αil 17a

Manufactured by Thermo Fisher Scientific

Sourced in United States

αIL-17A is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is an antibody that specifically binds to the cytokine interleukin-17A (IL-17A). The core function of αIL-17A is to facilitate the detection and quantification of IL-17A in various research and experimental settings.

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Lab products found in correlation

Facsaria 2, by BD (1 mentions) αifn γ, by BD (1 mentions) αcd11c, by Thermo Fisher Scientific (1 mentions) Mouse igg2a, by BioXCell (1 mentions) Allophycocyanin cy7, by BD (1 mentions) Efluor 450, by Thermo Fisher Scientific (1 mentions) Rmil 23, by R&D Systems (1 mentions) αifn γ, by Thermo Fisher Scientific (1 mentions) Methylated bovine serum albumin (mbsa), by Merck Group (1 mentions) Anti cd3 and anti cd28, by Thermo Fisher Scientific (1 mentions) Mycobacterium tuberculosis, by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Complete freund s adjuvant, by Merck Group (1 mentions) Ch223191, by Bio-Techne (1 mentions) α cd8, by Thermo Fisher Scientific (1 mentions) αcd62l, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) Brefeldin a, by Thermo Fisher Scientific (1 mentions) Ionomycin, by Merck Group (1 mentions)

Spelling variants of this product

αIL-17A-PE (eBio17B7, (2 citations)

6 protocols using αil 17a

XAGE-1b Specific T-cell Responses

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Following in vitro stimulation, CD4⁺ and CD8⁺ T cells were cultured for 14 days and then assessed for XAGE-1b specific T-cell responses. Cultures were collected and stimulated in the presence or in the absence of the XAGE-1b peptide pool (2 μM) and tested in a standard 4hr intracellular cytokine staining (ICS), using fluorescent-conjugated cytokine-specific antibodies αCD4 (BD Biosciences), αCD8 (BD Biosciences), αIL-17A (eBioscience) and αIFN-γ (BD Biosciences). The cytokine expression profile was then assessed by flow cytometry (FACSAria II, BD Biosciences).

Saito K., Nakayama E, & Valmori D. (2016). Immune Responses to the Cancer Testis Antigen XAGE-1b in Non Small Cell Lung Cancer Caucasian Patients. PLoS ONE, 11(3), e0150623.

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IL-17A Inhibition in Vaccinated Mice

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Purified monoclonal anti-mouse IL-17A (αIL-17A; eBioscience) was prepared using endotoxin-free PBS. Vaccinated mice were injected with 100 μg (100 μL i.p.) of αIL-17A 2 days before infection. The vaccinated and nonvaccinated control groups were injected with the nonspecific isotype control IgG (100 μg i.p.).

Yang L.Y., Zhou H., Yang Y., Tong Y.N., Peng L.S., Zhu B.H., Diao W.B., Zeng H., Sun H.W, & Zou Q.M. (2018). Protective effects of a nanoemulsion adjuvant vaccine (2C-Staph/NE) administered intranasally against invasive Staphylococcus aureus pneumonia. RSC Advances, 8(18), 9996-10008.

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Flow Cytometry Antibody Panel Protocols

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The following antibodies were obtained from eBioscience: Allophycocyanin-(αB220 [RA3-6B2], αCD11c [N418]), eFluor 450-(αCD45 [30-F11], αCD45.1 [A20]), eFluor 506-Fixable Viability, eFluor 780-Fixable Viability, FITC-(αGM-CSF [MP1-22E9], αMHCII [M5/114.15.2]), PE-αCD4 [RM4-5], PE-Cy7-(αCD44 [IM7], αCD11b [M1/70], αCD4 [RM4-5]), and PerCP-Cy5.5-(αIL-17A [eBio17B7], αCD11c [N418]). The following antibodies were obtained from BD Biosciences: Allophycocyanin-Cy7-(αIFNγ [XMG1.2], α-Ly6G [1A8]). PE-labeled MOG_38-49 tetramers were obtained from the NIH Tetramer Core Facility. rmIL-23 and rmIL-1α were obtained from R&D Systems. αGM-CSF (22E9.11), αIFNγ (XMG1.2), and αCD16/32 (2.4G2) were produced in house via hybridoma. 500µg of αGM-CSF or whole rat IgG (Sigma Aldrich) was administered i.p. every other day, beginning at time of immunization or T cell transfer, during blocking experiments. 100µg of αCD20 (clone 5D2, Genentech) or mouse IgG2a (clone C1.18.4, Bio X cell) was administered i.p. every other day, beginning at time of T cell transfer, in B cell depleting experiments.

