβ (H57–957, BD Pharmingen) and anti-CD2 (RM2–5, BD Pharmingen); for Western blotting: anti-Jak1 (73/JAK1, BD Pharmingen and Cell Signaling Technology), anti-STAT5 (Cell Signaling Technology), anti-Lck (BD Pharmingen), anti-pSTAT5 (BD Pharmingen), anti-actin (C4, Millipore), and anti-Jak3 (Millipore); and for protein immunoprecipitation: anti-Jak1 (Santa Cruz Biotechnology), anti-Jak3 (Millipore), and anti-γc (Santa Cruz Biotechnology). Biotinylated anti-CD4 (GK1.5, BD Pharmingen) and anti-CD8 antibodies (53–6.7, BD Pharmingen) were used to deplete CD4+ and CD8+ T cells, respectively.
Katz G., Pobezinsky L.A., Jeurling S., Shinzawa M., Van Laethem F, & Singer A. (2014). T cell receptor stimulation impairs IL-7 receptor signaling by inducing expression of the microRNA miR-17 to target Janus kinase 1. Science signaling, 7(340), ra83.
+ Open protocol
+ Expand
2

Interleukin-21 Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human recombinant IL-21 was purchased from PeproTech EC. The antibodies used were anti-phospho-ERK1/2 (Thr 202/Tyr 204), anti-ERK1/2, anti-phospho-Raf (Ser 259), anti-phospho-SHP-2 (Tyr 542), anti-phospho-STAT3 (Tyr 705), anti-phospho-JAK1 (Tyr 1022/1023), and anti-phospho-JAK3 (Tyr 980/981) (Cell Signaling), anti-SHP-2 (C18), anti-Raf-1 (E10), anti-JAK1 (HR785), and anti-STAT3 (C20) (Santa Cruz), and anti-JAK3 (Millipore), and anti-αtubulin (B5-1-2) (Sigma).
Jin L., Nara H., Rahman M., Takeda Y., Araki A, & Asao H. (2018). Protein Tyrosine Phosphatase SHP-2 Is Involved in the Interleukin-21-Induced Activation of Extracellular Signal-Regulated Kinase 1/2. The Tohoku journal of experimental medicine, 244(3).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!