The monoclonal human CAP1 antibody was previously described. Antibodies against GAPDH and GSK3β antibodies were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Antibodies against ERK1/2, phosphor-ERK1/2 (Thr202/Tyr204), phosphor-cofilin, and phospho-GSK3α/β (Ser21/Ser9) were from Cell Signaling Technology Inc. (Danvers, MA). Cofilin antibody was from Cytoskeleton Inc. (Denver, CO). Cell lysates were prepared for Western blotting similarly as previously described.
Phospho gsk3α β ser21 ser9
Manufactured by Cell Signaling Technology
Phospho-GSK3α/β (Ser21/Ser9) is a laboratory reagent that detects the phosphorylation of glycogen synthase kinase 3 alpha (GSK3α) and glycogen synthase kinase 3 beta (GSK3β) at serine 21 and serine 9, respectively. This product can be used for Western blotting applications to measure the relative phosphorylation levels of these proteins.
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Lab products found in correlation
2 protocols using phospho gsk3α β ser21 ser9
1
Immunoblotting analysis of signaling proteins
2
Western Blot Analysis of Cell Signaling Pathways
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The monoclonal human CAP1 antibody was previously described50 (link). Antibodies against GAPDH and GSK3 beta antibodies were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). E-cadherin antibody was from BD Biosciences (Franklin Lakes, NJ). Snail antibody was from Abcam (Cambridge, MA), and antibodies against ERK1/2, phosphor-ERK1/2 (Thr202/Tyr204), phosphor-cofilin, phospho-GSK3α/β (Ser21/Ser9), and FAK and phosphor-Y397 FAK were from Cell Signaling Technology Inc. (Danvers, MA). Cofilin antibody was from the Cytoskeleton Inc. (Denver, CO). Cell lysates were prepared for Western blotting similarly as previously described6 (link)51 (link).
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