CCK-8 kits (K1018, APExBIO, United States) were used to assess the viability of HT-29 cells. Cells at approximately 90% confluence in 96-well plates were treated with various doses of luteolin for 24 h. A total of 10 μL of CCK-8 reagent was added to each well and incubated for 1 h at 37°C in the dark. A PerkinElmer microplate reader was used to measure the absorbance at 450 nm (PerkinElmer VIC-TOR 1420, United States).
Cck 8 kit
Manufactured by Apexbio
Sourced in United States, China
The CCK-8 kit is a colorimetric assay used to determine the number of viable cells in cell proliferation and cytotoxicity assays. It utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce a yellow-colored formazan dye that can be quantified spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (4 mentions)
Inverted microscope, by Zeiss (2 mentions)
Multiskan fc microplate reader, by Thermo Fisher Scientific (2 mentions)
Multimode plate reader, by Thermo Fisher Scientific (2 mentions)
Victor 1420, by PerkinElmer (1 mentions)
Matrigel reagent, by BD (1 mentions)
Transwell insert, by Corning (1 mentions)
Microplate reader, by Tecan (1 mentions)
Infinite 200 pro, by Tecan (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Prism 8, by GraphPad (1 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
Phosphate buffered saline (pbs), by Solarbio (1 mentions)
Synergy htx multi mode microplate reader, by Agilent Technologies (1 mentions)
Apcin 1 444, by R&D Systems (1 mentions)
M200 pro, by Tecan (1 mentions)
Multimode plate reader, by Agilent Technologies (1 mentions)
Hct116 cells, by Cell Resource Center (1 mentions)
Sds page gel preparation kit, by Beyotime (1 mentions)
6 well plate, by Corning (1 mentions)
51 protocols using cck 8 kit
1
Assessing HT-29 Cell Viability
2
Cell Viability, Migration, Invasion, and Apoptosis Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCK-8 kits (K1018, Apexbio) were used to examine cell viability [23 (link)]. The plated cells (5 × 103 cells/well) were cultured for 1, 2, and 3 days and incubated with 10 µL of CCK-8 solution at 37 °C for 2 h, followed by OD value (at 450 nm) measurement.
Transwell inserts (pore size of 8 μm; Corning Incorporated, Corning, NY) uncoated (migration) or coated (invasion) with Matrigel reagent (BD Bioscience, San Diego, CA) were used for cell migration and invasion measurement [24 (link)]. After being stained with 0.1% crystal violet, cells were photographed under an inverted microscope (Carl Zeiss, Jena, Germany) and counted using ImageJ software.
Apoptosis was evaluated on the basis of the instructions of FITC-Annexin V cell apoptosis detection kit (4830-01-K, R&D Systems) [25 (link)], where Annexin-V-FITC and PI were mixed at a ratio of 1:2 to prepare Annexin-V-FITC/PI staining solution.
Transwell inserts (pore size of 8 μm; Corning Incorporated, Corning, NY) uncoated (migration) or coated (invasion) with Matrigel reagent (BD Bioscience, San Diego, CA) were used for cell migration and invasion measurement [24 (link)]. After being stained with 0.1% crystal violet, cells were photographed under an inverted microscope (Carl Zeiss, Jena, Germany) and counted using ImageJ software.
Apoptosis was evaluated on the basis of the instructions of FITC-Annexin V cell apoptosis detection kit (4830-01-K, R&D Systems) [25 (link)], where Annexin-V-FITC and PI were mixed at a ratio of 1:2 to prepare Annexin-V-FITC/PI staining solution.
3
Cell Viability Assessment via CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell viability was examined using CCK-8 kits (Apexbio, Houston, U.S.A.). Briefly, cells were plated into 96-well plates at a density of 5000 cells/well for 24 h and then treated with chemicals for different times. The CCK-8 solution was added to each well at a ratio of 1:5 to medium and cultured for 1 h. Then the OD value was evaluated at 450 nm. The relative OD value = OD value of experimental well/OD value of control well.
