Rpmi 1640 culture solution

SMMC-7721 hepatocellular carcinoma cells (Shanghai Cell Institute of Chinese Academy of Sciences) were grown adhering to the walls of flasks, using RPMI-1640 culture solution (Gibco, Carlsbad, CA, USA) containing 10% newborn calf serum (Lanzhou Minhai Biology, Gansu, China), 100 U/ml penicillin and 100 µg/ml streptomycin in an incubator containing 95% O₂ and 5% CO₂ at 37°C.

PCa cell line LNCaP and normal prostate cell line RWPE-1 were purchased from the Shanghai Institute of Life Sciences. RPMI-1640 culture solution, fetal bovine serum (FBS), trypsin and penicillin-streptomycin double antibody were obtained from Gibco Co. (Grand Island, NY, USA). miR-200c primer sequences, miR-200c mimics (mimics), miR-NC control vector and miR-inhibitor (inhibitor) were all designed and synthesized by Shanghai Jema Corporation (Shanghai, China). RNA extraction TRIzol reagent and transfection kit Lipofectamine™ 2000 were purchased from Invitrogen. Thermo Fisher Scientific, Inc. (Waltham, MA, USA); Annexin V-FITC and cell counting kit-8 (CCK-8) kit were obtained from Shanghai Biyuntian Institute of Biotechnology (Shanghai, China). Transwell chamber was purchased from Corning Corporation (Corning, NY, USA); SYBR-Green PCR Master Mix kit was from Applied Biosystems (Foster City, CA, USA); the microplate reader SpectraMax M5 was from Shanghai Meigu Molecule (Shanghai, China); ABI 7900 PCR amplification instrument was purchased from Applied Biosystems; and flow cytometry CytoFLEX LX was from Beckman Coulter, Inc. (Brea, CA, USA).