Ab211292

Manufactured by Abcam

Sourced in China, United Kingdom

Ab211292 is an antibody product offered by Abcam. It is a primary antibody used for research purposes. The core function of this product is to detect and bind to a specific target in a sample.

Automatically generated - may contain errors

Lab products found in correlation

Dapi fluoromount g, by Southern Biotech (1 mentions) Ab15580, by Abcam (1 mentions) Ab6640, by Abcam (1 mentions) Ab22552, by Abcam (1 mentions) Sc 65495, by Santa Cruz Biotechnology (1 mentions) A32790, by Thermo Fisher Scientific (1 mentions) E cadherin, by Thermo Fisher Scientific (1 mentions) Sc 17781, by Santa Cruz Biotechnology (1 mentions) T4049, by Merck Group (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Ecl detection reagent, by Merck Group (1 mentions) Sab4500797, by Merck Group (1 mentions) Ab37168, by Abcam (1 mentions) Af7649, by R&D Systems (1 mentions)

5 protocols using ab211292

Longitudinal Tibia Analysis of Bone Development

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were sacrificed on PSD 3, 7, and 10. Thick (90 μm) longitudinal tibia frozen sections were prepared as described previously (Kusumbe et al., 2015 (link)). In brief, each tibia was stripped of soft tissues and fixed in 4% paraformaldehyde (PFA) at 4°C for 4 h, decalcified in 0.5 M EDTA at 4°C for 48 h, cryoprotected at 4°C for 48 h, and cryoembedded. Cryosections were prepared using a cryostat (Leica 3050S) with a thickness of 80 μm and stored at −80°C.
For immunostaining, the tibia sections were hydrated, permeabilized, blocked, and incubated with primary antibodies against endomucin (sc-65495, 1:100, Santa Cruz), osterix (ab22552, 1:200, Abcam), paired related homeobox 1 (PRRX1) (ab211292, 1:200, Abcam), KI67 (ab15580, 1:200, Abcam), and F4/80 (ab6640, 1:200, Abcam) overnight. Next day, the sections were incubated with anti-rabbit Alexa Fluor 488 (A32790, 1:300, Invitrogen) secondary antibody for 1 h at room temperature. Sections were washed with PBS and mounted with DAPI FluoroMount-G (0100-20, SouthernBiotech) and then sealed with coverslips.

Yang C., Li Z., Liu Y., Hou R., Lin M., Fu L., Wu D., Liu Q., Li K, & Liu C. (2022). Single-cell spatiotemporal analysis reveals cell fates and functions of transplanted mesenchymal stromal cells during bone repair. Stem Cell Reports, 17(10), 2318-2333.

+ Open protocol

+ Expand

Investigating Phosphorylation Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

[4-(5-amino-4-carbamoylimidazol-1-yl)-2,3-dihydroxycyclopentyl] methyl dihydrogen phosphate (ICA-1 T) (Therachem, Jaipur, India) and 8-hydroxy-1,3,6-naphthalenetrisulfonic acid (ζ-Stat) (NSC 37044) was provided by National Institute of Health (NIH, Bethesda, MD, USA). Sterile distilled water was used as the solvent. Materials were acquired as follows; primary antibodies of PKC-ζ (sc-17781, Santa Cruz Biotech), PKC-ι (610175, BD Biosciences), p-PKC-ι (T555, 44–968 G), p-PKC-ζ (T410, PA5-17837) and E-Cadherin (701134, Thermo Fisher Scientific), Vimentin (5741S), p-Vimentin (S39, 13614S), p-Vimentin (S56, 7391S), p-Smad2 (S465/467)/Smad3 (S423/425) (8828S) and SNAIL1 (3879S, Cell Signaling Biotechnology). PRRX1 (ab211292) and p-Vimentin (S71, ab115189, Abcam). p-Vimentin (S6, ADI-KAM-CC245-E) and p-Vimentin (S33, ADI-KAM-CC246-E, Enzo Life Sciences). β-actin-peroxidase (A3854, Sigma). Enhanced chemiluminescence solution (34,080, Pierce Inc.). siRNA (human small interfering RNA) for PKC-ζ (SR321432), PKC-ι (SR321426), SNAIL1 (SR304489, OriGene Inc.) and PRRX1 (AM16708, Thermo Fisher Scientific). DPBS without Mg²⁺ and Ca²⁺ ions (Dulbecco’s phosphate-buffered saline, D8537) and Trypsin–EDTA (Ethylenediaminetetraacetic acid, T4049, Sigma Aldrich).

