The size and shape of PTXNRs were analyzed using a scanning electron microscope (Hitachi S4700 FESEM). The images were obtained at 10.0 kV accelerating voltage with 4.5 mm working distance and 40,000× magnification. The size distribution of NRs was analyzed using Fiji image processing software (Image J; Win 64 Java 1.8.0). The surface charge of PTXNRs was measured in DI water and phosphate-buffered saline (PBS) using zeta potential (Nano series Zetasizer, Malvern).
S 4700 fe sem
Manufactured by Hitachi
Sourced in Japan
The S-4700 FE-SEM is a field emission scanning electron microscope (FE-SEM) manufactured by Hitachi. It is designed for high-resolution imaging and analysis of a wide range of materials. The S-4700 FE-SEM utilizes a field emission electron source to produce a high-brightness, fine-diameter electron beam, enabling high-resolution imaging and analysis at low accelerating voltages.
Automatically generated - may contain errors
Lab products found in correlation
Em ace600, by Leica (3 mentions)
Nicolet is50, by Thermo Fisher Scientific (3 mentions)
Autosamdri 815, by Tousimis (2 mentions)
Sz4045, by Olympus (2 mentions)
Desk 5 hp tsc, by Denton (2 mentions)
Dma q800, by TA Instruments (2 mentions)
K alpha, by Thermo Fisher Scientific (2 mentions)
Zetasizer nano series, by Malvern Panalytical (1 mentions)
V 570 spectrophotometer, by Jasco (1 mentions)
6300d instrument, by Jasco (1 mentions)
Asap 2000, by Micrometrics (1 mentions)
Sta 6000, by PerkinElmer (1 mentions)
D8 advance, by Bruker (1 mentions)
X pert, by Philips (1 mentions)
Lf plus, by Ametek (1 mentions)
Skyscan 1172, by Bruker (1 mentions)
Zeta potential analyzer, by Malvern Panalytical (1 mentions)
Med 010, by Oerlikon Balzers (1 mentions)
Ir 460 spectrometer, by Shimadzu (1 mentions)
Em900 electron microscope, by Zeiss (1 mentions)
51 protocols using s 4700 fe sem
1
Characterization of Paclitaxel-Loaded Nanoparticles
2
Scanning Electron Microscopy of Stressed RBCs
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The RBCs in the blood samples from the different subgroups of applied external pressure were observed through scanning electron microscopy (SEM) using an S4700 FE-SEM (Hitachi, Tokyo, Japan) electron microscope. Centrifuged blood cells were fixed in phosphate-buffered (pH 7.2–7.4) 2.5% glutaraldehyde for 2 hours, washed twice in 0.1 M phosphate buffer (pH 7.2–7.4), and mounted on poly-L-lysine-coated glass slides. The glass slides were kept in a moist atmosphere for 1 hour, washed in phosphate buffer, post-fixed in 1% osmium tetroxide for 1 hour, rinsed in distillated water, and dehydrated in graded ethanol (50%, 70%, 90%, and 100%). After drying in air and covering with a gold layer by ion transfer, the samples underwent SEM analysis. The percentages of irreversibly changed cells were evaluated by counting 245 to 1455 cells in randomly chosen fields. The different cell shapes were identified using Bessis classification.[9 (link)]
3
Microscopic Analysis of Patterned Surfaces
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The morphologies and topographies of patterned surfaces were qualitatively and quantitatively analyzed using a scanning electron microscope (SEM; S-4700 FE-SEM, Hitachi, Tokyo, Japan) at 15 kV (Figure 2 A). Using ImageJ software (National Institutes of Health (NIH), Bethesda, MD, USA), the additively manufactured patterns on the surfaces in individual groups (μG-25, μG-19, μG-12, and μG-6) were qualitatively evaluated by creating topographical profiles (Figure 2 B). Moreover, experimental groups with four different intervals of microgroove patterns on scaffold surfaces were topographically and spatially characterized using confocal laser scanning microscope (CLSM; Carl Zeiss MicroImaging GmbH, Jena, Germany; Figure 2 C).
4
Characterization of Strontium Particles
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The size and morphology of selected Sr samples were observed through Field Emission-Scanning Electron Microscope (Hitachi S-4700 FE-SEM, Japan). Sr particles were centrifuged at 15 000 RPM for 10 sec, followed by removal of supernatant and resuspension with milli-Q water. Each particle suspensions were maintained under ice to reduce particle growth prior to microscopic observation. 1 µl of each sample was placed onto carbon tape-coated sample holder and dried at room temperature, followed by platinum sputtering of the dried samples for 30 sec. Microscopic observation of the sputtered Sr particles was visualized at 10–15 kV.
5
Comprehensive Materials Characterization Protocol
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The PXRD analysis was carried out on a Bruker D8 Advance instrument using Cu Kα (λ = 1.5406 Å) radiation at 2θ: 5° to 35°. Specific surface area, pore volume and pore size were measured by N2 adsorption–desorption at 77.360 K using a Micrometrics ASAP 2000 instrument. Before each measurement, samples were activated via supercritical CO2 drying or outgassing at room temperature for 3 h under vacuum conditions. Infrared spectra were collected in the range 400–4000 cm−1 on a JASCO 6300D instrument.
