Nanostar u

Manufactured by Bruker

Sourced in Germany

The Nanostar U is a compact X-ray scattering system designed for materials characterization. It offers small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS) capabilities for analyzing the structure of materials at the nanometer scale.

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V ntec 2000 detector, by Bruker (1 mentions)

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Nanostar (55 citations) Nanostar U diffractometer (3 citations) NANOSTAR U SAXS instrument (2 citations)

6 protocols using nanostar u

SAXS Analysis of Peptide Coacervates

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Sample was prepared by dissolving 5.0 mg of lyophilized GY-23 peptide in 100 µL of 10 mM acetic acid (pH 3.3). Coacervation was induced by mixing of the peptide stock with the coacervation buffer (50 mM Tris-HCl, pH 7.0 buffer, containing 1 M NaCl) in 1 to 5 volume ratio. Coacervate-rich phase was collected by centrifugation (13,000g for 5 min at 25 °C) and transferred into a 1.5 mm quartz capillary together with some supernatant to avoid drying. The position of the capillary was then specifically aligned to hit the coacervate-rich phase.
SAXS measurements were performed on a Bruker Nanostar U (Bruker AXS, Karlsruhe, Germany) connected to a sealed-tube Cu anode X-ray source operating at 50 kV and 600 μA (Incoatec IμSCu, Geest-hacht, Germany). A Göbel mirror was used to convert the divergent polychromatic X-ray beam into a focused beam of monochromatic Cu Kα radiation (λ = 0.154 nm). The beam size was 0.3 mm. A sample to detector distance of 1077 mm gave the q-range 0.07 < q < 2.9 nm⁻¹. The 2D SAXS patterns were acquired within 1 h using a VÅNTEC-2000 detector (Bruker AXS, Karlsruhe, Germany) with an active area of 140 × 140 mm² and a pixel size of 68 μm.
The samples were measured in 1.5 mm quartz capillaries. The scattering curves were plotted as a function of intensity, I vs. q. Scattering from the corresponding buffer was subtracted as background from all samples.

Gabryelczyk B., Cai H., Shi X., Sun Y., Swinkels P.J., Salentinig S., Pervushin K, & Miserez A. (2019). Hydrogen bond guidance and aromatic stacking drive liquid-liquid phase separation of intrinsically disordered histidine-rich peptides. Nature Communications, 10, 5465.

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Bone Mineral Crystal Analysis via SAXS

Cited 1 time

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Three representative femora samples per experimental group were longitudinally sectioned from the mid-diaphyseal region to a uniform thickness (300 μm) and then set in epoxy (AstroChem 1119). Two-dimensional SAXS spectra were then acquired on a Bruker Nanostar-U (Bruker, Switzerland) at four selected points distributed around the cross-section. The elastic scattering of x-rays at a nanoscale allows for the evaluation of the relationship between scattered intensity (I), scattering vector (q) and azimuthal angle (ψ). This provides an estimation of thickness, orientation and shape of mineral crystals in bone; the scattering vector q is given by 4sin(θ)/λ (λ is the wavelength of x-rays (0.154 nm) and θ is the angle between the x-ray beam and detector).^{(33 (link))} The thickness of mineral crystals was calculated, following published literature.^{(33 (link))} In brief, the thickness of the mineral particles was based on a calculation of surface to volume ratio with the equation (4 J/πP). Kratky plots (q²I vs. q), were used to evaluate the area under the curve (J), and the Porod constant P was calculated for every 2D spectrum from regions where Iq⁴ was constant, based on Porod’s law (P = Iq⁴). To calculate the orientation of the crystals (ρ), I(ψ) versus ψ (0 < ψ < 2π) was plotted.

LLabre J.E., Trujillo R., Sroga G.E., Figueiro M.G, & Vashishth D. (2021). Circadian rhythm disruption with high-fat diet impairs glycemic control and bone quality. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(9), e21786.

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SAXS Characterization of Glycopolymers

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Small-angle X-ray
scattering (SAXS) measurements were taken on a Bruker Nanostar U instrument
in the high-resolution configuration (Brookhaven National Laboratory,
Upton, NY). The wavelength of the beam is 0.15418 nm with a Cu rotating
anode source. The nominal distance from sample to detector (Vantec
2000 area detector) was 1.1 m, and the actual distance was calibrated
with silver behenate before the measurements. Deprotected glycopolymer
solutions were prepared at the indicated concentrations and loaded
into quartz capillaries (diameter of 0.1 mm). The capillary was fixed
in the sample holder, and scattering data for each sample were collected
for 18 h. SAXS data were analyzed using the SasView small-angle scattering
analysis software package (http://www.sasview.org/).

