Polyvinyl alcohol mw 2000 pvoh

Poly(ethylene glycol) diacrylate (M_n 700) (PEG₇₀₀DA), 2-aminoethyl methacrylate hydrochloride (AEM), diphenyl (2,4,6-trimethylbenzoyl)-phosphine oxide (TPO), thiol modified CpG 1826 (C6-S-S-C6-tccatgacgttcctgacgtt), dithiothreitol (DTT), sucrose, and DNase, RNase free sterile water were purchased from Sigma-Aldrich. Tetraethylene glycol monoacrylate (HP₄A) was synthesized in house. Cysteine modified OVA_257–264 (CSIINFEKL) were purchased from Peptide 2.0. Trifluoroacetic acid, methanol, dimethyl sulfoxide (DMSO), PTFE (polytetrafluoroethylene) syringe filters (13 mm membrane, 0.22-μm pore size), HPLC grade water, and acetonitrile were obtained from Fisher Scientific. Conventional filters (2 μm) were purchased from Agilent Technologies, and poly(vinyl alcohol) (M_w 2000) (PVOH) was purchased from Acros Organics. N-Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) was purchased from Thermo Scientific. Ortho-pyridyldisulfide-PEG-N-hydroxylsuccinimide ester (NHS-PEG (2k)-OPSS) was purchased from Creative PEGworks. PRINT molds (80 nm × 320 nm) were obtained from Liquidia Technologies. DNA grade NAP-10 columns were purchased from GE Healthcare. RPMI1640 medium, penicillin and streptomycin, L-glutamine, and fetal bovine serum (FBS) were all from Life Technologies.

Poly(ethylene glycol) diacrylate (M_n 700) (PEG₇₀₀DA), 2-aminoetheyl methacrylate hydrochloride (AEM), and Diphenyl (2,4,6-trimethylbenzoyl)-phosphine oxide (TPO) were from Sigma-Aldrich. Tetraethylene glycol monoacrylate (HP₄A) was synthesized in-house as previously described (35 ). Thermo Scientific maleimide-terminated Dylight 650 and Dylight 488, PTFE syringe filters (13mm membrane, 0.220 µm pore size), dimethylformamide (DMF), triethanolamine (TEA), pyridine, sterile water, borate buffer (pH 8.6), Dulbecco’s 1× phosphate buffered saline (DPBS) (pH 7.4), acetic anhydride and methanol were obtained from Fisher Scientific. Methoxy-PEG(5k)-succinimidyl carboxy methyl ester (mPEG_5k-SCM) was purchased from Creative PEGWorks. Conventional filters (2 µm) were purchased from Agilent and polyvinyl alcohol (Mw 2000) (PVOH) was purchased from Acros Organics. Liquidia Technologies kindly provided the 80 nm × 320 nm PRINT molds used in these studies.

Poly(ethylene glycol) diacrylate (Mw 700) (PEG700DA), 2-aminoethyl methacrylate hydrochloride (AEM), diphenyl (2,4,6-trimethylbenzoyl)-phosphine oxide (TPO), and sucrose were purchased from Sigma-Aldrich. Thermo Scientific Dylight 488 maleimide, dimethylformamide (DMF), triethylamine (TEA), pyridine, borate buffer (pH 8.6), acetic anhydride, and methanol were obtained from Fisher Scientific. Conventional filters (2 µm) were purchased from Agilent and poly(vinyl alcohol) (Mw 2000) (PVOH) was purchased from Acros Organics. PRINT molds (80 nm×80 nm×320 nm) were obtained from Liquidia Technologies. Tetraethylene glycolmonoacrylate (HP4A) was synthesized in-house as previously described.²⁶ Methoxy-PEG(5k)-succinimidyl carboxy methyl ester (mPEG5k-SCM) was purchased from Creative PEGWorks. Typsin, DPBS, and cell culture media were purchased from Gibco.