4
Cell Viability Assay with CCK-8 Kit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded into 96-well plates (1 × 103 cells/well) for 1-5 days. Specifically, the cells were treated for 12, 24, 48, and 96 h, whereupon 10 μL of CCK-8 solution was supplemented into each well of the plate for 1 h of incubation. The optical density (OD) values were tested at 450 nm at the designated time intervals using a microplate reader. Cell viability was assessed with the help of CCK-8 kits (K1018, Apexbio) with the formula of cell viability = (OD valuessample group − OD valuesblank group)/(OD valuesuntreated group − OD valuesblank group) [25 (link), 26 (link)]. The blank group was indicative of the OD value of the CCK-8 working buffer without cells.
5
Cell Proliferation Measurement with CCK8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CCK8 Kit (APExBIO Technology LLC) was adopted to measure cell proliferation as described by the manufacturer. In brief, 5 × 103 cells were cultured for 24 h in 96‐well plates, transfected, then inoculated in normal media. 10 μl of CCK8 were then introduced into every well and the cells incubated for 3 h. Cell proliferation rate was determined by measuring absorbance at 450 nm at 0, 24, 48, and 72 h on a microplate reader (Thermo Fisher).
6
CCK-8 Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability assay was done according to the instructions of CCK-8 kit (APExBIO). In
brief, every well that contained 200 μL medium was added with CCK-8 reagent (20 μL) into
the 96-well plate, followed by 4 h of incubation under 37°C. At last, we detected OD
(450 nm) values for diverse groups (n = 3). Cell viability was considered
to be 100% in control group (with no treatment) and that in other groups was determined on
this basis.
brief, every well that contained 200 μL medium was added with CCK-8 reagent (20 μL) into
the 96-well plate, followed by 4 h of incubation under 37°C. At last, we detected OD
(450 nm) values for diverse groups (n = 3). Cell viability was considered
to be 100% in control group (with no treatment) and that in other groups was determined on
this basis.
7
CCK-8 Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CCK-8 kit (Cat#: K1018) purchased from APExBIO (China) was used to investigate the cell viability. PC9 and Calu-3 cells at a density of 5,000 cells/well were added to 96-well plates. The CCK-8 solution was diluted to a ratio of 1:10 using the medium. At 0, 12, 24, 48, and 72 h, the medium was changed with 100 µL CCK-8 and incubated for 3 h at 37°C. Finally, the optical density (OD) was measured at 450 nm using a microplate reader (Tecan, Switzerland).
8
Radiation Dose-Response Assay on Lung Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Post-transduction, H460 and H1229 cells were seeded into 96-well plates at a density of 5 × 10^4 cells/well. They were subsequently exposed to varying radiation doses (0, 2, 4, and 6 Gy) for 48 h. Cell viability was determined using the CCK-8 kit (K1018, Apexbio). To each well, 10 μL of CCK-8 solution was added, and the cells were incubated for 2 h at 37 °C. The optical density (OD) value at 450 nm was measured using a Multiskan FC microplate reader (51,119,080, Thermo Fisher Scientific) to ascertain cell viability.
9
Cell Proliferation Quantification using CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was detected using the Cell Counting Kit-8 (CCK-8) assay. In brief, cells were seeded at the appropriate density in a 96-well plate and treated as described. Then, the CCK-8 assay was performed using a CCK-8 kit following the manufacturer’s protocol (Apexbio, USA), and then cell proliferation was quantified by detecting the optical density at 450 nm (OD450) on a microplate reader (Tecan, Infinite 200 PRO).
10
Irradiation-Induced Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transduced A549 and PC9 cells were seeded (1 × 104 cells/well) into 96-well plates, and then irradiated at different doses (0, 2, 4, 6 Gy) for 48 h, followed by the detection of cell viability using a CCK-8 kit (K1018, Apexbio, Shanghai, China). Briefly, 10 μL of CCK-8 solution was added to each well for 2-h incubation at 37 °C. A Multiskan FC microplate reader (51119080, Thermo Fisher Scientific) was adopted to examine optical density (OD) at a 450 nm wavelength.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!