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Anti human cd45ra percpcy5.5 clone hi100

Manufactured by BioLegend

Anti-human CD45RA-PERCPCy5.5 (Clone HI100) is a fluorochrome-conjugated antibody that specifically binds to the CD45RA isoform of the human CD45 protein. CD45RA is expressed on naive T cells and a subset of memory T cells. This product can be used for the identification and enumeration of these cell populations in flow cytometry applications.

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2 protocols using anti human cd45ra percpcy5.5 clone hi100

1

Multiparameter Flow Cytometry for T Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used in this study: anti-human CD45RA-PERCPCy5.5 (Clone HI100, Lot# B213966, Cat# 304107, Biolegend), anti-human CD127-Brilliant Violet 510 (Clone A019D5, Lot# B197159, Cat# 351331, Biolegend), anti-human CD4-APC-Cy7 (Clone OKT4, Lot# B207751, Cat# 317417, Biolegend), anti-human CCR6-PE (Clone G034E3, Lot# B203239, Cat# 353409, Biolegend), anti-human CD25-FITC (Clone BC96, Lot# B168869, Cat# 302603, Biolegend), anti-human CXCR3-Brilliant Violet 421 (Clone G025H7, Lot# B206003, Cat# 353715, Biolegend), anti-human CXCR5-AlexaFluor647 (Clone RF8B2, Lot# 5302868, Cat# 558113, BD Pharmingen), anti-human CD26-PE (Clone 2A6, Lot# 4301881, Cat# 12-0269-42, Thermo Fisher), and anti-human CD3E-Pacific Blue (Clone UCHT1, Lot# 4341657, Cat# 558117, BD Biosciences). All antibodies were validated by the manufacturer in human peripheral blood samples, used at a 1:200 dilution, and compared to isotype and no staining control samples.
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2

Multiparameter Flow Cytometry for T Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used in this study: anti-human CD45RA-PERCPCy5.5 (Clone HI100, Lot# B213966, Cat# 304107, Biolegend), anti-human CD127-Brilliant Violet 510 (Clone A019D5, Lot# B197159, Cat# 351331, Biolegend), anti-human CD4-APC-Cy7 (Clone OKT4, Lot# B207751, Cat# 317417, Biolegend), anti-human CCR6-PE (Clone G034E3, Lot# B203239, Cat# 353409, Biolegend), anti-human CD25-FITC (Clone BC96, Lot# B168869, Cat# 302603, Biolegend), anti-human CXCR3-Brilliant Violet 421 (Clone G025H7, Lot# B206003, Cat# 353715, Biolegend), anti-human CXCR5-AlexaFluor647 (Clone RF8B2, Lot# 5302868, Cat# 558113, BD Pharmingen), anti-human CD26-PE (Clone 2A6, Lot# 4301881, Cat# 12-0269-42, Thermo Fisher), and anti-human CD3E-Pacific Blue (Clone UCHT1, Lot# 4341657, Cat# 558117, BD Biosciences). All antibodies were validated by the manufacturer in human peripheral blood samples, used at a 1:200 dilution, and compared to isotype and no staining control samples.
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