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Xmd17 109

Manufactured by Carl Roth
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The XMD17-109 is a laboratory device designed for performing various analytical and experimental tasks. It is a multi-purpose instrument that can be utilized in a wide range of scientific applications. The core function of the XMD17-109 is to facilitate controlled and precise measurements, data collection, and experimental procedures in a laboratory setting.

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Lab products found in correlation

Eclipse ti, by Nikon (2 mentions) Escherichia coli o127 b8, by Merck Group (2 mentions) Plan fluor 20x objective, by Nikon (2 mentions)

2 protocols using xmd17 109

1

Aiptasia Larvae Infection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aiptasia larvae were washed and diluted to a concentration of 300-500 larvae ml-1 and were then incubated with 20 μg μl-1 LPS (lipopolysaccharides from Escherichia coli O127:B8, Sigma-Aldrich), 1 μM XMD17-109 (0.1% DMSO {Dimethylsulfoxid, Carl-Roth}), 0.1 % DMSO, or without for 1 hour. Microalgae cultures were then added to a final concentration of 1 x 105 microalgae ml-1 of the respective microalgal types and incubated at 26 °C and exposed to a 12L:12D cycle. After a 24-hour infection, the larvae were fixed in 4% formaldehyde for 30 minutes, washed in PBS, and mounted in 100% glycerol for counting. Infection status was quantified in at least 100 larvae per infection using a Nikon Eclipse Ti epifluorescent microscope using a Nikon Plan Fluor 20x objective, utilizing microalgal autofluorescence. Data recording was performed in Microsoft Excel v16.16.6.
Jacobovitz M.R., Rupp S., Voss P.A., Maegele I., Gornik S.G, & Guse A. (2021). Dinoflagellate symbionts escape vomocytosis by host cell immune suppression. Nature microbiology, 6(6), 769-782.
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2

Aiptasia Larvae Infection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aiptasia larvae were washed and diluted to a concentration of 300-500 larvae ml-1 and were then incubated with 20 μg μl-1 LPS (lipopolysaccharides from Escherichia coli O127:B8, Sigma-Aldrich), 1 μM XMD17-109 (0.1% DMSO {Dimethylsulfoxid, Carl-Roth}), 0.1 % DMSO, or without for 1 hour. Microalgae cultures were then added to a final concentration of 1 x 105 microalgae ml-1 of the respective microalgal types and incubated at 26 °C and exposed to a 12L:12D cycle. After a 24-hour infection, the larvae were fixed in 4% formaldehyde for 30 minutes, washed in PBS, and mounted in 100% glycerol for counting. Infection status was quantified in at least 100 larvae per infection using a Nikon Eclipse Ti epifluorescent microscope using a Nikon Plan Fluor 20x objective, utilizing microalgal autofluorescence. Data recording was performed in Microsoft Excel v16.16.6.
Jacobovitz M.R., Rupp S., Voss P.A., Maegele I., Gornik S.G, & Guse A. (2021). Dinoflagellate symbionts escape vomocytosis by host cell immune suppression. Nature microbiology, 6(6), 769-782.
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