Thermogravimetric analyses (TGA) were performed on a PerkinElmer STA 6000 thermal analyzer. For this purpose, ca. 20 mg of sample was placed into an alumina crucible and heated in a continuous-flow of N2 with a heating ramp rate of 20 °C min−1 from 30 °C to 900 °C. The scanning electron micrographs were taken on a Hitachi S-4700 FE-SEM. The UV-vis spectra were recorded on a JASCO V-570 spectrophotometer.
Thermogravimetric analyses (TGA) were performed on a PerkinElmer STA 6000 thermal analyzer. For this purpose, ca. 20 mg of sample was placed into an alumina crucible and heated in a continuous-flow of N2 with a heating ramp rate of 20 °C min−1 from 30 °C to 900 °C. The scanning electron micrographs were taken on a Hitachi S-4700 FE-SEM. The UV-vis spectra were recorded on a JASCO V-570 spectrophotometer.
6
Characterization of Biomaterial Scaffolds
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The scaffolds were prepared by sputter coating with a 5 nm thick coating of Au/Pd for field emission scanning electron microscope (FESEM; Hitachi S-4700 FE-SEM). Fiji [37] (link) was used for image analysis. The surface chemistry of the scaffolds was characterized using Attenuated Fourier Transform Infrared Spectroscopy (ATF-FTIR, Thermo Scientific, Nicolet iS50) with a deuterated triglycine sulfate detector element. The measurements range of 400–4000 cm−1 at a resolution of 4 cm−1 with 256 scans was used. The mechanical properties of the scaffolds were determined by using a dynamic mechanical analyzer (TA Instruments, DMA Q800) under uniaxial strain ramp at isothermal conditions (37 °C). The Young's modulus was determined from the linear region of the stress-strain plot, uniaxial stiffness was determined from the force-displacement curve. The modulus of toughness and modulus of resilience were calculated from the area under the curve and area under the linear region of the stress-strain curve respectively.
7
Characterizing PCL/B3 Glass Scaffolds
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Optical microscopic images were used to measure the filament width and pore size with at least five measurements and the results were reported as mean ± standard deviation. Samples were sputter coated with gold/palladium (Au/Pd) for 60 s before performing scanning electron microscopy (SEM). SEM (Hitachi S-4700 FESEM, Hitachi Co., Tokyo, Japan) images were taken to evaluate the surface morphology of the scaffolds, internal structure of the filaments, and formation of hydroxyapatite-like material on the scaffold surface. Scans were run from 20 values ranging from 10° to 80° using Cu Ka radiation (X = 0.154056 nm) for X-ray diffraction (XRD) analysis (Philips X-Pert, Westborough, MA) on the as-received PCL, as-printed PCL/B3 glass scaffold, and the scaffold after a-MEM immersion to determine the changes in the crystalline/amorphous nature of the material.
8
Tensile Strength of Y-TZP Adhesive
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At a 0.5 mm/min crosshead speed, the adhesive interface of each specimen was loaded with a jig of the universal testing machine (LF-plus, AMETEK Inc., Largo, FL, USA) until failure occurred. The failure modes were observed under a stereomicroscope (45x). The resin bonding on the Y-TZP and fractured surfaces was examined using a scanning electron microscope (SEM; S-4700 FESEM, Hitachi, Tokyo, Japan) at 600x magnification and 10 kV accelerating voltage.
9
Morphological Analysis of Synthetic n-ZnO
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The morphology of the standard (R) and the synthetized n-ZnO particles was analyzed using a Hitachi S-4700 FE-SEM (Hitachi, Krefeld, Germany) operating with a BSE detector under low vacuum conditions. The samples of n-ZnO were placed on the adhesive carbon discs, without any coating. The size of nanoparticles were determined from SEM images (for samples B, C and D, it was their diameter).
10
Characterization of Microgroove Patterns in PCL Scaffolds
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For the qualitative analyses of angulated microgrooves and surface roughness, microgroove patterns on longitudinal architectures in the PCL scaffold were morphologically characterized using the SEM (Figure 2 ) at 15 kV (S-4700 FE-SEM, Hitachi, Japan). The spatial topographical evaluations were performed for measurements of groove distance (25.40 μm; Figure 3 D–F) and angular patterns against the reference direction, which was designed by the CAD for ligament architectures using the topography analysis of the confocal laser scanning microscope (CLSM; Carl Zeiss MicroImaging GmbH, Jena, Germany). The PCL-casted ligament scaffolds were volumetrically and cross-sectionally characterized using micro-CT (SkyScan 1172, Bruker-microCT, Knotich, Belgium), which provide 3D reconstructive and digitally-sectioned images and set to scan with an 11.55 μm3 voxel size under a 40 kV source voltage and a 200 μA source current.
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