Ratnayake W.S., Apostolatos C.A., Breedy S., Dennison C.L., Hill R, & Acevedo-Duncan M. (2021). Atypical PKCs activate Vimentin to facilitate prostate cancer cell motility and invasion. Cell Adhesion & Migration, 15(1), 37-57.

+ Open protocol

+ Expand

Immunohistochemical Evaluation of PRRX1 in Colorectal Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

IHC was performed on paraffin sections of normal colorectal and CRC tissues according to standard labelled streptavidin-biotin (LSAB) protocol (Dako) using primary antibodies against PRRX1 (Cat# ab211292, Abcam, China). The degree of staining in the sections was observed and scored independently by two pathologists. The percent positivity of PRRX1 staining was scored from 0 to 4: 0 (0%), 1 (1–25%), 2 (26–50%), 3 (51–75%), and 4 (>75%). The staining intensity was scored on a 4-point scale: 0 (no staining), 1 (weak staining, light yellow), 2 (moderate staining, yellowish-brown), and 3 (strong staining, brown). Subsequently, the PRRX1 expression score was calculated as the product of the percent positivity score and staining intensity score and ranged from 0 to 12. The final expression level of PRRX1 was defined as low [0–5] or high [6–12].

Zhong L., Tan W., Yang Q., Zou Z., Zhou R., Huang Y., Qiu Z., Zheng K, & Huang Z. (2022). PRRX1 promotes colorectal cancer stemness and chemoresistance via the JAK2/STAT3 axis by targeting IL-6. Journal of Gastrointestinal Oncology, 13(6), 2989-3008.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein samples were quantified by BCA method. Protein (30 µg) was mixed with loading buffer and denatured, followed by electrophoresis and transmembrane to polyvinylidene difluoride membrane (Merck, Darmstadt, Germany). Membranes were blocked with 5% skim milk at room temperature for 2 hours. After that, primary antibodies including anti-Prx1 (ab211292, 1:1,000, Abcam, Cambridge, UK), anti-FOXO3 (SAB2107951, 1:1,000, Sigma-Aldrich, St. Louis, MO, USA), anti-PI3K (GW21071, 1:500, Sigma-Aldrich), anti-p-PI3K (#SAB1305578, 1:1,000, Sigma-Aldrich), anti-Akt (SAB4500797, 1:1,000, Sigma-Aldrich), anti-p-Akt (#9271, 1:1,000, Cell Signaling Technology Danvers, MA, USA), and anti-GAPDH (ab37168, 1:1,000, Abcam) were used to incubate with the corresponding overnight at 4°C. After washing, membranes were incubated with goat antirabbit LgG (H + L) secondary antibody (1:1,000, Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd, Beijing, China). After washing, ECL detection reagent (Sigma-Aldrich, USA) was added to detect the signal. Relative expression level of each protein was normalized to endogenous control GAPDH using Image J software (https://imagej.nih.gov/ij/).

Sun X., Kong L., Li B., Zhang Y, & Yang H. (2018). Peroxiredoxin 1 silencing inhibited the growth and promoted apoptosis of pancreatic cancer cells via targeting FOXO3 gene. Cancer Management and Research, 10, 5019-5026.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Tibial Osteoprogenitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly dissected tibiae were fixed in 4% paraformaldehyde at 4°C for 4 hours and decalcified in 0.5 M EDTA, pH 7.5 at 4°C for 36 hours (Fig. 1b). Samples were cryoembedded, thick-sectioned, and stained with primary antibodies against osteoprogenitor marker Osx (1:100, sc-22536-R, Santa Cruz Bio, Dallas, Texas), proliferation marker Ki-67 (1:100, M-19, Santa Cruz, AF7649, R&D Systems), stem cells antigen-1 (Sca-1) (1:100, 14-5981-82, eBioscience, San Diego, California), Paired Related Homeobox 1 (Prrx1) (1:100, ab211292, Abcam, Cambridge, Massachusetts), and endothelial marker EMCN (1:100, V.7C7, Santa Cruz Bio). After three washes with PBS, the samples were stained with Alexa Fluor secondary antibodies from Donkey (1:400, ThermoFisher, Waltham, Massachusetts). Nuclei were stained with DAPI (0.5 μg/ml). The slides were mounted with Fluoromount-G and coverslipped before imaging.

Liu C., Cabahug-Zuckerman P., Stubbs C., Pendola M., Cai C., Mann K.A, & Castillo A.B. (2019). Mechanical loading promotes the expansion of primitive osteoprogenitors and organizes matrix and vascular morphology in long bone defects. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 34(5), 896-910.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!