Duncker P.C., Stoolman J.S., Huber A.K, & Segal B.M. (2017). GM-CSF promotes chronic disability in EAE by altering the composition of CNS myeloid cells, but is dispensable for disease induction. Journal of immunology (Baltimore, Md. : 1950), 200(3), 966-973.

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Monoclonal Antibody-Induced Cytokine Inhibition

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Monoclonal antibodies were applied to the vaccinated group to induce the functional inhibition of IFN-γ or IL-17A. Purified monoclonal anti-mouse IFN-γ (αIFN-γ; eBioscience; 16-7311-85) or anti-mouse IL-17A (αIL-17A; eBioscience; 16-7173-85) was prepared using endotoxin-free PBS. Two days before infection, vaccinated mice were injected with 100 μg (i.p.; 100 μL) of αIFN-γ, αIL-17A, or both. The vaccinated and nonvaccinated control groups were injected with the nonspecific isotype control IgG [100 or 200 μg (i.p.)] at doses comparable to those administered to the single inhibition group and the combined treatment group.

Yang L., Cai C., Feng Q., Shi Y., Zuo Q., Yang H., Jing H., Wei C., Zhuang Y., Zou Q, & Zeng H. (2016). Protective efficacy of the chimeric Staphylococcus aureus vaccine candidate IC in sepsis and pneumonia models. Scientific Reports, 6, 20929.

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Investigating AhR Signaling in T-Cell Activation

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The following materials were used: AHR agonist FICZ (6-formylindolo[3,2-b]carbazole, BIOMOL, Plymouth Meeting, PA, USA,); AHR antagonist CH223191 (1-Methyl-N-[2-methyl-4-[2-(2-methylphenyl)diazenyl]phenyl-1H-pyrazole-5-carboxamide, Tocris Bioscience, Ellisville, MO, USA); anti-CD3 and anti-CD28 (eBioscience, San Diego, CA, USA) benzo[b]fluoranthen, phorbol-12-myristate-13-acetate (PMA), ionomycin, methylated bovine serum albumin (mBSA), complete Freund’s adjuvant (CFA)with 1 mg/ml of Mycobacterium tuberculosis and RPMI 1640 medium (all from Sigma-Aldrich, St. Louis, MO, USA), α-IL-17a (eBioscience, anti-mouse IL-17A functional grade purified, Clone: eBioMM17F3).

Talbot J., Peres R.S., Pinto L.G., Oliveira R.D., Lima K.A., Donate P.B., Silva J.R., Ryffel B., Cunha T.M., Alves-Filho J.C., Liew F.Y., Louzada-Junior P, & de Queiroz Cunha F. (2018). Smoking-induced aggravation of experimental arthritis is dependent of aryl hydrocarbon receptor activation in Th17 cells. Arthritis Research & Therapy, 20, 119.

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Multiparametric Flow Cytometry Analysis

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Cells were labeled with fluorescence antibodies for mouse α-CD4, α-CD8, α-INF-γ, α-IL-2, α-IL-10, α-IL-4, α-IL-17A, α-CD44, and α-CD62L (eBioscience, CA). To assess immune responses after transplantation, total splenocytes or isolated CD4⁺ T cells were seeded on 48 well plates and stimulated in complete media for 4 hrs at 37°C with phorbol 12-myristate 13-acetate (PMA; 50 ng/ml; Sigma-Aldrich), Ionomycin (500ng/ml; Sigma-Aldrich) and Brefeldin A (eBioscience). Thereafter, cells were fixed, permeabilized and stained with respective antibodies at a concentration of 1–5 μg per 10⁶ cells. Flow cytometry measurements were performed on a FACS Canto II (BD Bioscience, CA, USA) and data were analyzed using FlowJo (FlowJo Software, OR, USA).

Krenzien F., Quante M., Heinbokel T., Seyda M., Minami K., Uehara H., Biefer H.R., Schuitenmaker J.M., Gabardi S., Splith K., Schmelzle M., Petrides A.K., Azuma H., Pratschke J., Li X.C., ElKhal A, & Tullius S.G. (2016). Age-dependent metabolic and immunosuppressive effects of Tacrolimus. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 17(5), 1242-1254.

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