System gold hplc system

Manufactured by Beckman Coulter

Sourced in Germany, United States

The System Gold HPLC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative separations. It features a modular design and can be configured with various components, including pumps, detectors, and autosamplers, to meet specific analytical requirements. The system provides precise control of mobile phase flow, gradient formation, and sample injection, enabling accurate and reproducible chromatographic analysis.

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Lab products found in correlation

Pa100, by Thermo Fisher Scientific (1 mentions) Oligodeoxynucleotides, by Integrated DNA Technologies (1 mentions) Hplc solvents, by Thermo Fisher Scientific (1 mentions) Cary 100 bio uv vis spectrometer, by Agilent Technologies (1 mentions) Model 168, by Beckman Coulter (1 mentions) Nuclease p1 np1 from penicillium citrinum, by Merck Group (1 mentions) J 810 spectropolarimeter, by Jasco (1 mentions)

Close spelling variants of this product

System Gold HPLC Beckman System Gold HPLC Gold system HPLC system

5 protocols using system gold hplc system

Substrate Quantification via HPLC

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Example 5

For substrate quantification (glucose, glycerol, acetate, ethanol, and xylose) an ion exchange chromatography was applied. We used the System Gold HPLC system composed of the pump LC-126, the autosampler LC-508, the UV detector LC-166, (all Beckmann Coulter, Krefeld, Germany), the Jetstream 2 Plus column oven (Knauer, Berlin, Germany), and the refractive index detector LCD 201 (Gynkotek, Munich, Germany). The applied column was the Organic Acid Resin (Chromatographie Service GmbH, Langerwehe, Germany) with a length of 30 cm and a diameter of 8 mm. The running buffer was 5 mM sulfuric acid, which was pumped isocratically with a flow rate of 0.8 mL/min at a temperature of 50° C. 5 μL of the sample were injected.

US10907180B2. Extracellular production of designer hydroxyalkanoyloxy alkanoic acids with recombinant bacteria (2021-02-02). Rheinisch-Westfaelische Technische Hochshule Aachen (RWTH) [DE]. Inventors: Lars M. Blank [DE], Till Tiso [DE], Andrea Germer [DE].

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Quantification of Aromatic Metabolites

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After extracellular aromatics were identified using LC-MS with a PFP column (see supporting information), routine analysis and quantification of aromatics were performed using an Ultra AQ C18 5-μm column (Restek) attached to a System Gold HPLC system (Beckman Coulter) with running buffers described in Fig. S11A. The eluent was analyzed for light absorbance between 191 and 600 nm, and absorbances at 280 nm were used for quantification of aromatic metabolites by comparing peak areas with those of standards (retention times of measured metabolites are shown in Figs. S4 and S11B).

Kontur W.S., Bingman C.A., Olmsted C.N., Wassarman D.R., Ulbrich A., Gall D.L., Smith R.W., Yusko L.M., Fox B.G., Noguera D.R., Coon J.J, & Donohue T.J. (2018). Novosphingobium aromaticivorans uses a Nu-class glutathione S-transferase as a glutathione lyase in breaking the β-aryl ether bond of lignin. The Journal of Biological Chemistry, 293(14), 4955-4968.

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Enzymatic Synthesis and Purification of 2-5A

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2–5A (p₃(A2′p)_nA, where n = 1 to ≥3) was enzymatically synthesized from ATP using hexahistidine-tagged and purified recombinant porcine 42-kDa 2-5A synthetase (OAS1). Individual 2-5A oligomers were purified (>95% purity) using a Dionex PA100 (22 mm × 250 mm) semi-preparative column interfaced with a Beckman system gold HPLC system under the control of 32-Karat workstation [62 (link)]. The 5’-triphosphorylated triadenylate, p₃A2’p5’A2’p5’A, was used for RNase L activation. Biotinlyation of 2-5A was performed using periodate chemistry as described earlier [30 (link), 44 (link)].

Drappier M., Jha B.K., Stone S., Elliott R., Zhang R., Vertommen D., Weiss S.R., Silverman R.H, & Michiels T. (2018). A novel mechanism of RNase L inhibition: Theiler's virus L* protein prevents 2-5A from binding to RNase L. PLoS Pathogens, 14(4), e1006989.

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HPLC Analysis of Oligodeoxynucleotide Modifications

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Oligodeoxynucleotides were purchased from Integrated DNA Technologies, Inc. (IDT) (Coralville, Iowa). Nuclease P1 (NP1) from Penicillium citrinum, was from Sigma (St. Louis, MO). HPLC solvents were from Fisher (Fair Lawn, NJ). HPLC separation and analysis were carried out on System Gold HPLC system with a binary gradient Model 125 pump and a Model 168 diode array detector (Beckman Coulter, Inc., Fullerton, CA). An X-Bridge column (C18, 4.6 × 75 mm, 2.5 μm, 135 Å) from Waters Corporation (Milford, MA) was used for reverse-phase HPLC. UVB (280-320 nm) irradiation was carried out with two Spectroline XX-15B UV 15W tubes (312 nm) with peak UV intensity of 1150 μW/cm² at 25 cm filtered through a Longlife filter glass from Spectronics Corporation (Westbury, NY). CD experiments were carried out on a J-810 spectropolarimeter (Jasco). UV melting curves were obtained on a Cary 100 Bio UV-VIS Spectrometer (Varian).

Lu C., Smith-Carpenter J.E, & Taylor J.S. (2018). Evidence for Reverse Hoogsteen Hairpin Intermediates in the Photocrosslinking of Human Telomeric DNA Sequences. Photochemistry and photobiology, 94(4), 685-697.

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HPLC Analysis of Radiolabeled [68Ga]Ga-FAPI-46

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HPLC analysis of radio chemical identity (Table 1) was conducted using a System Gold HPLC System (Beckman Coulter, USA) equipped with an UV detector set to 220 nm and HERM LB 500 γ-detector (Berthold Technologies GmbH & Co. KG, Germany). As the stationary phase a Chromolith HR RP-18 100 × 3 mm HPLC-column (Merck KGaA, Germany) was used. As the mobile phase a gradient of solvents A (H₂O containing0.1% TFA) and B (acetonitrile containing 0.1% TFA); was used (0–8 min 0–100% B; flow rate: 1.0 mL/min).
The retention time of [⁶⁸Ga]Ga-FAPI-46 was compared to that of the non-active [^natGa]Ga-FAPI-46 reference standard and had to be within ± 10% of the standard relative retention time (RRT).

Alfteimi A., Lützen U., Helm A., Jüptner M., Marx M., Zhao Y, & Zuhayra M. (2022). Automated synthesis of [68Ga]Ga-FAPI-46 without pre-purification of the generator eluate on three common synthesis modules and two generator types. EJNMMI Radiopharmacy and Chemistry, 7, 20.

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