Huang H., Rodolis M.T., Bhatia S.R, & Sampson N.S. (2017). Sugars Require Rigid Multivalent Displays for Activation of Mouse Sperm Acrosomal Exocytosis. Biochemistry, 56(22), 2779-2786.

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Multimodal Characterization of Nanostructures

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SAXS measurements were
performed on beamline 12-ID-B at the Advanced Photon Source using
a photon energy of 14 keV and a detector distance of 3.6 m (leading
to a q range of 0.002–0.52 Å^–1, the beam size is 30 μm × 200 μm) or on a lab-scale
X-ray setup (Bruker Nanostar U) at the Center for Functional Nanomaterials
of Brookhaven National Laboratories with a q range
of 0.0048–0.2 Å^–1. USAXS experiments
were performed on the Bonse/Hart camera at the LIONS laboratory in
CEA Saclay in France, covering a q range of 0.0003–0.09
Å^–1. Small angle neutron scattering (SANS)
experiments were carried out on the GP-SANS beamline at HFIR at the
Oak Ridge National Laboratory or the NGB 30m beamline at National
Institute of Standards and Technology. The VSANS data were collected
at the KWS-3 beamline at Heinz Maier-Leibnitz Zentrum in Germany.
All samples were examined at room temperature unless otherwise indicated.
XRD tests were performed using a PANalytical Xpert3 Powder X-ray diffractometer
over the range of 5° < 2θ < 60°. Each sample
was analyzed for 5 min at room temperature. The obtained Bragg peak
at 2θ ≈ 19° was fit to a Pearson and Lorentz function
to estimate its full width at half-maximum, from which the lamellar
thickness (L_c) can be derived according
to Scherrer’s equation.

Zhao D., Gimenez-Pinto V., Jimenez A.M., Zhao L., Jestin J., Kumar S.K., Kuei B., Gomez E.D., Prasad A.S., Schadler L.S., Khani M.M, & Benicewicz B.C. (2017). Tunable Multiscale Nanoparticle Ordering by Polymer Crystallization. ACS Central Science, 3(7), 751-758.

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SAXS Characterization of Copolymer Solutions

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SAXS measurements were conducted on a Bruker Nanostar U in the high resolution configuration (Brookhaven National Lab, Brookhaven, NY). The wavelength of the beam was 0.15418 with Cu rotating anode source. The nominal distance from sample to detector (Vantec 2000 area detector) was 1.1 m and the actual distance was calibrated with silver behenate before the measurements. Copolymer solutions were prepared at concentrations of 1% w/v in THF. Sample was loaded and sealed into glass capillaries (diameter 1.0 mm) to avoid solvent evaporation. The capillary was fixed in the sample holder and scattering data for each sample was collected for 6 h. SAXS data analysis was performed using the SasView 4.2.2 small-angle scattering analysis software (http://www.sasview.org/).

Yu X., Li G., Zheng B., Youn G., Jiang T., Quah S.P., Laughlin S.T., Sampson N.S, & Bhatia S.R. (2022). Controlling Rheology of Fluid Interfaces through Microblock Length of Sequence-Controlled Amphiphilic Copolymers. Macromolecular chemistry and physics, 223(18), 2200110.

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Small-Angle X-Ray Scattering Analysis

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Small angle X-ray scattering (SAXS) experiments were performed using a three-pinhole collimation Bruker NanostarU (Karlsruhe, Germany) system equipped with a copper anode IμS microsource. The scattering intensity was recorded using a Vantec-2000 with a 68 μm resolution detector. The scattering intensity I(q) is plotted as a function of the momentum transfer vector q = 4πsinθ/λ, where λ is the wavelength of the X-rays (Cu Kα radiation, 1.54 Å), and θ is half of the scattering angle. The sample-to-detector distance was 107 cm, allowing measurements with q values between 0.008 Å-1 and 0.2 Å-1. The angular scale was calibrated by the scattering peaks of the silver behenate standard.
The samples for SAXS analysis were prepared by the detachment of a small part of the polymeric mat from the metal support and it was mounted on a dedicated holder using Kapton tape. The samples were measured under constant temperature, 25 °C for 20,000 s. The raw data were corrected for the transmission coefficient and the incoherent scattering due to the Kapton tape and the background was subtracted in the data analysis using the SAXS Bruker A integrated software V4.1.45. The data analysis was carried out using ATSAS 2.5.1 [31 (link)].

Opalkova Siskova A., Sacarescu L., Opalek A., Mosnacek J, & Peptu C. (2023). Electrospinning of Cyclodextrin–Oligolactide Derivatives. Biomolecules, 13(2), 203.

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